Characterization of Autoantibodies Generated in Mice by Immunization with the C-Terminal Region ofTrypanosoma cruziRibosomal P1 and P2 Proteins

Motrán, Claudia Cristina; Cerbán, Fabio M.; Rivarola, Héctor Walter; Cima, Elsa Vottero de

doi:10.1006/clim.1998.4678

Cited by 13 publications

(7 citation statements)

References 27 publications

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“…Immunization against T. cruzi has been disregarded as a possible prophylactic measure due to the general low efficiency, induction of immune abnormalities (Pestel et al, 1992;Tarleton et al, 1981) and even heart disease (Motran et al, 1998;Motran et al, 1999;Ruiz et al, 1985) by experimental procedures. On the other hand, some other counter-examples evidence that immunization can be a safe and efficient measure against T. cruzi (Andrews et al, 1985;Lima et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Trypanosoma cruzi: requirements for induction and maintenance of protective immunity conferred by immunization

Paiva¹,

Pyrrho²,

Ribeiro³

et al. 2002

Experimental Parasitology

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Immunization with CL-14-trypomastigotes generates efficient humoral and cellular responses against infective challenge. Herein, we investigated the relevance of these mechanisms in vivo. Immunization with live CL-14-trypomastigotes protected only part of beta2m(-/-) mice but efficiently protected perforin-knockout mice. Fixed CL-14-trypomastigotes could successfully immunize BALB/c, though live trypomastigotes lowered the requirements for doses and time intervals. Post-immune depletion of CD4 or CD8 subsets did not affect protection conferred by immunization, but switched the production of anti-Trypanosoma cruzi antibodies to IgG2a. Sublethal irradiation partially broke the resistance of immune mice, leading to development of late parasitemia. Passive serum transfer from immune mice conferred protection to nai;ve mice. Our results indicate that presentation of cytosolic antigens by MHC class I molecules is involved in the generation of immunity and suggest that the humoral response contributes to a great extent to keep CL-14-immunized mice protected against infective challenge.

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Section: Discussionmentioning

confidence: 99%

Trypanosoma cruzi: requirements for induction and maintenance of protective immunity conferred by immunization

Paiva¹,

Pyrrho²,

Ribeiro³

et al. 2002

Experimental Parasitology

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show abstract

“…One question to be elucidated is whether the presence of the parasite is essential to induce cardiac injury or whether the immune response induced by a single parasite antigen may promote heart damage. Parasite fractions, T. cruzi ribosomal proteins and peptides derived from them have been implicated in such responses [23][24][25].…”

Section: Discussionmentioning

confidence: 99%

Induction of antibodies reactive to cardiac myosin and development of heart alterations in cruzipain-immunized mice and their offspring

et al. 2000

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“…The quantification of IgG1 and IgG2a antibody isotypes against R13, H13 and F105 was carried out as described [24]. Titrations were performed in duplicate and the end point was expressed as the serum dilution, with the optical density reading being twice that of the corresponding NI.…”

Section: Methodsmentioning

confidence: 99%

3-Hydroxy Kynurenine Treatment Controls T. cruzi Replication and the Inflammatory Pathology Preventing the Clinical Symptoms of Chronic Chagas Disease

et al. 2011

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Background3-Hydroxy Kynurenine (3-HK) administration during the acute phase of Trypanosoma. cruzi infection decreases the parasitemia of lethally infected mice and improves their survival. However, due to the fact that the treatment with 3-HK is unable to eradicate the parasite, together with the known proapoptotic and immunoregulatory properties of 3-HK and their downstream catabolites, it is possible that the 3-HK treatment is effective during the acute phase of the infection by controlling the parasite replication, but at the same time suppressed the protective T cell response before pathogen clearance worsening the chronic phase of the infection. Therefore, in the present study, we investigated the effect of 3-HK treatment on the development of chronic Chagas’ disease.Principal FindingsIn the present study, we treated mice infected with T. cruzi with 3-HK at day five post infection during 5 consecutive days and investigated the effect of this treatment on the development of chronic Chagas disease. Cardiac functional (electrocardiogram) and histopathological studies were done at 60 dpi. 3-HK treatment markedly reduced the incidence and the severity of the electrocardiogram alterations and the inflammatory infiltrates and fibrosis in heart and skeletal muscle. 3-HK treatment modulated the immune response at the acute phase of the infection impairing the Th1- and Th2-type specific response and inducing TGF-β-secreting cells promoting the emergence of regulatory T cells and long-term specific IFN-γ secreting cells. 3-HK in vitro induced regulatory phenotype in T cells from T. cruzi acutely infected mice.ConclusionsOur results show that the early 3-HK treatment was effective in reducing the cardiac lesions as well as altering the pattern of the immune response in experimental Chagas’ disease. Thus, we propose 3-HK as a novel therapeutic treatment able to control both the parasite replication and the inflammatory response.

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Characterization of Autoantibodies Generated in Mice by Immunization with the C-Terminal Region ofTrypanosoma cruziRibosomal P1 and P2 Proteins

Cited by 13 publications

References 27 publications

Trypanosoma cruzi: requirements for induction and maintenance of protective immunity conferred by immunization

Trypanosoma cruzi: requirements for induction and maintenance of protective immunity conferred by immunization

Induction of antibodies reactive to cardiac myosin and development of heart alterations in cruzipain-immunized mice and their offspring

3-Hydroxy Kynurenine Treatment Controls T. cruzi Replication and the Inflammatory Pathology Preventing the Clinical Symptoms of Chronic Chagas Disease

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