Characterization of Bacillus velezensis RDA1 as a Biological Control Agent against White Root Rot Disease Caused by Rosellinia necatrix

Sawant, Shailesh S.; Song, Jianzhong; Seo, Ho-Jin

doi:10.3390/plants11192486

Cited by 12 publications

(4 citation statements)

References 60 publications

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“…B. subtilis Bs 8B-1 has produced a sufficient amount of antifungal VOCs after 5 days of incubation with Phytophthora capsici , causing cucumber damping-off, and Rhizoctonia solani , causing radish damping-off [ 45 ]. B. velezensis RDA1 VOCs inhibited the growth of Rosellinia necatrix by approximately 60–70% compared to control after 10 days of treatment [ 46 ].…”

Section: Mechanisms Of Action Of Antifungal Bacillus ...mentioning

confidence: 99%

Bacillus VOCs in the Context of Biological Control

2023

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A contemporary agricultural production system relying on heavy usage of agrochemicals represents a questionable outlook for sustainable food supply in the future. The visible negative environmental impacts and unforeseen consequences to human and animal health have been requiring a shift towards the novel eco-friendly alternatives for chemical pesticides for a while now. Microbial-based biocontrol agents have shown a promising potential for plant disease management. The bacteria of the genus Bacillus have been among the most exploited microbial active components due to several highly efficient mechanisms of action against plant pathogens, as well as a palette of additional plant-beneficial mechanisms, together with their suitable properties for microbial biopesticide formulations. Among other bioactive metabolites, volatile organic compounds (VOCs) have been investigated for their biocontrol applications, exhibiting the main advantage of long-distance effect without the necessity for direct contact with plants or pathogens. The aim of this study is to give an overview of the state-of-the-art in the field of Bacillus-based VOCs, especially in terms of their antibacterial, antifungal, and nematicidal action as the main segments determining their potential for biocontrol applications in sustainable agriculture.

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Section: Mechanisms Of Action Of Antifungal Bacillus ...mentioning

confidence: 99%

Bacillus VOCs in the Context of Biological Control

2023

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“…As B. velezensis UTB96 has superior lipopeptide production, it was reasonable to observe enhanced antifungal activity against D. longicolla compared to FZB42 (Figure 4). Previous studies have demonstrated that Bacillus velezensis strains have a broad antagonism activity against several fungal phytopathogens such as soil-borne pathogens Fusarium graminearum, F. solani, F. oxysporum, Rhizoctonia solani, Ralstonia solanacearum, Rosellinia necatrix [55][56][57][58] and the common postharvest pathogens like Botrytis cinerea, Penicillium digitatum and Monilinia fructicola [59][60][61]. More specifically, it is reported that the lipopeptides bacillomycin D and fengycin produced by FZB42 contribute significantly to antifungal activity [62,63].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Bacillus velezensis UTB96, Demonstrating Improved Lipopeptide Production Compared to the Strain B. velezensis FZB42

et al. 2022

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Bacillus strains can produce various lipopeptides, known for their antifungal properties. This makes them attractive metabolites for applications in agriculture. Therefore, identification of productive wild-type strains is essential for the development of biopesticides. Bacillus velezensis FZB42 is a well-established strain for biocontrol of plant pathogens in agriculture. Here, we characterized an alternative strain, B. velezensis UTB96, that can produce higher amounts of all three major lipopeptide families, namely surfactin, fengycin, and iturin. UTB96 produces iturin A. Furthermore, UTB96 showed superior antifungal activity towards the soybean fungal pathogen Diaporthe longicolla compared to FZB42. Moreover, the additional provision of different amino acids for lipopeptide production in UTB96 was investigated. Lysine and alanine had stimulatory effects on the production of all three lipopeptide families, while supplementation of leucine, valine and isoleucine decreased the lipopeptide bioproduction. Using a 45-litre bioreactor system for upscaling in batch culture, lipopeptide titers of about 140 mg/L surfactin, 620 mg/L iturin A, and 45 mg/L fengycin were achieved. In conclusion, it becomes clear that B. velezensis UTB96 is a promising strain for further research application in the field of agricultural biological controls of fungal diseases.

