Characterization of Antibody 444 Using Chromatographically Purified Enantiomers of Juvenile Hormones I, II, and III: Implications for Radioimmunoassays

Cusson, Michel; Miller, David F.; Goodman, Walter G.

doi:10.1006/abio.1997.2144

Cited by 20 publications

(12 citation statements)

References 18 publications

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“…The antiserum was raised in rabbits to 10R JH III, and has been shown to recognize JH from tobacco hornworm (Goodman et al, 1995;Cusson et al, 1997) and honeybee (Guidulgi et al, 2005) with high sensitivity by radioimmunoassay. Western blotting was performed on hemolymph proteins that were transferred to nitrocellulose membranes (Bio-Rad) after one-dimensional separation by 8% SDS-PAGE.…”

Section: Page and Immunological Proceduresmentioning

confidence: 99%

Two hexamerin genes from the termite Reticulitermes flavipes: Sequence, expression, and proposed functions in caste regulation

Zhou

Tarver

Bennett

et al. 2006

Gene

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Section: Page and Immunological Proceduresmentioning

confidence: 99%

Two hexamerin genes from the termite Reticulitermes flavipes: Sequence, expression, and proposed functions in caste regulation

Zhou

Tarver

Bennett

et al. 2006

Gene

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“…The antiserum was raised in rabbits to 10R JH III and has been shown to recognize JH from tobacco hornworm (Goodman et al, 1995;Cusson et al, 1997) and honeybee (Guidulgi et al, 2005) with high sensitivity by radioimmunoassay. The antibody is also useful for identification of JH that is covalently bound to denatured proteins (Zhou et al, 2006b).…”

Section: Western Blottingmentioning

confidence: 99%

Regulation of polyphenic caste differentiation in the termiteReticulitermes flavipesby interaction of intrinsic and extrinsic factors

Scharf

Buckspan

Grzymala

et al. 2007

Journal of Experimental Biology

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SUMMARY Polyphenism is a key strategy used by solitary insects to adapt to changing environmental conditions and by eusocial insects for existing collaboratively in a social environment. In social insects, the morphogenetic juvenile hormone(JH) is often involved in directing the differentiation of polyphenic behavioral castes. The present study examines the effects of JH, environment and feeding on caste polyphenism in a eusocial insect, the termite Reticulitermes flavipes (Kollar). Our approach included a combination of model JH bioassays, SDS-PAGE and western blotting. Our findings revealed significant temperature-dependent effects on (1) JH-induced soldier caste differentiation, (2) abundance of soldier-inhibitory hexamerin proteins and(3) JH-sequestration by hexamerin proteins. Additionally, although it appears to be dependent on a complex interaction of factors, feeding apparently plays a significant upstream role in enhancing hexamerin accumulation under normal colony conditions. These findings offer important new information on termite eusocial polyphenism by providing the first mechanistic evidence linking an intrinsic caste regulatory factor (hexamerin proteins) to an upstream extrinsic factor (environment) and a downstream response (caste differentiation). These observations are consistent with the hypothesis that the hexamerins serve as an environmentally and nutritionally responsive switching mechanism that regulates termite caste polyphenism.

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“…Racemic [10-3 H]JH I, II, and III were enantiomerically purified separately (7). Baseline separation of (10R,11S) natural enantiomer (fast eluting) and (10S,11R) unnatural enantiomer (slower eluting) was achieved for racemic [10-3 H]JH I and II using a Haskel pump connected to a Chiralpak AS "guard" column (4.6 ϫ 50 mm) eluted at 0.5 ml/min.…”

Section: [10-3 H] Jh and Jh Diol Enantiomeric Purificationmentioning

confidence: 99%

“…Subsequently, this group (6) devised a shorter (7-step) synthesis of (10R)- [12-3 H]JH III starting from methyl farnesoate, again in a 97:3 mix of enantiomers, using the Sharpless asymmetric epoxidation. Cusson et al (7) presented an attractive method of totally resolving enantiomers of JH I-III by chiral chromatography, utilizing nonlabeled forms of the JH to study binding specificities of an antibody to JH.…”

mentioning

confidence: 99%

“…The threochlorohydrin gives the natural cis-epoxide ring upon treatment with alcoholic carbonate. Each labeled JH can be easily separated into optically pure enantiomers by chiral chromatography using a modified method of Cusson et al (7). In addition, separation of the chiral products using normal phase LC on a silica column eluted slowly with a weak solvent results in a good, but incomplete separation of the unlabeled JH from the [10-3 H]JH.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous Preparation of Both Enantiomers of Juvenile Hormones Labeled at C-10 with Tritium at High Specific Activity

Maxwell

Anderson

Schooley

2002

Analytical Biochemistry

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Characterization of Antibody 444 Using Chromatographically Purified Enantiomers of Juvenile Hormones I, II, and III: Implications for Radioimmunoassays

Cited by 20 publications

References 18 publications

Two hexamerin genes from the termite Reticulitermes flavipes: Sequence, expression, and proposed functions in caste regulation

Two hexamerin genes from the termite Reticulitermes flavipes: Sequence, expression, and proposed functions in caste regulation

Regulation of polyphenic caste differentiation in the termiteReticulitermes flavipesby interaction of intrinsic and extrinsic factors

Simultaneous Preparation of Both Enantiomers of Juvenile Hormones Labeled at C-10 with Tritium at High Specific Activity

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