Characterization of BASP1‐mediated neurite outgrowth

Korshunova, Irina; Caroni, Pico; Kolkova, Kateryna; Berezin, Vladimir; Bock, Elisabeth; Walmod, Peter S.

doi:10.1002/jnr.21678

Cited by 74 publications

(73 citation statements)

References 54 publications

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“…The data here indicate a significant reduction in brain acid soluble protein 1 (BASP1) in the cortex following cuprizone treatment. As BASP1 plays a role in neurite growth [29], its loss may potentially be related to axonal damage that has been reported in this model as well as the later stages of clinical MS. Furthermore, neurocalcin was no longer detected in the cortex proteome following cuprizone treatment.…”

Section: Discussionmentioning

confidence: 63%

An initial top-down proteomic analysis of the standard cuprizone mouse model of multiple sclerosis

et al. 2015

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An initial proteomic analysis of the cuprizone mouse model to characterise the breadth of toxicity by assessing cortex, skeletal muscle, spleen and peripheral blood mononuclear cells. Cuprizone treated vs. control mice for an initial characterisation. Select tissues from each group were pooled, analysed in triplicate using two-dimensional gel electrophoresis (2DE) and deep imaging and altered protein species identified using liquid chromatography tandem mass spectrometry (LC/MS/MS). Forty-three proteins were found to be uniquely detectable or undetectable in the cuprizone treatment group across the tissues analysed. Protein species identified in the cortex may potentially be linked to axonal damage in this model, and those in the spleen and peripheral blood mononuclear cells to the minimal peripheral immune cell infiltration into the central nervous system during cuprizone mediated demyelination. Primary oligodendrocytosis has been observed in type III lesions in multiple sclerosis. However, the underlying mechanisms are poorly understood. Cuprizone treatment results in oligodendrocyte apoptosis and secondary demyelination. This initial analysis identified proteins likely related to axonal damage; these may link primary oligodendrocytosis and secondary axonal damage. Furthermore, this appears to be the first study of the cuprizone model to also identify alterations in the proteomes of skeletal muscle, spleen and peripheral blood mononuclear cells. Notably, protein disulphide isomerase was not detected in the cuprizone cohort; its absence has been linked to reduced major histocompatibility class I assembly and reduced antigen presentation. Overall, the results suggest that, like experimental autoimmune encephalomyelitis, results from the standard cuprizone model should be carefully considered relative to clinical multiple sclerosis.

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Section: Discussionmentioning

confidence: 63%

An initial top-down proteomic analysis of the standard cuprizone mouse model of multiple sclerosis

et al. 2015

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“…In this study, we identified three novel miR-7 target genes that are upregulated in gastric cancers in an inflammation-dependent manner. LPHN2 is a G protein-coupled receptor that binds a-latrotoxin (Ichtchenko et al, 1999), whereas BASP1 is implicated in neurite outgrowth (Korshunova et al, 2008). MAFG is one of the small Maf proteins that is important for antioxidant responses (Katsuoka et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Inflammation-induced repression of tumor suppressor miR-7 in gastric tumor cells

