Characterization of antimicrobial resistance of recentSalmonella entericaserovar Gallinarum isolates from chickens in South Korea

Abstract

Salmonella enterica serovar Gallinarum isolates (n 0105) from chickens in South Korea between 2002 and 2007 were tested for antimicrobial susceptibility by determining minimum inhibitory concentrations of 16 antimicrobials, and their predominant resistance profiles were genetically characterized. Most isolates (99/ 105; 94.3%) were resistant to nalidixic acid and resistant/intermediately resistant to fluoroquinolones, and 63.8% (67/105) of the isolates were resistant to three or more antimicrobials. Forty-two quinolone-resistant isolates, of which the quinolone resistance-determining regions of the gyrA genes were sequenced, contained a substitution of a Ser to a Phe or Tyr at position 83 (71.4%), or a substitution of an Asp to an Asn, Gly, or Tyr at position 87 (28.6%). Fifty-seven sulphamethoxazole-resistant isolates were tested for the presence of class 1 integrons by polymerase chain reaction, and their resistance gene cassettes were analysed by sequencing. Three different class 1 integrons containing the resistance-gene insert aadA (52.6%; n 030), aadB (12.3%; n07), or aadB-aadA (12.3%; n 07) were identified. Most isolates harbouring the integron containing aadB-aadA displayed resistance to all three aminoglycosides tested and also showed increased resistance to fluoroquinolones. These findings suggest that fluoroquinolone resistance may be epidemiologically linked to multiple aminoglycoside resistance.

Antimicrobial susceptibility of Salmonella Gallinarum and Salmonella Pullorum isolated from ill poultry in Brazil

“…In South Korea, no resistance to fluoroquinolones was detected in S. Gallinarum isolates from chickens from 1995 (LEE et al 2004), however a number of isolates from 2001 were reported resistant to fluoroquinolones showing an increased MIC that ranged from 2 to 8 µg/ml (KANG et al 2010). Our results also suggest an increasing resistance to this class of antibiotics, especially in S. Gallinarum which was statistically different from the levels detected in older isolates (P<0.05).…”

Risk factors for antimicrobial resistance in Escherichia coli isolates from poultry in Haryana

“…DNA was extracted using method as previously described (Kang et al, 2010) with some modifications. Various E. coli isolates was assigned to different phylogenetic groups according to method described by Clermont et al (2000) with some modifications using polymerase chain reaction (PCR).…”

Further investigations on the epidemiology of fowl typhoid in Brazil

“…In Brazil, the use of fluoroquinolones to treat flocks with FT is also a common practice in egg-producing farms (Penha Filho et al, 2016). Multi-resistance has been found in strains from Korea (Lee et al, 2003;Kang et al, 2010).…”