Characterization of biofilm formation in clinical urinary isolates of Staphylococcus aureus from five hospitals in Lagos State, Nigeria

Orjih, C.I.; Ajayi, A. A.; Alao, Felix Oluwasegun; Adeleye, Adeyemi Isaac; Smith, Stella I.

doi:10.4314/ajcem.v22i2.8

Cited by 5 publications

(1 citation statement)

References 10 publications

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“…At 37°C, plates were incubated aerobically for 24 to 48 hours. Black colonies with a dry crystalline appearance indicated positive outcomes, whereas non-biofilm producers remained pink in color 12 .…”

Section: Phenotyping Characterization Of Slime Producing Bacteria  B...mentioning

confidence: 99%

Isolation and identification of Biofilm-producing E. coli from drinking water.

Fatima

Mirani

Siddiqui

et al. 2022

Int. j. endorsing health sci. res.

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Background: The increasing rate of water-borne diseases in Karachi demands the characterization of associated pathogens. Nowadays, water-borne infections become more resistant to treatment due to biofilm formation and excessive use of antibiotics. Biofilm formation in water deteriorates the quality of water. This study identified and characterized biofilm-producing E. coli in drinking water. Methodology: Water samples were analyzed for heterotrophic plate count and total coliforms, fecal coliforms, and E. coli. The Kirby disk bar diffusion method was used to investigate antibiotic susceptibility in biofilm-producing E. coli. The Congo red and tube ring methods were used to identify biofilm producers. The effect of biofilm formation on the hydrophobicity of E. coli was performed by the BATH method. The soft agar method determines the colony spreading ability of biofilm and non-biofilm producers. Molecular characterization of virulence genes of E. coli was performed by a PCR. Scanning electron microscopy for biofilm construction was conducted. Results: The total 120 water samples were tested for heterotrophic plate count and total coliforms, fecal coliforms, and E. coli. 78% were unfit in this study, and 21.66% were fit. 38 E. coli strains were found in water samples. According to findings, the hydrophobicity of biofilm-producing isolates increased with the incubation period. Colony-forming unit drops one logarithm in the biofilm state compared to the planktonic stage. Biofilm producers were more resistant to antibiotics. The virulence genes, pet, lt, and stx2, were used for the molecular characterization. Conclusion: The presence of biofilm-producing E. coli in drinking water is alarming, and it indicates inappropriate treatment of the water supply system. To prevent rapid water-borne diseases, adequate actions are required to control drinking-water biofilm producers.

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Section: Phenotyping Characterization Of Slime Producing Bacteria  B...mentioning

confidence: 99%

Isolation and identification of Biofilm-producing E. coli from drinking water.

Fatima

Mirani

Siddiqui

et al. 2022

Int. j. endorsing health sci. res.

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Coexistence of the blaZ gene and selected virulence determinants in multidrug-resistant Staphylococcus aureus: insights from three Nigerian tertiary hospitals

Kilani,

Alabi,

Adeleke

2024

BMC Infect Dis

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Coexistence of the blaZ Gene and Selected Virulence Determinants in Multidrug-Resistant Staphylococcus aureus: Insights from Three Nigerian Tertiary Hospitals

Kilani,

Alabi,

Adeleke

2024

Preprint

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Background and Purpose Infections caused by β-lactamase-producing strains of Staphylococcus aureus have become increasingly difficult to treat due to the expression of multiple virulence factors. This has heightened concerns about managing S. aureus-related infections. This study was conducted to characterize the blaZ gene and selected virulence determinants in β-lactam resistant S. aureus from human sources in three Nigerian tertiary hospitals. Materials and Methods Three hundred and sixty samples were collected for the study. S. aureus was isolated and characterized following standard microbiological protocols and nuc gene amplification. Antibiotic susceptibility and minimum inhibitory concentration tests were performed using the disk diffusion method and E-tests, respectively. Biofilm formation and β-lactamase production were assessed using Congo red agar and nitrocefin kits, while the blaZ gene was examined using conventional PCR. Capsular polysaccharide genotyping, accessory gene regulator (agr) detection, Panton-valentine leucocidin (PVL), and PVL proteins were performed using PCR and Western blotting. Results S. aureus was recovered from 145 samples, 50 (34.5%) of these isolates exhibited multidrug resistance, with MICs ranging from 0.125 to 1.00 µg/mL, and showed significant resistance to aminoglycosides, fluoroquinolones, and β-lactams. Of these, 31 strains produced β-lactamases, 30 of which carried the blaZ gene in combination with cap8 (80%) or cap5 (20%). Biofilm formation and PVL gene were observed in 85% of the 20 randomly selected blaZ-positive multidrug-resistant (MDR) strains. The agr2 allele was predominant, found in 70% of the selected MDR strains. No significant difference in the occurrence of the blaZ gene was found among the three clinical sources (p ≤ α0.05). Conclusion The co-occurrence of the blaZ gene with PVL, capsular polysaccharide genes, and agr alleles is associated with biofilm formation, indicating a high risk of β-lactam-resistant S. aureus infections. Our findings highlight the need for continuous molecular surveillance to enhance infection management, treatment options, and patient outcomes in the study locality. A limitation of this study is the random selection of MDR isolates, which may affect the comprehensiveness of the analyses.

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Characterization of biofilm formation in clinical urinary isolates of Staphylococcus aureus from five hospitals in Lagos State, Nigeria

Cited by 5 publications

References 10 publications

Isolation and identification of Biofilm-producing E. coli from drinking water.

Isolation and identification of Biofilm-producing E. coli from drinking water.

Coexistence of the blaZ gene and selected virulence determinants in multidrug-resistant Staphylococcus aureus: insights from three Nigerian tertiary hospitals

Coexistence of the blaZ Gene and Selected Virulence Determinants in Multidrug-Resistant Staphylococcus aureus: Insights from Three Nigerian Tertiary Hospitals

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