Characterization of Borrelia burgdorferi sensu lato from Novosibirsk region (West Siberia, Russia) based on direct PCR

Abstract

Borrelia burgdorferi sensu lato infecting Ixodes persulcatus ticks near Novosibirsk, Russia were detected using PCR with primers specific to 5S and 23S rRNA genes. Two genospecies, B. afzelii and B. garinii, were identified by the PCR-based restriction fragment length polymorphism analysis with Tru9-I restriction endonuclease. Comparison of the corresponding nucleotide sequences revealed considerable diversity of the 5S-23S intergenic spacer structure among B. garinii.

Semi-Nested PCR Detection ofBartonella henselaeinIxodes persulcatusTicks from Western Siberia, Russia

“…Unfed adult Ixodes persulcatus ticks were collected from vegetation by flagging in suburban aspen-birch and pine forests near Novosibirsk (55°N, 83°E) in May 2002 and 2003 as previously described (Livanova et al, 2003). Nucleic acids were isolated by lysis of 100 individual ticks in 4M guanidine thiocyanate or using the IsoQuick Nucleic Acid Extraction Kit (ORCA Research, Bothell, WA) according to manufacturer's instructions, followed by phenol-chloroform extraction and isopropanol precipitation overnight.…”

PCR-detection and molecular-genetic analysis of tick-transmitted pathogens in patients of Novosibirsk region, Russia

“…garinii and Bo. afzelii) was detected in clinical samples as well as earlier in ticks and small mammals of Novosibirsk region (Korenberg et al, 2002;Livanova et al, 2003). It is necessary to note that B. garinii NT29 group prevailed in the investigated blood samples of patients with clinical diagnosis of early LB with erythema migrans (EM).…”

Multilocus sequence analysis of Borrelia burgdorferi s.l. in Russia

“…nov. The most significant difference between the phylogenies was the position of the genomic group ChY13p, whose specific PCR-RFLP sequence was more similar to those of B. afzelii (Livanova et al, 2003). However, analysis of the housekeeping genes (MLST) proved it to cluster with B. garinii 20047 (Fig.…”