Characterization of biosynthesized silver nanoparticles from Streptomyces aqueous extract and evaluation of surface-capping proteins involved in the process
“…Besides, the signals observed at 1461 and 1367 cm −1 are related to amide II groups ( Figure 5 B). These results were consistent with previous FTIR and Raman analysis of AgNPs ( Bakhtiari-Sardari et al., 2020 ; Paterlini et al., 2021 ; Zhang et al., 2016 ). Figure 4 Fourier transform infrared (FT-IR) spectra of silver nanoparticles (A) and the supernatant where nanoparticles were synthesized (B).…”
Section: Resultssupporting
confidence: 93%
“…In addition, these stabilizing or capping molecules were reported to improve the antimicrobial activity and avoid agglomeration of the nanoparticles. In a recent study, Paterlini et al. (2021) demonstrated that AgNPs synthetized by the strain Streptomyces sp.…”
Section: Resultsmentioning
confidence: 99%
“…M7 are associated to aminopeptidases, transaldolases, tyrosinases and NAD(P)-dependent oxidoreductases. These proteins of the surface of the AgNPs are disposed in different layers and play a key role in the stabilization ( Paterlini et al., 2021 ). In our study, EDS, FTIR and Raman spectra showed that organic molecules are associated to the surface of the AgNPs synthesized by A. tucumanensis .…”
Section: Resultsmentioning
confidence: 99%
“…Among the synthesis techniques used, the biological synthesis of silver nanoparticles emerged as an interesting environmental-friendly alternative ( Bansal et al., 2017a , 2017b ; Eid et al., 2020 ; Paterlini et al., 2021 ).…”
“…Besides, the signals observed at 1461 and 1367 cm −1 are related to amide II groups ( Figure 5 B). These results were consistent with previous FTIR and Raman analysis of AgNPs ( Bakhtiari-Sardari et al., 2020 ; Paterlini et al., 2021 ; Zhang et al., 2016 ). Figure 4 Fourier transform infrared (FT-IR) spectra of silver nanoparticles (A) and the supernatant where nanoparticles were synthesized (B).…”
Section: Resultssupporting
confidence: 93%
“…In addition, these stabilizing or capping molecules were reported to improve the antimicrobial activity and avoid agglomeration of the nanoparticles. In a recent study, Paterlini et al. (2021) demonstrated that AgNPs synthetized by the strain Streptomyces sp.…”
Section: Resultsmentioning
confidence: 99%
“…M7 are associated to aminopeptidases, transaldolases, tyrosinases and NAD(P)-dependent oxidoreductases. These proteins of the surface of the AgNPs are disposed in different layers and play a key role in the stabilization ( Paterlini et al., 2021 ). In our study, EDS, FTIR and Raman spectra showed that organic molecules are associated to the surface of the AgNPs synthesized by A. tucumanensis .…”
Section: Resultsmentioning
confidence: 99%
“…Among the synthesis techniques used, the biological synthesis of silver nanoparticles emerged as an interesting environmental-friendly alternative ( Bansal et al., 2017a , 2017b ; Eid et al., 2020 ; Paterlini et al., 2021 ).…”
“…Actinobacterial strains, such as Streptomyces, are also known to produce important natural bioactive compounds such as antibiotics, antitumors and antimetabolites [22][23][24]. There are some studies about the biological synthesis and characterization of AgNPs using a Streptomyces strain [25][26][27][28]. Antioxidative [28], antimicrobial, antibiofilm [29], in vitro mutagenic and antimutagenic [30] properties of the biosynthesized AgNPs using S. griseorubens AU2 were also reported by our team with previous reports.…”
Metallic nanoparticles are interesting areas of research due to their unique properties which can be advantageous for producing smart products. Silver nanoparticles (AgNPs) are remarkably used in pharmaceutical industry because of their strong biological activities. The aim of the present study was to investigate the cytotoxicity and wound healing capacity of the biologically prepared silver nanoparticles via green synthesis route. Cytotoxicity of the biogenic AgNPs was determined by MTT (3- [4, 5- dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium-bromide) assay against L929 fibroblast cell line. Wound healing properties of the AgNPs were evaluated using in vitro-scratch wound healing assay using 3T3 fibroblast cell line. Biosynthesized AgNPs inhibited the propagation of fibroblasts at a half maximal inhibitory concentration (IC50) of 23.507 μg/mL after 24 h incubation. In vitro wound healing assay also revealed that the biogenic AgNPs stimulated the 3T3 fibroblasts' cell proliferation. It can be suggested that biologically synthesized AgNPs can be used effectively for biomedical applications such as wound dressing materials.
The fast-growing urbanization and slow progress in the eld of waste management have led to the accumulation of large quantities of animal wastes. Present work focused on the synthesis of low-cost and eco-friendly chicken bile juice mediated silver nanoparticles (BJ-AgNP). Results reveals that bile juices have enough potentiality towards the synthesis of almost uniform sizes (average size < 50 nm) of BJ-AgNPs which remains stable for more than 6 months. Response surface methodology (RSM) successfully demonstrated the optimised condition of AgNP-BJ synthesis. Factor like concentration of salt and bile extract and temperature are signi cantly responsible for the synthesis of nanoparticle which was further characterized using UV-Vis, TEM, FESEM, XRD, FTIR, TGA, and EDS. The synthesised nanoparticle showed excellent bactericidal activity against both Gram positive and Gram negative bacteria with MIC and MBC of 40 and 50 µg/mL for Bacillus subtilis (MTCC-441) and 60 and 60 µg/mL for Eschecheria coli (MTCC-1687) respectively. The synthesized nanoparticle also acted as an antibio lm agent against Bacillus subtilis, with ~89% bio lm inhibition e cacy at 4 X MIC, having optimal bacterial concentration of 10 6 CFU/mL. Therefore, present ndings clearly demonstrated that an absolute animal waste could be a valuable ingredient in the eld of therapeutic nanoscience.
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