Characterization of bacterial communities associated with the pine sawyer beetle<i>Monochamus galloprovincialis</i>, the insect vector of the pinewood nematode<i>Bursaphelenchus xylophilus</i>

Vicente, Cláudia S. L.; Nascimento, Francisco X.; Espada, Margarida; Barbosa, Pedro; Hasegawa, Koichi; Mota, Manuel; Oliveira, Solange

doi:10.1111/1574-6968.12232

Cited by 31 publications

(49 citation statements)

References 52 publications

(60 reference statements)

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“…The percent abundance varied with 89% Proteobacteria, 9% Actinobacteria, and only 1% Firmicute s , which is similar to the pattern of phyla prevalence observed in the microbiome of A. maculatum ticks obtained from the same geographical region (Budachetri et al, 2014). The presence of dominant species belonging to the phylum Proteobacteria in the microbiota of invertebrates has been widely reported (Arias-Cordero et al, 2012; Vicente et al, 2013) and is further supported by our observation of only Proteobacteria in the tested tick midgut samples.…”

Section: Resultssupporting

confidence: 89%

A snapshot of the microbiome of Amblyomma tuberculatum ticks infesting the gopher tortoise, an endangered species

Budachetri

Gaillard

Williams

et al. 2016

Ticks and Tick-borne Diseases

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The gopher tortoise tick, Amblyomma tuberculatum, has a unique relationship with the gopher tortoise, Gopherus polyphemus, found in sandy habitats across the southeastern United States. We aimed to understand the overall bacterial community associated with A. tuberculatum while also focusing on spotted fever group Rickettsia. These tortoises in the Southern Mississippi region are a federally threatened species; therefore, we have carefully trapped the tortoises and removed the species-specific ticks attached to them. Genomic DNA was extracted from individual ticks and used to explore overall bacterial load using pyrosequencing of bacterial 16S rRNA on 454-sequencing platform. The spotted fever group of Rickettsia was explored by amplifying rickettsial outer membrane protein A (rompA) gene by nested PCR. Sequencing results revealed 330 bacterial operational taxonomic units (OTUs) after all the necessary curation of sequences. Four whole A. tuberculatum ticks showed Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes as the most dominant phyla with a total of 74 different bacterial genera detected. Together Rickettsiae and Francisella showed >85% abundance, thus dominating the bacterial community structure. Partial sequences obtained from ompA amplicons revealed the presence of an uncharacterized Rickettsia similar to the Rickettsial endosymbiont of A. tuberculatum. This is the first preliminary profile of a complete bacterial community from gopher tortoise ticks and warrants further investigation regarding the functional role of Rickettsial and Francisella-like endosymbionts in tick physiology.

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Section: Resultssupporting

confidence: 89%

A snapshot of the microbiome of Amblyomma tuberculatum ticks infesting the gopher tortoise, an endangered species

Budachetri

Gaillard

Williams

et al. 2016

Ticks and Tick-borne Diseases

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“…Indeed, all beetles compared in this study belong to the Curculionidae family and it is plausible that the shared microbiota found in these insects reflects their relatedness. However, outside this beetle family, a similar bacterial community has been described in a species of the Cerambycidae (Monochamus galloprovincialis) sampled in Portugal (Vicente et al 2013), which also lives on conifers (i.e. Pinus pinaster).…”

Section: Discussionmentioning

confidence: 63%

“…However, outside this beetle family, a similar bacterial community has been described in a species of the Cerambycidae ( Monochamus galloprovincialis ) sampled in Portugal (Vicente et al . ), which also lives on conifers (i.e. Pinus pinaster ).…”

Section: Discussionmentioning

confidence: 99%

The gut microbiota of the pine weevil is similar across Europe and resembles that of other conifer‐feeding beetles

et al. 2016

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The pine weevil (Hylobius abietis, Coleoptera: Curculionidae) is an important pest of conifer seedlings in Europe. Despite its economic importance, little is known about the composition of its gut microbial community and the role it plays in mediating the weevil's ability to utilize conifers as a food source. Here, we characterized the gut bacterial communities of different populations of H. abietis across Europe and compared them to those of other beetles that occupy similar ecological niches. We demonstrate that the microbial community of H. abietis is similar at higher taxonomic levels (family and genus) across locations in Europe, with Wolbachia as the dominant microbe, followed by Enterobacteria and Firmicutes. Despite this similarity, we observed consistent differences between countries and locations, but not sexes. Our meta-analysis demonstrates that the gut bacterial community of the pine weevil is very similar to that of bark beetles that also exploit conifers as a food source. The Enterobacteriaceae symbionts of both host taxa are especially closely related phylogenetically. Conversely, the microbiota of H. abietis is distinct from that of closely related weevils feeding on nonconifer food sources, suggesting that the microbial community of the pine weevil is determined by the environment and may be relevant to host ecology. Furthermore, several H. abietis-associated members of the Enterobacteriaceae family are known to contain genes involved in terpenoid degradation. As such, we hypothesize that the gut microbial community is important for the utilization of conifer seedlings as a food source, either through the detoxification of plant secondary metabolites or through the supplementation of essential nutrients.

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“…In the insect vector Monochamus galloprovincialis (nematode free), only seven bacterial genera were specifically identified, namely, Lysinibacillus , Lactococcus , Morganella , Providencia , Photorhabdus and Sediminibacterium (Vicente et al ., ). In Bursaphelenchus mucronatus (obtained from symptomatic trees), only three particular species were unique of this nematode, namely Pseudomonas aeruginosa , Escherichia intermedius and Staphylococcus auriculans (Zhao et al ., ; Tian et al ., ).…”

Section: Bacterial Communities Associated With the Pwn And Its Insectmentioning

confidence: 97%

Bacterial role in pine wilt disease development – review and future perspectives

Nascimento

Hasegawa

Mota

et al. 2014

Environ Microbiol Rep

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Mutualistic and beneficial relationships between nematodes and bacteria are highly present in nature, mostly occurring because of nutritional dependence and pathogen protection, and intrinsically related with the environment, the ecological conditions and the nematode life stages. Thirty-four years have passed since the first hypothesis suggesting a bacterial role in pine wilt disease (PWD), associated with the pinewood nematode (PWN), Bursaphelenchus xylophilus. In 1980, researchers reported that bacteria associated with the PWN could produce toxins that lead to PWD development in pine seedlings. It was also suggested a double vector system for PWD, where bacteria were vectored by the PWN and the PWN vectored by an insect from the Monochamus genus. Presently, the specific involvement of bacteria in such complex disease is still controversial, even though the increased number of studies focused on the potential bacteria role has increased considerably. This review is an up-to-date comprehensive perspective and brings new insights on the role of PWN-associated bacteria in PWD.

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Characterization of bacterial communities associated with the pine sawyer beetleMonochamus galloprovincialis, the insect vector of the pinewood nematodeBursaphelenchus xylophilus

Cited by 31 publications

References 52 publications

A snapshot of the microbiome of Amblyomma tuberculatum ticks infesting the gopher tortoise, an endangered species

A snapshot of the microbiome of Amblyomma tuberculatum ticks infesting the gopher tortoise, an endangered species

The gut microbiota of the pine weevil is similar across Europe and resembles that of other conifer‐feeding beetles

Bacterial role in pine wilt disease development – review and future perspectives

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