Characterization of Bacterial Cellulose Produced by Komagataeibacter maltaceti P285 Isolated from Contaminated Honey Wine

Abstract

Bacterial cellulose (BC), a biopolymer, is synthesized by BC-producing bacteria. Almost all producing strains are classified in the family Acetobacteraceae. In this study, bacterial strain P285 was isolated from contaminated honey wine in a honey factory in northern Thailand. Based on 16S rRNA gene sequence identification, the strain P285 revealed 99.8% identity with Komagataeibacter mal-taceti LMG 1529 T. K. maltaceti P285 produced the maximum BC production at 20–30 °C and an initial media pH of 9.0. The highest BC production in modified mineral salt medium (MSM) was exhibited when glucose (16%, w/v) and yeast extract (3.2%, w/v) were applied as carbon and nitrogen sources, respectively. When sugarcane (8–16%, w/v) or honey (ratio of honey to water = 1: 4) supplemented with yeast extract was used, the BC production was greater. The characterization of BC synthesized by K. maltaceti P285 was undertaken using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectrometry. Meanwhile, X-ray diffraction results confirmed the presence of crystalline cellulose (2θ = 18.330, 21.390 and 22.640°). The maximum temperature of BC degradation was observed at 314 °C. Tensile properties analysis of hydrated and dried BC showed breaking strength of 1.49 and 0.66 MPa, respectively. These results demonstrated that K. maltaceti P285 has a high potential for BC production especially when grown in high initial media pH. Therefore, the strain would be suitable as an agent to make BC, the value-added product in the related factories.

Nanobacterial Cellulose Production and Its Antibacterial Activity in Biodegradable Poly(vinyl alcohol) Membranes for Food Packaging Applications

“…as shown in Figure . The harvested NBC was found to be thick and was floating on the surface of the media at the air–liquid interface. , Further, the selected strain LRP1 was molecularly characterized based on the 16s rRNA gene for specific primers 907R_704F. LRP1 was identified as Bacillus subtilis by 16s rRNA gene sequencing and a phylogenetic analysis.…”

“…A highly homologous sequence was selected, and a multiple sequence analysis was performed to deduce a phylogenetic tree using the molecular evolutionary genetic analysis (MEGA 6.0) tool. Phylogenetic analysis was used to characterize the isolated strain LRP1 based on the sequence similarity …”

Determination the optimal conditions for production of nanocellulose from acetic acid bacteria

“…Bacterial isolates were recognized by observation of the morphology of its colony, Gram reaction, series and motility tests. The morphology of its cell was examined by using SEM, for identification by biochemical procedure, an estimation of different experiments involving: catalase, oxidase production and fermentation various carbohydrates (Thongwai et al, 2022).…”

“…The BC produced by acetic acid bacteria known as generally, recognized, as safe (GRAS) food by US Food and Drug Administration (FDA) (Thongwai et al, 2022). In this study, the bacterial isolates that have the ability to produce bacterial nanocellulose (BNC) were detected after the incubation at pH (6.5-7), 30°C, inoculum size 2% and incubation period (7-8) days in HS medium, the positive results was observed as a whit thick membrane layer at the surface of the liquid medium with diameter of about 48 mm approximately (Figure 1), the bacterial isolate (acetic acid bacteria 5AC) was selected as best BNC producer after screening.…”

“…Figure (1) Bacterial nanocellulose membrane (wet membrane) produced by acetic acid bacteria in Hestrin-Schramm (HS) mediumHestrin-Schramm broth is favorable medium to get high productivity of BC from AAB Thongwai et al (2022). reported that HS broth pH (6.0), inoculated by 5% (v/v) from the strain Komagataeibacter maltaceti P285 then incubated at temperature 30°C for about 7 day, after cultivation, cellulose harvested then purified with high productivity.…”