Characterization of beta-lactamases from non-Bacteroides fragilis group Bacteroides spp. belonging to seven species and their role in beta-lactam resistance

Appelbaum, Peter C.; Philippon, A.; Jacobs, M. R.; Spangler, S K; Gutmann, Laurent

doi:10.1128/aac.34.11.2169

Cited by 43 publications

(31 citation statements)

References 53 publications

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“…␤-lactamase production is found in most of the Bacteroides fragilis group and has increasingly been found in Prevotella, Porphyromonas, and Fusobacterium spp. Clindamycin resistance is found in the B. fragilis group and some Clostridium strains, and metronidazole resistance, common among anaerobic gram-positive non-sporeforming rods, has also been found in the B. fragilis group (1)(2)(3)(4).…”

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confidence: 99%

Antianaerobe Activity of RBX 7644 (Ranbezolid), a New Oxazolidinone, Compared with Those of Eight Other Agents

Ednie

Rattan²,

Jacobs

et al. 2003

Antimicrob Agents Chemother

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The activity of ranbezolid (RBX 7644), a new oxazolidinone, against 306 anaerobes was compared with those of 11 other agents. The MICs at which 50% of the isolates tested are inhibited and those at which 90% of the isolates tested are inhibited (in micrograms per milliliter) were as follows: ranbezolid, 0.03 and 0.5; linezolid, 2 and 4; vancomycin, >16 and >16; teicoplanin, 1 and >16; quinupristin-dalfopristin, 1 and >8; amoxicillinclavulanate, 0.5 and 2; imipenem, 0.125 and 1; clindamycin, 0.25 and 8; metronidazole, 1 and 4; gatifloxacin, 0.5 and 4; and moxifloxacin, 0.5 and 2, respectively. Ranbezolid had very good in vitro activity against both gram-negative and -positive anaerobes.Anaerobes are frequent causes of human infections, e.g., intra-abdominal infections, especially in immunocompromised and otherwise debilitated hosts. ␤-lactamase production is found in most of the Bacteroides fragilis group and has increasingly been found in Prevotella, Porphyromonas, and Fusobacterium spp. Clindamycin resistance is found in the B. fragilis group and some Clostridium strains, and metronidazole resistance, common among anaerobic gram-positive non-sporeforming rods, has also been found in the B. fragilis group (1-4).Oxazolidinones are a new class of synthetic antimicrobial agents active mainly against gram-positive organisms, including gram-positive anaerobes such as Clostridium spp., Peptostreptococcus spp., and Propionibacterium acnes. Linezolid, the oxazolidinone for which most data are currently available, is active against staphylococci, streptococci, enterococci, and aerobic gram-positive non-spore-forming rods as well as grampositive anaerobes. Activity against gram-negative organisms is less marked. Similar MICs have been described for AZD2563, another experimental oxazolidinone (5,7,8,10,13).Ranbezolid (RBX 7644; Ranbaxy Research Laboratories, New Delhi, India) (Fig. 1) is a new oxazolidinone with enhanced activity against gram-positive organisms and activity against some fastidious gram-negative organisms. The present study examines the in vitro activity of ranbezolid in comparison with those of linezolid, vancomycin, teicoplanin, quinupristindalfopristin, amoxicillin, amoxicillin-clavulanate, imipenem, clindamycin, metronidazole, gatifloxacin, and moxifloxacin against 306 gram-positive and gram-negative anaerobes.The strains were all clinical isolates, most of which were isolated during the past 3 years, mostly from the Hershey Medical Center, Hershey, Pa., but also from other hospitals, and were identified by conventional methodology (12). Each Clostridium difficile isolate was from a separate patient, with some patients from the Hershey Medical Center and others from different hospitals. The possibility of clonality (leading to similar susceptibilities) cannot be excluded in all cases. Prior to testing, organisms were stored in double-strength skim milk (Difco Laboratories, Detroit, Mich.) at Ϫ70°C. Purity throughout the study was checked by Gram stain and colonial morphology. Ranbezolid powder was obtai...

