Characterization of biofilm formation and reduction of hexavalent chromium by bacteria isolated from tannery sludge

Maurya, Annapurna; Kumar, P. Senthil; Raj, Abhay

doi:10.1016/j.chemosphere.2021.131795

Cited by 25 publications

(11 citation statements)

References 48 publications

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“…As mentioned above, the normal plot also confirms that the greatest influence on the formation of biofilms was temperature. Previously conducted studies indicate that pH plays a fundamental role in the formation of bacilli biofilms [33]. Similar to our results, pH 7.0 was also recently reported to be optimal for B. vallismortis biofilm formation [33].…”

Section: Biofilm Formation Studysupporting

confidence: 92%

“…Previously conducted studies indicate that pH plays a fundamental role in the formation of bacilli biofilms [33]. Similar to our results, pH 7.0 was also recently reported to be optimal for B. vallismortis biofilm formation [33]. Regarding the temperature factor, similar to our result, in a recent work by Kilic and Cihan [34], thermophilic B. pumilus also formed optimum biofilm at 50 • C with the highest attachment of sessile cells to multi-well polystyrene plates.…”

Section: Biofilm Formation Studysupporting

confidence: 92%

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Stress Dependent Biofilm Formation and Bioactive Melanin Pigment Production by a Thermophilic Bacillus Species from Chilean Hot Spring

et al. 2022

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Thermophilic bacteria able to survive extreme temperature stress are of great biotechnological interest due to their extracellular production of bioactive molecules as a part of a survival strategy, or by intracellular modifications. In the present study, thermophilic Bacillus haynesii CamB6, isolated from a Chilean hot spring, was studied for the formation of different stress response molecules. The polymeric pigment produced by the bacterial strain was characterized by different physicochemical techniques. On exposure to ranges of temperature (50–60 °C), pH (5.0–7.0), and sources of nitrogen and carbon (1–5 g·L−1), the bacteria responded with a biofilm network formation in a hydrophobic polystyrene surface. Biofilm formation under fed-batch conditions was also statistically validated. The bacteria showed a planktonic pellicle network formation in the presence of induced hypoxia and salinity stress (19.45 g·L−1) under static conditions. Salinity stress also resulted in the intracellular response of brown pigment production. The pigment was structurally and functionally characterized by UV-Vis absorbance and the presence of different characteristic peaks via FTIR analysis (bacterial pyomelanin fingerprints) were assessed. A high thermal stability and TGA profile indicated the brown pigment was a probable pyomelanin candidate. Micropyrolysis (Py-GC/MS) showed that isoprene, pyrrole, benzene, pyridine, and their derivatives were the major components detected. In addition, acetic acid, indole, phenol, and its derivatives were observed. The absence of sulfocompounds in the pyrolyzed products agreed with those reported in the literature for pyomelanin. The pigment surface morphology was analyzed via SEM, and the elemental composition via EDS also demonstrated the similarity of the brown pigment to that of the melanin family. The pyomelanin pigment was observed to be bioactive with promising antioxidant capacity (H2O2, Fe2+) compared to the standard antioxidant molecules. In conclusion, B. haynesii CamB6 demonstrated the formation of several biomolecules as a stress response mechanism that is bioactive, showing its probable biotechnological applications in future.

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Section: Biofilm Formation Studysupporting

confidence: 92%

Section: Biofilm Formation Studysupporting

confidence: 92%

Stress Dependent Biofilm Formation and Bioactive Melanin Pigment Production by a Thermophilic Bacillus Species from Chilean Hot Spring

et al. 2022

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“…PSIM biofilm biomass (cells/mL) the authors have reported immobilization and absorption of heavy metals by the EPS of Bacilli biofilm matrix as the principle behind this process of bioremediation [27,30]. The tolerance of Gram-negative bacteria Rhizobium toward Cr and Cd showed similar results as of our study where the microbes were able to tolerate the concentrations of 0.1 mg/mL and 1 mg/mL of heavy metals [31].…”

Section: Od At a 600 Psimsupporting

confidence: 82%

Ex-situ biofilm mediated approach for bioremediation of selected heavy metals in wastewater of textile industry

