Characterization of Bone and Bone-Based Graft Materials Using FTIR Spectroscopy

ATR-FTIR Assessment of the Biochemical Composition and Micro Hardness of the Hard Tissues of Oral Cavity Submitted to Gamma Irradiation

“…These results suggest chemical composition is similar also in retromolar trigone region, where the difference is a low phosphate concentration. These results are confirmed by literature [42]. Irradiated retromolar trigone region spectra (Figure 20), show the same bands of irradiated samples in Figures 16-18.…”

“…The references (non-irradiated CM50REF and CM58REF) show the same spectral bands achieved in root dentin references that suggest mandibular body have the same compositional characteristics than root dentin. These results converge to those presented on the literature, where mandibular body presents 65% of inorganic material (poly hydroxy apatite and calcium phosphates), 25% of organic material (type I collagen fibers) and 10% of water [42]. Also, irradiated sample shows the same result observed to irradiated root dentin.…”

“…The infrared spectra of enamel region are showed in Figure 15 , related to asymmetric axial deformation of C-H bond from methylene group of hydrocarbon chain; at 1.731 cm -1 , related to axial deformation of C-O-O-bonds (peroxide group); at 1.465 cm -1 related to angular deformation from amide group (amide III) 46; at 1.165 cm -1 , corresponds to asymmetric axial deformation of C-O-C bonds (ether group); at 1.034 cm -1 and 873 cm -1 related to infrared absorption of PO43-(phosphate group) and at 715 cm -1 corresponding to out-of-plane angular deformation of hydroxyl group linked by hydrogen bonds. This analysis suggests sample is formed by proteins, an inorganic region and water that is according to dental enamel composition, presented in the literature [41][42][43][44] specifically 97% of poly hydroxy apatite, 1% of protein, being ameloblastine, enamelin, amelogenin and 2% of water [45][46][47]. Irradiated samples (E26_1708 and E18_1708) showed almost the same absorption bands, however, the band at 3.425 cm -1 disappeared, suggesting water removal and a band at 877 cm -1 is observed, suggesting some changes in poly hydroxy apatite inorganic region (Figure 16) shows irradiated and non-irradiated root dentine spectra.…”

“…In this case, this kind of sample has higher water concentration than enamel that leads water radiolysis when exposed to a gamma source. Mandibular body consists of 65% of inorganic material (polyhydroxy apatite and calcium phosphates), 25% of organic material (type I collagen fibers) and 10% of water, as well as dentin [42,44].…”

Implicações Ósseas na Sepse Sistêmica Aguda: Uma análise química e estrutural

“…None noise removal techniques were applied. [20]. The vibrational modes at ~1238 and ~1201 cm -1 are attributed to stretching vibrations of carboxyl group υCOO (amide III) and collagen, respectively [21].…”