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confidence: 99%

Antianaerobe Activity of RBX 7644 (Ranbezolid), a New Oxazolidinone, Compared with Those of Eight Other Agents

Ednie

Rattan²,

Jacobs

et al. 2003

Antimicrob Agents Chemother

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“…␤-Lactamase production also has been described in clostridia. Metronidazole resistance in organisms other than non-spore-forming gram-positive bacilli has been described, as has clindamycin resistance in anaerobic gramnegative bacilli (1)(2)(3)(4)(5).…”

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Antianaerobic Activity of Sulopenem Compared to Six Other Agents

Ednie

Appelbaum

2009

Antimicrob Agents Chemother

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“…Metronidazole resistance in organisms other than non-sporeforming gram-positive rods has been described previously, as has clindamycin resistance in anaerobic gram-negative rods (1)(2)(3)(4)(5).…”

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Activity of CP 99,219 compared with those of ciprofloxacin, grepafloxacin, metronidazole, cefoxitin, piperacillin, and piperacillin-tazobactam against 489 anaerobes

Spangler

Jacobs

Appelbaum

1994

Antimicrob Agents Chemother

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Agar dilution was used to compare the in vitro activity of CP 99,219 with those of ciprofloxacin, grepafloxacin, metronidazole, cefoxitin, piperacillin, and piperacillin-tazobactam against 489 anaerobes. CP 99,219 yielded a MIC for 50%6 of the strains tested (MIC..) of 0.25 ,ug/ml and a MIC90 of 1.0 pg/ml, with 99.6% of the strains susceptible at a breakpoint of 2.0 ,ug/ml. Ciprofloxacin and grepafloxacin were less active (MIC50, 4.0 ,ug/ml; MIC90, 32.0 ,ug/ml and 2.0 and 16.0 ,ug/ml, respectively). Metronidazole was active against all gram-negative rods (MIC%0, 4.0 ,ug/ml), but 31% of the gram-positive anaerobes were resistant at >8.0 ,ug/ml. Cefoxitin was active against 84% of all strains at <16.0 ,g/mli, with a MIC50 of 4.0 ,ug/ml and a MIC90 of 32.0 ,ug/ml. Tazobactam enhanced the activity of piperacillin against >95% of the j-lactamase-producing gram-negative anaerobic rods (MIC90, 16.0 ,ug/ml).Anaerobes are becoming increasingly resistant to 13-lactams because of P-lactamase production and other mechanisms.Metronidazole resistance in organisms other than non-sporeforming gram-positive rods has been described previously, as has clindamycin resistance in anaerobic gram-negative rodsCommercially available quinolones, such as ciprofloxacin, ofloxacin, fleroxacin, pefloxacin, enoxacin, and lomefloxacin, are inactive or marginally active against anaerobes, with MICs either higher than or clustering around breakpoints. Experimental quinolones with increased anti-anaerobic activity include (i) those with slightly increased activity (sparfloxacin, OPC 17116 [grepafloxacin], tosufloxacin, temafloxacin [now discontinued], CI-990, AM-1155, and levofloxacin) and (ii) those with significantly improved anti-anaerobic activity (Win 57273, Bay y3118, clinafloxacin, and DU-6859a) (6-8, 12, 19, 24, 25). Development of both Win 57273 and Bay y3118 has been discontinued because of toxicity.CP 99,219 is a novel investigational trifluoronaphthyridone with a structure differing from those of norfloxacin, ciprofloxacin, and enoxacin. It contains the C-7 moiety 7-(3-azabicyclo [3.1.0.]hexyl) on a basic naphthyridone configuration (9). The bicyclo C-7 substitution has been previously evaluated and described for 667 (17). The 1-N substitution of CP 99,219 is a difluorinated structure identical to that of tosufloxacin that produces enhanced activity against some ciprofloxacinresistant strains. CP 99,219 possesses a broad spectrum of activity against gram-positive and -negative organisms, including those resistant to ciprofloxacin (9,10,13,14,18,21

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Characterization of beta-lactamases from non-Bacteroides fragilis group Bacteroides spp. belonging to seven species and their role in beta-lactam resistance

Cited by 43 publications

References 53 publications

Antianaerobe Activity of RBX 7644 (Ranbezolid), a New Oxazolidinone, Compared with Those of Eight Other Agents

Antianaerobe Activity of RBX 7644 (Ranbezolid), a New Oxazolidinone, Compared with Those of Eight Other Agents

Antianaerobic Activity of Sulopenem Compared to Six Other Agents

Activity of CP 99,219 compared with those of ciprofloxacin, grepafloxacin, metronidazole, cefoxitin, piperacillin, and piperacillin-tazobactam against 489 anaerobes

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