Characterization of antigen-presenting cells from the porcine respiratory system

López-Robles, Guadalupe; Silva‐Campa, Erika; Burgara-Estrella, Alexel; Hernández, Jesús

doi:10.1016/j.rvsc.2015.04.009

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…8). Even if its immune system is one of the best characterized after the human, primate, and murine ones, 9 so far the few studies that have described pig's lung immune cells did not enter in dendritic cell (DC) and macrophage (My) subpopulation details, 10,11 limiting its use as a preclinical model for respiratory pathologies.…”

Section: Introductionmentioning

confidence: 99%

The respiratory DC/macrophage network at steady-state and upon influenza infection in the swine biomedical model

Maisonnasse¹,

Bouguyon²,

Piton³

et al. 2016

Mucosal Immunology

129

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Human and mouse respiratory tracts show anatomical and physiological differences, which will benefit from alternative experimental models for studying many respiratory diseases. Pig has been recognized as a valuable biomedical model, in particular for lung transplantation or pathologies such as cystic fibrosis and influenza infection. However, there is a lack of knowledge about the porcine respiratory immune system. Here we segregated and studied six populations of pig lung dendritic cells (DCs)/macrophages (Mθs) as follows: conventional DCs (cDC) 1 and cDC2, inflammatory monocyte-derived DCs (moDCs), monocyte-derived Mθs, and interstitial and alveolar Mθs. The three DC subsets present migratory and naive T-cell stimulation capacities. As observed in human and mice, porcine cDC1 and cDC2 were able to induce T-helper (Th)1 and Th2 responses, respectively. Interestingly, porcine moDCs increased in the lung upon influenza infection, as observed in the mouse model. Pig cDC2 shared some characteristics observed in human but not in mice, such as the expression of FCɛRIα and Langerin, and an intra-epithelial localization. This work, by unraveling the extended similarities of the porcine and human lung DC/Mθ networks, highlights the relevance of pig, both as an exploratory model of DC/Mθ functions and as a model for human inflammatory lung pathologies.

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Section: Introductionmentioning

confidence: 99%

The respiratory DC/macrophage network at steady-state and upon influenza infection in the swine biomedical model

Maisonnasse¹,

Bouguyon²,

Piton³

et al. 2016

Mucosal Immunology

129

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“…In this study, the cDCs were described as CD172 + CD11R1 + CD1 +/− CD80/CD86 +/− , and the spleen was the tissue with the highest frequency of cDCs. Other study evaluated the phenotypic and functional properties of antigen presenting cells (MHC-II high CD172a + ) from the mediastinal lymph node (Lopez-Robles et al, 2015). Unfortunately, no additional studies on the classification of cDCs in lymph nodes, much less the characterization of the cDC1 and cDC2 in these tissues, have been reported.…”

Section: Introductionmentioning

confidence: 99%

Characterization and expression of DEC205 in the cDC1 and cDC2 subsets of porcine dendritic cells from spleen, tonsil, and submaxillary and mesenteric lymph nodes

Parra-Sánchez

Puebla-Clark

Reséndiz

et al. 2018

Molecular Immunology

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Conventional dendritic cells (cDCs) are divided into the following different subtypes: cDC1, which promotes a Th1 response, and cDC2, which stimulates a Th2 and Th17 response. These cells have not been characterized in porcine lymphoid tissues. DEC205 is a receptor that increases antigen presentation and allows DCs to cross-present antigens. The objectives of this work were to characterize cDCs subsets in the tonsil, submaxillary and mesenteric lymph nodes and spleen lymphoid tissues and to determine their expression of DEC205 by flow cytometry. The cDC1 (MHCIICADM1CD172a) and cDC2 (MHCIICADM1CD172a) phenotypes were confirmed by the expression of characteristic cDC1 and cDC2 transcripts (FLT3, XCR1 and FCER1α). Among all lymphoid tissues, the spleen had the highest frequency of total cDCs. The cDC1:cDC2 ratio showed that all lymph tissues had higher levels of cDC1 than levels of cDC2. DEC205 cDCs were found in all analyzed tissues, albeit with different frequencies. Our research will facilitate the study on the function of these cells and the investigation of the strategies for DEC205 targeting and functional studies.

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“…Peptide presentation to T helper (CD4 + ) and cytotoxic T lymphocytes (CTLs, CD8 + ) by major histocompatibility complexes (MHCs) is essential for adaptive immunity in vertebrates [ 1 – 3 ]. MHC class I and II molecules represent intracellular and extracellular peptides, respectively [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Analysis of peptide-SLA binding by establishing immortalized porcine alveolar macrophage cells with different SLA class II haplotypes

Cho

et al. 2018

Vet Res

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Primary porcine alveolar macrophages (PAM) are useful for studying viral infections and immune response in pigs; however, long-term use of these cells is limited by the cells’ short lifespan. We immortalized primary PAMs by transfecting them with both hTERT and SV40LT and established two immortalized cell lines (iPAMs) actively proliferating even after 35 passages. These cells possessed the characteristics of primary PAMs, including strong expression of swine leukocyte antigen (SLA) class II genes and the inability to grow anchorage-independently. We characterized their SLA genes and subsequently performed peptide-SLA binding assays using a peptide from porcine circovirus type 2 open reading frame 2 to experimentally measure the binding affinity of the peptide to SLA class II. The number of peptides bound to cells measured by fluorescence was very low for PK15 cells (7.0% ± 1.5), which are not antigen-presenting cells, unlike iPAM61 (33.7% ± 3.4; SLA-DQA*0201/0303, DQB1*0201/0901, DRB1*0201/1301) and iPAM303 (73.3% ± 5.4; SLA DQA*0106/0201, DQB1*0202/0701, DRB1*0402/0602). The difference in peptide binding between the two iPAMs was likely due to the allelic differences between the SLA class II molecules that were expressed. The development of an immortal PAM cell panel harboring diverse SLA haplotypes and the use of an established method in this study can become a valuable tool for evaluating the interaction between antigenic peptides and SLA molecules and is important for many applications in veterinary medicine including vaccine development.Electronic supplementary materialThe online version of this article (10.1186/s13567-018-0590-2) contains supplementary material, which is available to authorized users.

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Characterization of antigen-presenting cells from the porcine respiratory system

Cited by 4 publications

References 31 publications

The respiratory DC/macrophage network at steady-state and upon influenza infection in the swine biomedical model

The respiratory DC/macrophage network at steady-state and upon influenza infection in the swine biomedical model

Characterization and expression of DEC205 in the cDC1 and cDC2 subsets of porcine dendritic cells from spleen, tonsil, and submaxillary and mesenteric lymph nodes

Analysis of peptide-SLA binding by establishing immortalized porcine alveolar macrophage cells with different SLA class II haplotypes

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