Characterization of atypical Enteropathogenic Escherichia coli (aEPEC) isolated from dogs

Almeida, Paula Maria Pereira de; Arais, Lavicie Rodrigues; Andrade, João Ramos Costa; Prado, E.; Irino, Kinue; Cerqueira, Aloysio de Mello Figueiredo

doi:10.1016/j.vetmic.2012.02.021

Cited by 22 publications

(16 citation statements)

References 26 publications

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“…EPEC is characterized by the production of intimin (eae) and it can be classified as typical or atypical based on the presence or absence of bundle-forming pili (bfp), respectively (MAINIL, 2013). Our results are in accordance with other studies and suggested that EPEC are important diarrheagenic E. coli in dogs, especially atypical EPEC (NAKAZATO et al, 2004;ALMEIDA et al, 2012;PUÑO-SARMIENTO et al, 2013). In addition, EPEC were mainly classified in phylogroups B1 and E, as reported in previously published studies in zoo animals, dogs, pigs, and calves (BALDY-CHUDZIK et al, 2008;TRAMUTA et al, 2008;.…”

supporting

confidence: 93%

“…This method is only capable of determining phylogroups A, B1, B2, and D (HARADA et al, 2012;SALVARANI et al, 2012;SCHMIDT et al, 2015) instead of the seven phylogroups that were detected in this study. In Brazil, only two studies identified E. coli pathovars associated with diarrhea in dogs (ALMEIDA et al, 2012;PUÑO-SARMIENTO et al, 2013), but none determined the phylogenetic groups of E. coli. Therefore, this study aimed to detect the virulence genes, pathovars, and phylogenetic groups of E. coli strains isolated from the feces of dogs with and without diarrhea.…”

Section: Introductionmentioning

confidence: 99%

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Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil

et al. 2018

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Detecção de genes de virulência e grupos filogenéticos de amostrasEscherichia coli isoladas de cães no Brasil INTRODUCTIONEscherichia coli are a component of normal intestinal microbiota in humans and other animals. Phenotypic and genotypic characteristics allow the identification of E. coli pathogenic strains or pathovars (COURA et al., 2014). Dogs are colonized by E. coli during the first days of their life and some strains can cause enteric or extra-intestinal infections (BEUTIN, 1999 (BEUTIN, 1999). Other pathovars are associated with diarrhea in other animals, namely enteroaggregative E. coli (EAEC) and enteroinvasive E. coli (EIEC) (MAINIL, 2013). Because of the close contact with humans, dogs could increase the risk of transmitting potentially zoonotic microorganisms, such as EPEC and STEC (BEUTIN, 1999).In addition to the pathotype and pathovar classification, E. coli strains can be assigned to one of the seven phylogenetic groups, including A, B1, B2, C, D, E, and F (CLERMONT et al., 2013). E. coli genomic structure has shown that the strains belonging to different phylogroups are associated with the disease state and source of isolation (CLERMONT et al., 2013). The extraintestinal pathogenic E. coli (ExPEC) strains are clustered mostly in groups B2 and D and intestinal pathogenic E. coli are mostly in phylogroups A, B1, and E (ESCOBAR- PÁRAMO et al., 2004; CLERMONT et al., 2011). Although phylogenetic characterization is an important tool to improve the understanding of E. coli populations and the relationship between strains and disease (CLERMONT et al., 2011;COURA et al., 2015), few studies have determined phylogenetic groups of E. coli isolated from dogs worldwide, and of those that did, the most frequently used method was a triplex PCR method developed by CLERMONT in 2000. This method is only capable of determining phylogroups A, B1, B2, and D (HARADA et al., 2012;SALVARANI et al., 2012;SCHMIDT et al., 2015) instead of the seven phylogroups that were detected in this study. In Brazil, only two studies identified E. coli pathovars associated with diarrhea in dogs (ALMEIDA et al., 2012;PUÑO-SARMIENTO et al., 2013), but none determined the phylogenetic groups of E. coli. Therefore, this study aimed to detect the virulence genes, pathovars, and phylogenetic groups of E. coli strains isolated from the feces of dogs with and without diarrhea. MATERIALS AND METHODS Stool samplesStool samples were collected from 154 dogs, of which 92 were diarrheic and 62 were without diarrhea. Samples from diarrheic dogs were obtained directly from the rectum, at the Veterinary Hospital of Universidade Federal de Minas Gerais (Belo Horizonte city), upon admission. Collections were performed from dogs that were undergoing a consultation for the occurrence of diarrhea. With owner's consent, fecal samples from non-diarrheic animals were obtained from the dogs of students attending the Universidade Federal de Minas Gerais. For non-diarrheic dogs, fecal samples were either taken directly from the rectum or after spontaneous ...