Kumar¹,

Shivani²,

Krishan³

et al. 2022

J App Biol biotech

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Industrialization plays a major role in strengthening the economy of any country. However, these industries directly or indirectly affect the environment. Industrial wastewater discharge has been reported with certain heavy metals such as chromium, lead, cobalt, and others which are a potential hazard to the water bodies and humans as well. Biofilm is an applied method in the fields of bioremediation for reliving this emerging problem and in the efficient removal of heavy metals from wastewater. Biofilms of Escherichia coli and petroleum soil isolated microorganisms (PSIM) were developed at the V bottom of 96 well microtiter plate. The contaminated water sample was collected from the textile industry, Solan, Himachal Pradesh, India. The biofilms were incubated with the industrial water tested for heavy metals assuming the microbes have the potential to assimilate the heavy metals up to 5 mg/mL of concentration. After the incubation for 1-2 weeks, the microorganisms were able to reduce the level of heavy metals present in the samples which was conveyed by biomass comparison of microorganisms in the successive intervals of time. 0.74 × 10 10 cells/mL and 0.77 × 10 10 cell/mL of E. coli and PSIM biofilms were able to tolerate the metal toxicity on incubation for 2 weeks at the highest concentration due to the functional group present extracellular polymeric substance which forms complexes with heavy metals. This leads to the fact that these biofilms have assimilated the heavy metals and are potent for the removal of heavy metals from industrial wastewater.

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“…The ATR analysis result showed that PG5/10-Rho has obvious EPS peak at 1,682 cm −1 (the amide I group C-O stretch) (Maurya et al, 2022), and the peak signal of PG5-Rho is more obvious than that of PG10-Rho (Figures 7A,B), which proves that Rho is more likely to produce organic secretions in PG5. In TG treatment, the peak position of EPS is not obvious, indicating that yeast has less secretion in TG, which may be attributed to the high F stress of TG (Figure 7B).…”

Section: Biomechanism Analysis Of Rho For Safe Utilization Of Pg and Tgmentioning

confidence: 92%

Red Yeast Improves the Potential Safe Utilization of Solid Waste (Phosphogypsum and Titanogypsum) Through Bioleaching

Chen

Yu-qi

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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Phosphogypsum (PG) and titanium gypsum (TG), as a by-product (solid waste) in phosphate fertilizer and titanium dioxide industry, are causing serious environmental hazards. The resource/harmless application of PG and TG is the development trend in the future. The biological function of red yeast (Rho: Rhodotorula mucilaginosa) can effectively reduce the concentration of pollutants in the environment and has the potential of biological flotation/purification of mineral solid waste. In this study, the bioremediation mechanism and safe utilization efficiency of Rho for different contents of PG and TG were explored by using its biological flotation function. The X-ray fluorescence spectrometry (XRF) results showed that F was the main toxic element in PG and TG, and Pb and Cd did not reach the detection limit. The processing capacity of Rho for PG (>10 g/ml) is higher than that of TG (<5 g/ml). After bioleaching by Rho, the proportion of F in PG and TG solid decreased by 61.45–63.79% and 49.45–59.19%, respectively. The results of three-dimensional fluorescence, extracellular polymeric substance (EPS) extraction, X-ray diffraction (XRD), and scanning electron microscopy (SEM) confirmed that Rho could accelerate the release of harmful elements (F) in PG and TG. SEM showed that Rho cells and secretions adhered and wrapped on PG/TG, causing PG/TG decomposition and fragmentation. In addition, the adsorption of EPS and the formation of Ca5(PO4)3F are two main ways for Rho to remove F. Furthermore, under the condition of high concentration bioleaching, Rho can accelerate the release and utilization of P in PG, which is not only for the re-precipitation of Ca5(PO4)3F but also conducive to the reproduction and utilization of microorganisms. Meanwhile, the purification/safe reuse of PG by Rho is easier than that of TG. Therefore, the toxicity of PG and TG bioleaching by Rho can be greatly reduced, suggesting the huge potential of Rho in soil improvement and remediation.

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Characterization of biofilm formation and reduction of hexavalent chromium by bacteria isolated from tannery sludge

Cited by 25 publications

References 48 publications

Stress Dependent Biofilm Formation and Bioactive Melanin Pigment Production by a Thermophilic Bacillus Species from Chilean Hot Spring

Stress Dependent Biofilm Formation and Bioactive Melanin Pigment Production by a Thermophilic Bacillus Species from Chilean Hot Spring

Ex-situ biofilm mediated approach for bioremediation of selected heavy metals in wastewater of textile industry

Red Yeast Improves the Potential Safe Utilization of Solid Waste (Phosphogypsum and Titanogypsum) Through Bioleaching

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