For illustrating how extracellular polymeric substance (EPS) affected sludge water distribution, the present work comprehensively analyzed change of viscoelasticity and sludge network structure before/after EPS extraction, together with the sludge dewaterability and water distribution after biological, chemical and physical method conditioning. The results suggested the proportion of capillary water and adsorption water carried in soluble EPS (S-EPS) was 59.17% and 40.83%, and that in tightly-bound EPS (TB-EPS) was 54.77% and 45.23%, respectively. By contrast, the capillary water in loosely-bound EPS (LB-EPS) accounted for as high as 99.99%. Relative to raw sludge, adsorption water proportion in TB-EPS and S-EPS was reduced after lysozyme (LZM) or freezing-thaw conditioning, which was ascribed to reduction of EPS viscosity and the weakness of water adsorption capacity. Additionally, the sludge yield stress (τ y ) value rst reduced and then increased with the extraction of EPS, and the consistency coe cient (k) also decreased from 4.23 Pa•s n to 0.006 Pa•s n and then slightly increased after LZM conditioning. This observation indicated the sludge system became sensitive to shearing, and its network structural strength as well as colloid elasticity rst weakened and then slightly strengthened. What's more, after LZM or freezing-thaw conditioning, the sludge particle size signi cantly increased after TB-EPS extraction, while the sludge particle more easily absorbed water molecules, leading to the increase of the capillary water and adsorption water in sludge ocs. These changes reasonably explained the reason that sludge ltration performance was deteriorated after TB-EPS extraction.