Characterization of antibody covalently coupled to liposomes

Chemically Modified Human Immunoglobulin G: Hydrophobicity and Surface Activity at Air/Solution Interface

“…It is apparent from the kinetic curves that the maximum reduction in surface tension at both concentrations studied is higher for the modified than for the native proteins. Thus, at concentrations of 0.1 mg/ml after 23 h the observed surface tension for 9C 8 -, 25C 8 -, and 25C 16 -IgG, is about 48 mN/m, whereas for the native IgG it is about 51 mN/m. Moreover, it appears that the modified proteins adsorb faster than the native IgG: for native IgG at 0.1 mg/ml, 6 h are required to lower the surface tension to 53 mN/m, compared to 3 h and to 20 min in the case of C 8 -and C 16 -modified IgGs, respectively.…”

“…This type of modification can also lead to unique applications in the field of biosensors, drug targeting, immunoassays, and immobilized affinity ligand chromatography (2-7). For example, Huang et al (8,9) reported on the attachment of hydrophobic chains to the IgG molecules, which were then introduced into liposomes for the purpose of drug targeting. We have found that hydrophobized human IgG retains high specific recognition ability in ELISA tests; i.e., modification by alkyl chains did not influence noticeably the native structure of the protein (10).…”

A review of mechanistic insight and application of pH-sensitive liposomes in drug delivery

“…The pH-sensitive liposomes containing herpes simplex virus thymidine kinase gene released content into the cytoplasm and delivered DNA expressed efficiently in the cells (Wang & Huang, 1986). To improve binding efficiency, target cells can be improved by using immunoliposomes (Huang et al, 1982). Wang & Huang (1987) reported immunoliposomes that bind to major histocompatibility antigen H2-Kk for the delivery of model gene Escherichia coli chloramphenicol acetyltransferase (CAT).…”

Enhanced delivery to target cells by heat-sensitive immunoliposomes.

“…Isolation, iodination, and derivatization of mouse monoclonal anti-H2Kk IgG with the palmitic acid ester ofN-hydroxysuccinimide have previously been described (8,19,22 Preparation of Immunoliposomes with Entrapped Uridine. Immunoliposomes were prepared as previously described (19).…”