Characterization of autochthonous lactobacilli from goat dairy products with probiotic potential for metabolic diseases

Effects of Fermented Goat Milk on Adiposity and Gut Microbiota in a Diet-Induced Obesity Murine Model

“…As evidenced by the AI, regular ingestion of the FGM+Mix2 and Mix3 did not alter fat deposits. Our group obtained similar results in a previous study in HFD-induced obese mice, where the BWG of the Ob group was 40% higher than the Control group, and it decreased by 19, 14, and 15% with the administration of CRL1446, CRL1449, and CRL1472, respectively, reaching values similar to the Control group [11].…”

“…In a previous study, we demonstrated the ability of the strains under study to modulate cytokine secretion in macrophages and adipocytes using an in vitro technique, and we observed that these strains decreased the secretion of the proinflammatory TNF-α and IL-6 molecules compared to that triggered by lipopolysaccharide stimuli. And strains CRL1446 and CRL1447 induce greater secretion of the anti-inflammatory IL-10 cytokine values compared to the basal control of production of macrophages [11]. The effect of Lactobacillus strains on the regulation of the immune system has been previously described [25,26].…”

“…indicus CRL1447 (CRL1447), Limosilactobacillus fermentum CRL1446 (CRL1446), Lactiplantibacillus paraplantarum CRL1449 (CRL1449), and Lactiplantibacillus paraplantarum CRL1472 (CRL1472). These strains, previously characterized for their in vitro probiotic and technology properties, were selected to be assayed on diet-induced obese mice [11]. Microorganisms were cultured in de Man-Rogosa-Sharpe (MRS) broth in aerobiosis for 18 h at 37 • C, except for the CRL1447 strain that was cultivated at 42 • C. A combination of these strains was used: Mix1 (CRL1446, CRL1449, and CRL1472), Mix2 (CRL1446 and CRL1449), Mix3 (CRL1446 and CRL1472), and Mix4 (CRL1449 and CRL1472) in a concentration of 1 × 10 8 CFU mL −1 each strain.…”

“…The FGM was elaborated as described by Marquez et al [11]. Briefly, commercial GM powder (La Primera, Córdoba, Argentina) reconstituted in 10% (w/v) sterile distilled water was pasteurized in a thermostatic bath (90 • C for 15 min) and cooled (45 • C).…”

“…Marquez et al, in an intensive study of strain characterization in terms of their probiotic and technological properties, selected Lactobacillus delbrueckii subsp. indicus CRL1447 for developing fermented goat milk [11]. The CRL1447 strain stands out for its high acidification rate in milk, a property of technological interest for developing fermented products.…”