Characterization of Bifidobacterium apousia sp. nov., Bifidobacterium choladohabitans sp. nov., and Bifidobacterium polysaccharolyticum sp. nov., three novel species of the genus Bifidobacterium from honey bee gut

Chen, Jieteng; Wang, Jieni; Zheng, Hao

doi:10.1016/j.syapm.2021.126247

“…Besides, Bifidobacterium spp. significantly contributes to the honey bee's metabolism and health (Chen et al, 2021). Therefore, we presume that Lactobacillus and Bifidobacterium still play important roles in the protection and metabolism of V. velutina.…”

Section: Discussionmentioning

confidence: 93%

Midgut Bacterial Communities of Vespa velutina Lepeletier (Hymenoptera: Vespidae)

Zhang

¹

,

Liu²,

Wang

³

et al. 2022

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Vespa velutina nigrithorax and Vespa velutina auraria are two subspecies of Vespa velutina Lepeletier. V. velutina preys managed honey bees, other pollinators, and insects. However, the Vespa midgut microbiota of three forms, namely queens, workers, and males have not been reported, thus the objective of this study was to analyze the midgut bacterial diversity of the three forms of V. v. nigrithorax and V. v. auraria. Our results showed that Proteobacteria, Firmicutes, Bacteroidetes, Tenericutes, and Actinobacteria were the most abundant phyla, and Lactobacillus (17.21%) and Sphingomonas (11.39%) were the most abundant genera in the midgut of V. v. nigrithorax and V. v. auraria. We found that the midgut bacterial compositions of the V. velutina males were special, in terms of richness and diversity of bacterial communities, as well as the content of lactic acid bacteria. By comparing the gut bacterial compositions of Vespa from different regions (Japan, South Korea, Italy, and China), it was discovered that the gut bacterial compositions were very similar at the phylum and class level, and Gammaproteobacteria, Bacilli, and Alphaproteobacteria were the most abundant classes of bacteria and consistent in the genus Vespa. Besides, though Vespa from different regions had quite different gut bacterial communities at the genus level, Lactobacillus and other lactic acid bacteria were abundant and played important roles in protection and metabolism in V. velutina collected from different regions. This is the first report of midgut bacterial diversity of three forms queens, workers, and males of V. velutina. Our findings provide insight that Proteobacteria and Firmicutes (especially Lactobacillus and other lactic acid bacteria) are consistent and may play important roles in the genus Vespa. The understanding of the microbiome in the midgut of Vespa and the discovery of the vital bacteria would provide useful information to design pest biological control agents. Thus, the significance of this study is to provide a basis for the study of the relationship between gut microbiota and physiology and health of Vespa, as well as the control of Vespa.

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“…Recently, a total of 13 novel species ( Bifidobacterium apousia , Bifidobacterium choladohabitans , Bifidobacterium polysaccharolyticum , Bifidobacterium phasiani , Bifidobacterium pongonis , Bifidobacterium saguinibicoloris , Bifidobacterium colobi , Bifidobacterium simiiventris , Bifidobacterium santillanense , Bifidobacterium miconis , Bifidobacterium amazonense , Bifidobacterium pluvialisilvae and Bifidobacterium miconisargentati ) have been effectively published in Systematic and Applied Microbiology [5, 6]. At the time of writing this paper, B.…”

Section: Data Summarymentioning

confidence: 99%

“…and Bifidobacterium miconisargentati) have been effectively published in Systematic and Applied Microbiology [5,6]. At the time of writing this paper, B. apousia, B. choladohabitans and B. polysaccharolyticum have already been validated and other ten species have not yet been validated.…”

mentioning

confidence: 99%

“…The B. asteroides group includes B. asteroides, Bifidobacterium indicum, Bifidobacterium actinocoloniiforme, Bifidobacterium xylocopae and Bifidobacterium coryneforme [6]. Three recently described species, B. apousia, B. choladohabitans and B. polysaccharolyticum, also belongs to the B. asteroides group [5].…”

mentioning

confidence: 99%

“…+, Positive; -, negative; w, weakly positive; nd, no data. Data for 1-3 from this study; data for 4-7 from[5,34].Fifteen housekeeping gene sequences (TableS5), which are often used in the classification of bifidobacteria[17][18][19][20][21][22][23], were obtained from whole genome sequences by sequence alignment. Results of all housekeeping gene sequence analyses (Figs S1-S15; TableS2) indicated that strain S053-2 T was closely related to the type strains of B. asteroides, B. apousia, B. choladohabitans and B. polysaccharolyticum.…”

mentioning

confidence: 99%

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Bifidobacterium mizhiense sp. nov., isolated from the gut of honeybee (Apis mellifera)

Li

¹

,

Zhang

²

,

Gu

³

2022

International Journal of Systematic and Evolutionary Microbiology

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A novel bifidobacteria (designated S053-2T) was isolated from the gut of honeybee (Apis mellifera). Strain S053-2T was characterized using a polyphasic taxonomic approach. The result of 16S rRNA gene sequence analysis indicated that strain S053-2T was phylogenetically related to the type strains of Bifidobacterium asteroides , Bifidobacterium indicum , Bifidobacterium actinocoloniiforme , Bifidobacterium xylocopae , Bifidobacterium coryneforme , Bifidobacterium apousia , Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum , and had 95.5–99.7 % 16S rRNA gene sequence similarities. Based on the 16S rRNA gene sequence analysis, strain S053-2T was most closely related to the type strain of B. asteroides , having 99.7 % 16S rRNA gene sequence similarity. Strain S053-2T had relatively low (91.6–95.7 %) pheS, atpA, clpC, dnaG, fusA, glnA, glyS, hsp60, argS, pyrG and recA sequence similarities to the type strain of B. asteroides . Strain S053-2T had 94.5–95.3% atpA, clpC, dnaG, dnaK and pyrG sequence similarities to the type strain of B. apousia . The phylogenomic tree indicated that strain S053-2T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides , B. apousia , B. choladohabitans and B. polysaccharolyticum , and distantly related to type strains of other phylogenetically related species in the B. asteroides group. Strain S053-2T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA–DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102T. Strain S053-2T shared 91.1 % ANI, 41.9 % dDDH and 92.5 % AAI values with B. asteroides DSM 20089T. Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and α-fucosidase could differentiate strain S053-2T from B. asteroides DSM 20089T. Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α-fucosidase could differentiate strain S053-2T from B. apousia W8102T. Based upon the data obtained in the present study, a novel species, Bifidobacterium mizhiense sp. nov., is proposed, and the type strain is S053-2T (=JCM 34710T=CCTCC AB 2021129T).

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2024

Implementation of Enzymatic Processes and Lactic Bacteria in the Food Industries

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Characterization of Bifidobacterium apousia sp. nov., Bifidobacterium choladohabitans sp. nov., and Bifidobacterium polysaccharolyticum sp. nov., three novel species of the genus Bifidobacterium from honey bee gut

Cited by 33 publications

References 38 publications

Midgut Bacterial Communities of Vespa velutina Lepeletier (Hymenoptera: Vespidae)

Midgut Bacterial Communities of Vespa velutina Lepeletier (Hymenoptera: Vespidae)

Bifidobacterium mizhiense sp. nov., isolated from the gut of honeybee (Apis mellifera)

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