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supporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil

et al. 2018

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“…The fact that virulence genes were detected in the isolates investigated indicates that they were pathogenic E. coli strains (Bentancor et al 2007, Shahrani et al 2014. Although the serotypes of the isolates in this study were not determined, they could belong to the serogroups capable of causing zoonotic infections (Morato et al 2009, de Almeida et al 2012, Tramuta et al 2014. The 47.1% pathogenic E. coli prevalence in this study is higher when compared with 44.4, 25 and 37.1% faecal pathogenic E. coli prevalence among 45, 68 and 70 dogs with diarrhoea reported in Canada (Hammemermulaer et al 1995), Brazil (Puno-Sarmiento et al 2013) and Egypt (Ali and Metwaly 2015), respectively.…”

Section: Discussionmentioning

confidence: 69%

“…Animals with clinical conditions such as diarrhoea usually have immune suppression which favours increased faecal shedding of E. coli (de Almeida et al 2012). Diarrhoeic animals defecate frequently and uncontrollably, thus they tend to spread E. coli more than the non-diarrhoeic ones.…”

Section: Introductionmentioning

confidence: 99%

Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran

Torkan¹,

Bahadoranian²,

Khamesipour³

et al. 2016

Arch. med. vet.

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“…E. coli has been associated with systemic sepsis (9). E. coli strains from various animal species have been investigated extensively (10)(11)(12)(13)(14). However, little is known about the distribution and characteristics of E. coli in giant pandas.…”

mentioning

confidence: 99%

Serotypes, Virulence Factors, and Antimicrobial Susceptibilities of Vaginal and Fecal Isolates of Escherichia coli from Giant Pandas

Wang

Yan

Xia

et al. 2013

Appl Environ Microbiol

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f Although Escherichia coli typically colonizes the intestinal tract and vagina of giant pandas, it has caused enteric and systemic disease in giant pandas and greatly impacts the health and survival of this endangered species. In order to understand the distribution and characteristics of E. coli from giant pandas, 67 fecal and 30 vaginal E. coli isolates from 21 giant pandas were characterized for O serogroups, phylogenetic groups, antimicrobial susceptibilities, and pulsed-field gel electrophoresis (PFGE) profiles. In addition, these isolates were tested for the presence of extraintestinal pathogenic E. coli (ExPEC) and diarrheagenic E. coli (DEC) by multiplex PCR detection of specific virulence genes. The most prevalent serogroups for all E. coli isolates were O88, O18, O167, O4, and O158. ExPEC isolates were detected mostly in vaginal samples, and DEC isolates were detected only in fecal samples. Phylogenetic group B1 predominated in fecal isolates, while groups B2 and D were frequently detected in vaginal isolates. Resistance to trimethoprim-sulfamethoxazole was most frequently observed, followed by resistance to nalidixic acid and tetracycline. All except five isolates were typeable by using XbaI and were categorized into 74 PFGE patterns. Our findings indicate that panda E. coli isolates exhibited antimicrobial resistance, and potentially pathogenic E. coli isolates were present in giant pandas. In addition, these E. coli isolates were genetically diverse. This study may provide helpful information for developing strategies in the future to control E. coli infections of giant pandas.

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Characterization of atypical Enteropathogenic Escherichia coli (aEPEC) isolated from dogs

Cited by 22 publications

References 26 publications

Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil

Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil

Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran

Serotypes, Virulence Factors, and Antimicrobial Susceptibilities of Vaginal and Fecal Isolates of Escherichia coli from Giant Pandas

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