Characterization of antidiabetic effects of Dendrobium officinale derivatives in a mouse model of type 2 diabetes mellitus

Peng, Dong; Tian, Wenni; An, Miaoqing; Chen, Yanlan; Zeng, Wenshen; Zhu, Siyang; Li, Pan; Du, Bing

doi:10.1016/j.foodchem.2022.133974

Cited by 28 publications

(5 citation statements)

References 41 publications

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“…In this research, a total of 32 compounds were identifed by UPLC Triple-TOF MS/MS, comprising favonoids, steroids, alkaloids, amino acids, coumarins, and organic acids (Table 1). Obviously, polysaccharide is the predominant active components that accord with Hong-Yan Wang's research [20][21][22]. At the same time, the favonoid extract showed an important part in hypoglycemic and hypolipidemic efects, which had been demonstrated previously [23][24][25].…”

Section: Discussionsupporting

confidence: 71%

Regulation of Glucolipid Metabolism in Mice Using Dendrobium huoshanense Extract and PI3k/Akt Signaling Pathway Activation

Zhang,

Fang,

et al. 2023

Journal of Food Biochemistry

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The present research is carried out to study the hypoglycemic and hypolipidemic effects of the Dendrobium huoshanense C. Z. Tang et S. J. Cheng extract on type 2 diabetic mice. D. huoshanense extract was prepared using the condensation reflux method. The chemical components were separated by UPLC Triple-TOF MS/MS to further identify the components that mainly exerted hypoglycemic and hypolipidemic effects. Type 2 diabetic C57BL/6 mice were induced by a high-fat diet (HFD) followed by intraperitoneal streptozotocin (STZ) and were daily gavaged with different doses of D. huoshanense extract for 5 weeks. Various methods such as HE staining, ELISA, and Western blot were used to evaluate the hypoglycemic and hypolipidemic effects of D. huoshanense extract on mice with type 2 diabetes mellitus model. The experimental results revealed that the main compounds of D. huoshanense extract were polysaccharides, flavonoids, alkaloids, amino acids, and many other chemical components. Oral administration of 200 mg/kg of the extract of D. huoshanense significantly reduced blood glucose and insulin levels of high-dose group mice and lowered total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) levels, but increased high-density lipoprotein cholesterol (HDL-C) levels and liver glycogen content. In addition, the protein expression levels of insulin receptor (Ins R), phosphatidylinositol-3 kinase (PI3K) and p-protein kinase B (Akt) were upregulated. These results imply that the D. huoshanense aqueous extract can improve insulin resistance and lipid metabolism in type 2 diabetic mice, and it may regulate the function through the PI3K/AKT pathway.

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Section: Discussionsupporting

confidence: 71%

Regulation of Glucolipid Metabolism in Mice Using Dendrobium huoshanense Extract and PI3k/Akt Signaling Pathway Activation

Zhang,

Fang,

et al. 2023

Journal of Food Biochemistry

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“…Previous research has shown that Faecalibaculum has the capacity to deactivate glucose transporter type 1 (GLUT1), which has a positive impact on the treatment of T2DM. 44 Furthermore, Faecalibaculum is negatively correlated with TC, TG, and LDL-C. 45 In our study, the observed decrease in TC, TG, and LDL-C levels in the treatment groups could potentially be attributed to the increased abundance of Faecalibaculum . T2DM leads to an increase in the abundance of Corynebacterium , Proteus , and Enterococcus.…”

Section: Discussionmentioning

confidence: 51%

“…53 It is worth noting that Blautia, Akkermansia, and Faecalibaculum are commonly known as significant contributors to the production of SCFAs. 35,45,54 Akkermansia mainly produces acetate. 55 Bacteria within the genus Blautia mainly produce acetate and butyrate.…”

Section: Food and Function Papermentioning

confidence: 99%

The synergism of Lactobacillaceae, inulin, polyglucose, and aerobic exercise ameliorates hyperglycemia by modulating the gut microbiota community and the metabolic profiles in db/db mice

Li,

Tong,

Shao

et al. 2024

Food Funct.

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“…The DOP mainly consisted of mannose and glucose with the dominant proportions of 85.75 and 12.82% and contained small amounts of galacturonic acid, galactose, arabinose, and glucuronic acid with the molar ratios of 0.50:0.46:0.43:0.04, respectively (Figure 2B), which was inconsistent with previous reports and may be because of the differences in raw material sources, extraction condition, and preliminary purification methods of polysaccharide. 13,29 3.2. Establishment of T2DM Mice and Pseudo-Germ-Free Mice.…”

Section: Chemical Component Assay Of Dopmentioning

confidence: 99%

Dendrobium officinale Polysaccharide Alleviates Type 2 Diabetes Mellitus by Restoring Gut Microbiota and Repairing Intestinal Barrier via the LPS/TLR4/TRIF/NF-kB Axis

Chen

2023

J. Agric. Food Chem.

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Dendrobium officinale polysaccharide (DOP), the main active component, has a variety of bioactivities. In this study, a type 2 diabetes mellitus (T2DM) and antibiotic-induced pseudo-germ-free mouse models were used to investigate the hypoglycemic mechanisms of DOP. The findings showed that DOP ameliorated dysfunctional glucolipid metabolism, lipopolysaccharide (LPS) leakage, and metabolic inflammation levels in T2DM mice. Furthermore, DOP significantly upregulated the mRNA expression of tight junction proteins Claudin-1, Occludin, and ZO-1 and reduced intestinal inflammation and oxidative stress damage through the LPS/TLR4/TRIF/NF-κB axis to repair the intestinal barrier. Interestingly, pseudo-germ-free mouse experiments confirmed that the above beneficial effects of DOP were dependent on gut microbiota. 16S rRNA analysis showed that DOP strongly inhibited the harmful bacterium Helicobacter by 94.57% and facilitated the proliferation of probiotics Allobaculum, Bifidobacterium, and Lactobacillus by 34.96, 139.41, and 88.95%, respectively. Therefore, DOP is capable of rebuilding certain specific intestinal microbiota to restore intestinal barrier injury, which supports the utilization of DOP as a new type of prebiotic in functional foods for T2DM.

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Characterization of antidiabetic effects of Dendrobium officinale derivatives in a mouse model of type 2 diabetes mellitus

Cited by 28 publications

References 41 publications

Regulation of Glucolipid Metabolism in Mice Using Dendrobium huoshanense Extract and PI3k/Akt Signaling Pathway Activation

Regulation of Glucolipid Metabolism in Mice Using Dendrobium huoshanense Extract and PI3k/Akt Signaling Pathway Activation

The synergism of Lactobacillaceae, inulin, polyglucose, and aerobic exercise ameliorates hyperglycemia by modulating the gut microbiota community and the metabolic profiles in db/db mice

Dendrobium officinale Polysaccharide Alleviates Type 2 Diabetes Mellitus by Restoring Gut Microbiota and Repairing Intestinal Barrier via the LPS/TLR4/TRIF/NF-kB Axis

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