Characterization of Bacteria That Cause "Bacterial Shoot Blight of Peard" in Japan

Beer, Steven V.; Kim, J. H.; Zumoff, C. H.; Bogdanove, Adam J.; Laby, Ron J.; Gustafson, H.L.; Momol, Tim; Aldwinckle, Herb S.; Tanii, A.; Tamura, Osamu

doi:10.17660/actahortic.1996.411.36

Cited by 27 publications

(19 citation statements)

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“…The disease symptoms were almost indistinguishable to those of Erwinia amylovora, the causative agent of fire blight disease in pome fruits and variety of Rosaceae species (Beer et al 1996). Because of the similarity in disease symptoms, the pathogens were provisionally described as E. amylovora (Beer et al 1996).…”

Section: Introductionmentioning

confidence: 99%

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Comparative genomics of JapaneseErwinia pyrifoliaestrain Ejp617 with closely related erwinias

Thapa

Park

Kim

et al. 2013

Genome

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Japanese Erwinia pyrifoliae strains cause bacterial shoot blight of pear (BSBP) in Japan. The genetics of Japanese Erwinia remains largely unknown relative to the abundant genomic information available for other Erwinia strains. We compared the genome of Japanese and Korean E. pyrifoliae strains along with those of E. amylovora and E. tasmaniensis. Comparisons with the Korean E. pyrifoliae strain revealed numerous gene insertions/deletions, rearrangements, and inversions in the central regions of the chromosomes. Approximately 80% (2843) of coding DNA sequences (CDSs) are shared by these two genomes which represent about three-quarters of the genome, and there are about 20% unique CDSs. Comparative analysis with closely related erwinias showed that 1942 (more than 50%) core open reading frames (ORF) are shared by all these strains. In addition to two type III secretion systems (hrp/dsp and inv/spa), the genome of Ejp617 encodes numerous virulence factors, including a type VI secretion system, an exopolysaccharide synthesis cluster, and another protein secretion system present in plant pathogenic Erwinia strains. The availability of whole genome sequence should provide a resource to further improve the understanding of pathogenesis in Japanese E. pyrifoliae Ejp617 and to facilitate evolutionary studies among the species of the genus Erwinia.

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Section: Introductionmentioning

confidence: 99%

“…Japanese Erwinia strains causing bacterial shoot blight of pear (BSBP) were first isolated from Nashi pears in Hokkaido, Japan (Beer et al 1996). The disease symptoms were almost indistinguishable to those of Erwinia amylovora, the causative agent of fire blight disease in pome fruits and variety of Rosaceae species (Beer et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Comparative genomics of JapaneseErwinia pyrifoliaestrain Ejp617 with closely related erwinias

Thapa

Park

Kim

et al. 2013

Genome

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“…that causes bacterial shoot blight of pear was reported to be more closely related to E. pyrifoliae than to the typical E. amylovora (Beer et al 1996;Jock et al 2003aJock et al , 2003bKim et al 2001a;McGhee et al 2002). In addition, a recent comparative study using amplified fragment length polymorphism (AFLP) and groEL sequence analysis showed that Erwinia pathogen of pear in Japan is closely related to E. pyrifoliae and is distinct from E. amylovora (Maxson-Stein et al 2003;Mizuno et al 2000).…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, the hrp genes of group II are regulated by HrpX (Xanthomonas species) and HrpB (R. solanacearum) of the members of the AraC-type family of regulators (Wengelnik and Bonas 1996). There are nine highly conserved hrp genes in both plant and animal bacterial pathogens known as the hrc (hrp conserved) genes (Bogdanove et al 1996), and they encode components of the type III secretion system (TTSS). The TTSS mediates translocation of effector proteins across the bacterial membrane and into the host.…”

Section: Introductionmentioning

confidence: 99%

Identification of dspEF, hrpW, and hrpN loci and characterization of the hrpNEp gene in Erwinia pyrifoliae

et al. 2005

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Erwinia pyrifoliae, the causal pathogen of shoot blight in the Asian pear tree (Pyrus pyrifolia cv. Singo), is host-specific and endemic to Korea. To identify the genes associated with the hypersensitive response (HR) and pathogenicity, a genomic library of E. pyrifoliae WT3 was constructed, and the cosmid clone Escherichia coli (pCEP33) was selected. Sequence analysis of 19.7-kb pCEP33 determined disease-specific (dsp) region homolog and approximately 40% of the hrp genes, which included hrpW, hrpN Ep , hrpV, hrpT, hrcC, hrpG, hrpF, and partial hrpE homologs, with respect to the cluster of Erwinia amylovora. Additionally, two open reading frames, ORFD and ORFE, were found downstream of the dspEF region. The results of the sequence analysis showed that the pCEP33 did not contain any hrp regulatory genes or most of the genes encoding components of the Hrp protein secretion system. The hrpN Ep gene of E. pyrifoliae contained five intergenic nucleotide fragment insertions (INFIs) and produced the HR elicitor protein harpin Ep , with a molecular mass of approximately 44 kDa. The purified HrpN Ep protein elicited faster and stronger HR when infiltrated into tobacco leaves than did HrpN Ea from E. amylovora. To observe the role of the hrpL gene in the expression of HrpN Ep , the pEL2 containing hrpL was used to transform E. coli (pCEP33). Expression of HrpN Ep in E. coli (pCEP33 ϩ pEPL2) was detected with an immunoblot using antiserum raised against HrpN Ep , indicating a role of hrpL gene in enhancing the expression of HrpN Ep .

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“…The Japanese Erwinia strain Ejp617, along with three other strains (Ejp556, Ejp557, and Ejp562), was isolated from Nashi pears in Japan (1). These strains cause bacterial shoot blight of pear (BSBP), which has symptoms similar to those of infection with Erwinia amylovora, the causative agent of fire blight (4).…”

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confidence: 99%

Complete Genome Sequence of Japanese Erwinia Strain Ejp617, a Bacterial Shoot Blight Pathogen of Pear

et al. 2011

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Characterization of Bacteria That Cause "Bacterial Shoot Blight of Peard" in Japan

Cited by 27 publications

References 0 publications

Comparative genomics of JapaneseErwinia pyrifoliaestrain Ejp617 with closely related erwinias

Comparative genomics of JapaneseErwinia pyrifoliaestrain Ejp617 with closely related erwinias

Identification of dspEF, hrpW, and hrpN loci and characterization of the hrpNEp gene in Erwinia pyrifoliae

Complete Genome Sequence of Japanese Erwinia Strain Ejp617, a Bacterial Shoot Blight Pathogen of Pear

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