Kong

Piao

Yamashita³

et al. 2011

Oncogene

102

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Inflammation has an important role in cancer development through various mechanisms. It has been shown that dysregulation of microRNAs (miRNAs) that function as oncogenes or tumor suppressors contributes to tumorigenesis. However, the relationship between inflammation and cancer-related miRNA expression in tumorigenesis has not yet been fully understood. Using K19-C2mE and Gan mouse models that develop gastritis and gastritis-associated tumors, respectively, we found that 21 miRNAs were upregulated, and that 29 miRNAs were downregulated in gastric tumors in an inflammation-dependent manner. Among these miRNAs, the expression of miR-7, a possible tumor suppressor, significantly decreased in both gastritis and gastric tumors. Moreover, the expression of miR-7 in human gastric cancer was inversely correlated with the levels of interleukin-1b and tumor necrosis factor-a, suggesting that miR-7 downregulation is related to the severity of inflammatory responses. In the normal mouse stomach, miR-7 expression was at a basal level in undifferentiated gastric epithelial cells, and was induced during differentiation. Moreover, transfection of a miR-7 precursor into gastric cancer cells suppressed cell proliferation and soft agar colony formation. These results suggest that suppression of miR-7 expression is important for maintaining the undifferentiated status of gastric epithelial cells, and thus contributes to gastric tumorigenesis. Although epigenetic changes were not found in the CpG islands around miR-7-1 of gastritis and gastric tumor cells, we found that activated macrophage-derived small molecule(s) (o3 kDa) are responsible for miR-7 repression in gastric cancer cells. Furthermore, the miR-7 expression level significantly decreased in the inflamed gastric mucosa of Helicobacter-infected mice, whereas it increased in the stomach of germfree K19-C2mE and Gan mice wherein inflammatory responses were suppressed.Taken together, these results indicate that downregulation of tumor suppressor miR-7 is a novel mechanism by which the inflammatory response promotes gastric tumorigenesis.

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“…BASP1 and VIME are proteins implicated in the maintenance of the cell integrity and the stabilisation of the cytoskeletal interactions (Goldman et al 1996, Korshunova et al 2008; as such, they play roles in adhesion, migration, survival and cell signalling. HNRPC is a nuclear pre-mRNA binding protein that has also been implicated in cell proliferation (Kim et al 2003, Schepens et al 2007) and DPY30 is a protein involved in differentiation (Jiang et al 2011).…”

Section: Proliferation and Differentiationmentioning

confidence: 99%

The effect of pre-existing maternal obesity on the placental proteome: two-dimensional difference gel electrophoresis coupled with mass spectrometry

Oliva¹,

Barker²,

Riley³

et al. 2012

Journal of Molecular Endocrinology

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Our aim was to study the protein expression profiles of placenta obtained from lean and obese pregnant women with normal glucose tolerance at the time of term Caesarean section. We used two-dimensional difference gel electrophoresis (2D-DIGE), utilising narrow-range immobilised pH gradient strips that encompassed the broad pH range of 4–5 and 5–6, followed by MALDI-TOF mass spectrometry of selected protein spots. Western blot and quantitative RT-PCR (qRT-PCR) analyses were performed to validate representative findings from the 2D-DIGE analysis. Eight proteins were altered (six down-regulated and two up-regulated on obese placentas). Annexin A5 (ANXA5), ATP synthase subunit beta, mitochondria (ATPB), brain acid soluble protein 1 (BASP1), ferritin light chain (FTL), heterogeneous nuclear ribonucleoprotein C (HNRPC) and vimentin (VIME) were all lower in obese patients. Alpha-1-antitrypsin (A1AT) and stress-70 protein, mitochondrial (GRP75) were higher in obese patients. Western blot analysis of ANXA5, ATPB, FTL, VIME, A1AT and GRP75 confirmed the findings from the 2D-DIGE analysis. For brain acid soluble protein 1 and HNRPC, qRT-PCR analysis also confirmed the findings from the 2D-DIGE analysis. Immunohistochemical analysis was also used to determine the localisation of the proteins in human placenta. In conclusion, proteomic analysis of placenta reveals differential expression of several proteins in patients with pre-existing obesity. These proteins are implicated in a variety of cellular functions such as regulation of growth, cytoskeletal structure, oxidative stress, inflammation, coagulation and apoptosis. These disturbances may have significant implications for fetal growth and development.

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Characterization of BASP1‐mediated neurite outgrowth

Cited by 74 publications

References 54 publications

An initial top-down proteomic analysis of the standard cuprizone mouse model of multiple sclerosis

An initial top-down proteomic analysis of the standard cuprizone mouse model of multiple sclerosis

Inflammation-induced repression of tumor suppressor miR-7 in gastric tumor cells

The effect of pre-existing maternal obesity on the placental proteome: two-dimensional difference gel electrophoresis coupled with mass spectrometry

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