Characterization of Antineutrophil Antibodies in Patients with Neutropenia Associated with Nutritional Copper Deficiency

Abstract

Six antineutrophil antibody (ANA)-positive patients with copper deficiency were classified into two groups; those with (group A, n =3) and those without (group B, n = 3) neutropenia. The percent binding of ANA for normal peripheral neutrophils was similar in both groups (8 3.5 ± 7.2 vs. 79.1 ± 10.5%). The percent binding of sera to cultured promyelocytic leukemia cells (HL-60) was increased in group A (from 3.7 ± 3.2 to 12.2 ± 2.3%) but not in group B (from 65.3 ± 21.7 to 40.7 ± 6.3%) after stimulation of HL-60 with DMSO. The stimulated HL-60 cells expressed CD 16 and CD 11b antigens. In the presence of monoclonal antibody for CD 16, the titer of ANA was nil in group A and unchanged in group B. Thus, ANA of patients with neutropenia may recognize mainly the CD 16 antigen, the Fcγ receptor III of neutrophils.

Clinical Features of Hematological Disorders Caused by Copper Deficiency during Long-Term Enteral Nutrition

“…Moreover, there are reports that in a copper-deficient state, the maturation of granulocytic stem cells is suppressed in vitro (23) and heme production by heme synthetase is disturbed (24). On the other hand, there is a report suggesting that anti-neutrophil antibody is responsible for copper deficiency neutropenia (25). In this report, the neutrophil count was normalized and anti-neutrophil antibody production became negative or decreased after copper supplementation therapy.…”

Clinicopathological Analysis of Hematological Disorders in Tube-Fed Patients with Copper Deficiency

“…In our search of the Pub Med database, we found no reports of electron microscopic examination of copper deficiency in humans. Dallman and Goodman (16) (27) and antineutrophil antibody (28). It has been proposed that the anemia induced by copper deficiency is caused by a decrease in the activity of copper-dependent enzymes.…”

Neutropenia in congenital nephrotic syndrome of the Finnish type: role of urinary ceruloplasmin loss

“…2 It is noteworthy that copper deficiency related neutropenia can be related to anti-neutrophil antibodies. 3 Copper deficiency may worsen anemia in patients with kidney failure 4 ; however, it has never been observed in relation to nephrotic proteinuria responsible for urinary ceruloplasmin loss. The patient was born at 37 weeks gestational age.…”