Characterization of Antioxidant Activity ofMomordica CharantiaFruit by Infrared-Based Fingerprinting

Abstract

Momordica charantia is widely consumed edible fruit. The food and pharmaceutical industries use it as a natural antioxidant. However, the quality control of M. charantia-based medicinal products is questionable due to the complexity of metabolites in this fruit. Hence, this study has developed a statistical model in predicting the antioxidant value through the 2, 2-diphenyl-1 picrylhydrazyl radical scavenging activity and ferric reducing antioxidant power based on infrared spectroscopy with attenuated total reflectance. This technique was reliably used for quality control. Six ethanol extracts (0, 20, 40, 60, 80, and 100% in water) of this plant's fruit were prepared. The radical scavenging and ferric reducing antioxidant power activities were measured and the chemical profiling of the extracts was fingerprinted by infrared spectroscopy between 4,000 and 600 cm−1 at a resolution of 4 cm−1. Statistical analysis was developed by correlating the bioactivity and infrared spectra of each extract using orthogonal partial least square discriminant analysis. The C-N, C˭O, CO , C-H, and OH infrared signals were positively correlated with biological activity. The antioxidant activity of the fruit of M. charantia may be due to the presence of several antioxidants that work synergistically.

Correlation of FT-IR Fingerprint and α-Glucosidase Inhibitory Activity of Salak (Salacca zalacca) Fruit Extracts Utilizing Orthogonal Partial Least Square

“…The percentage yield followed this trend 80% > 60% > 0% > 40% > 20% > 100%. The result was in agreement with [ 26 , 27 ].…”

Alpha-Glucosidase Inhibitory Effect of Psychotria malayana Jack Leaf: A Rapid Analysis Using Infrared Fingerprinting

“…This result further indicated that a high proportion of methanol could be the perfect solvent for increasing extraction yields. This is in agreement with Khatib et al [ 14 ], where a higher yield of extraction was related to the water–alcohol combinations rather than the monocomponent solvent. Sultana et al [ 15 ] also reported that aqueous methanol is the most suitable solvent for extracting the leaves of a medicinal plant with a higher yield of extraction.…”

Optimization of Bioactive Substances in the Wastes of Some Selective Mediterranean Crops

“…Figure 5 demonstrates the FTIR spectra of the selected waste extracts obtained at optimum conditions, which comprises the range between 4000 and 500 cm −1 . The peaks ranging from 3010 to 3670 cm −1 correspond to the O-H stretch of the hydroxyl group, comprising biophenols and alcohols [47]. The sharper peaks observed between 3409 and 1733 cm −1 are assigned to C=O and O-H stretchings, which are attributable to oleuropein, naringin and other phenolic compounds of the leaf and peel extracts [48].…”