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“…For example, strains of B subtilis, which are abundant in the environment, produce antibiotic lipopeptides, including iturin [74], surfactants [75], and fengycin [76]. Enzymes of the lipopeptide group protect Fusarium oxysporum [75], and Rosellinia necatrix in horticultural crops [78], by inducing systemic resistance in the host plant or suppressing the growth of pathogenic fungi directly [79][80][81]. This shows the potential use of indigenous Bacillus as a plant regulator and biocontrol of potato pathogens.…”

Section: (C))mentioning

confidence: 99%

Distinctive Bacteria Consortium of Rhizosphere and Surface Soil from Semi-Organic Potato Cultivation in Magelang, Indonesia

et al. 2023

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Potato cultivation in the middle land is an alternative solution to avoid pathogens. Although it reduces potato quantity and requires more chemical fertilizers to increase production that potentially damages the environment. Therefore, this study aims to characterize contaminations and indigenous microbes from semi-organic potato cultivation centers in Central Java. Soil samples from the semi-organic potato cultivation center of Kledung, Central, were analyzed for metal contamination using the atomic absorption spectrophotometry method and metagenomic soil bacterial diversity profiles. Specifically targeted genes of distinct regions 16SV3-V5. OTUs were identified using Uparse v7.0.1001, while Beta diversity analysis used UniFrac. All these indices in our samples were calculated with QIIME (Version 1.7.0) and displayed with R software (Version 2.15.3). The results showed that semi-organic cultivations had been polluted with Pb > Cd > Cr > As, and carbamate residue was detected in the rhizosphere and surface soil. Manure and compost application alongside NPK fertilizer increases N input, decreasing surface soil bacteria. The surface soil (with a depth of ≤ 5 cm) has a lower index of species richness, diversity, and relative abundance than the rhizosphere layer (depth > 5 cm). Firmicutes, Proteobacteria, and Actinobacterium dominate soil-level bacteria. In comparison, the rhizosphere soil has a higher bacteria diversity dominated by N-tethering rhizobia from the phylum Proteobacteria. The potato root attracts rhizobacteria consortium that support plant growth. A further study should be conducted to describe how the bacterial consortium formed, rhizobia migration from surface to rhizosphere soil, and its interaction with the potato plants. HIGHLIGHTS Semi-organic potato cultivation in Kledung, Temanggung, Indonesia, is contaminated with heavy metals, especially Pb, which is the highest pollutant, and the carbamate residue Excessive nitrogen (N) input from organic and chemical fertilizers reduces the diversity of soil surface bacteria Then manure application as organic fertilizer introduces pathogenic bacteria such as Eschericia-Shigella, Faecalibaculum, Fusobacteria, Bacilli, and Enterococcus The rhizosphere soil is dominated by N-fixating rhizobia from Proteobacteria, including Pseudoxanthomonas Mexicana, Lysobacteria , Devosia riboflavina, Aminobacter sp., Bosea sp., Allorhizobium, Neorhizobium, Pararhizobium, and Rhizobium GRAPHICAL ABSTRACT

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Characterization of Bacillus velezensis RDA1 as a Biological Control Agent against White Root Rot Disease Caused by Rosellinia necatrix

Cited by 12 publications

References 60 publications

Bacillus VOCs in the Context of Biological Control

Bacillus VOCs in the Context of Biological Control

Characterization of Bacillus velezensis UTB96, Demonstrating Improved Lipopeptide Production Compared to the Strain B. velezensis FZB42

Distinctive Bacteria Consortium of Rhizosphere and Surface Soil from Semi-Organic Potato Cultivation in Magelang, Indonesia

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