Characterization of broad‐spectrum resistance to Soybean mosaic virus in soybean [Glycine max (L.) Merr.] cultivar ‘RN‐9’

Abstract

Soybean mosaic virus (SMV) can cause serious yield losses in soybean. Soybean cultivar ‘RN‐9’ is resistant to 15 of 21 SMV strains. To well‐characterize this invaluable broad‐spectrum SMV‐resistance, populations (F1, F2 and F2:3) derived from resistant (R) × susceptible (S) and R × R crosses were tested for SMV‐SC18 resistance. Genetic analysis revealed that SC18 resistance in ‘RN‐9’ plus two elite SMV‐resistant genotypes (‘Qihuang No.1’ and ‘Kefeng No.1’) are controlled by independently single dominant genes. Linkage analysis showed that the resistance of ‘RN‐9’ to SMV strains SC10, SC14, SC15 and SC18 is controlled by more than one gene(s). Moreover, Rsc10‐r and Rsc18‐r were both positioned between the two simple sequence repeats markers Satt286 and Satt277, while Rsc14‐r was fine‐mapped in 136.8‐kb genomic region containing sixteen genes, flanked by BARCSOYSSR_06_0786 and BARCSOYSSR_06_0790 at genetic distances of 3.79 and 4.14 cM, respectively. Allelic sequence comparison showed that Cytochrome P450‐encoding genes (Glyma.06g176000 and Glyma.06g176100) likely confer the resistance to SC14 in ‘RN‐9’. Our results would facilitate the breeding of broad‐spectrum and durable SMV resistance in soybeans.

Identification and Characterization of NPR1 and PR1 Homologs in Cymbidium orchids in Response to Multiple Hormones, Salinity and Viral Stresses

“…CymMV infection probably started with the ROS burst and accumulated SA, which in turn activated the CsNPR1-2 expression and CsPR1 genes (Figure 9). Traditionally, plant viruses can be easily mechanically inoculated into dicot hosts, such as soybean, tobacco and cucumber [56]. However, it is very difficult to mechanically inoculate the virus into the leathery leaves of orchids and some other monocot plants for plant-virus interaction study.…”

“…These orchid plants were obtained from the orchid breeding base of Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, China, and maintained in plastic pots (20 × 20 cm) in greenhouses with a favorable environment for growing as previously described [64]. Traditionally, plant viruses can be easily mechanically inoculated into dicot hosts, such as soybean, tobacco and cucumber [56]. However, it is very difficult to mechanically inoculate the virus into the leathery leaves of orchids and some other monocot plants for plant-virus interaction study.…”

Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of Soybean mosaic virus

“…However, Soybean mosaic virus (SMV; Potyvirus ), as one of the most prevalent viruses, can lead to serious seed quality deterioration and great yield losses (as high as 86%) of soybean under favorable conditions [ 2 , 3 , 4 , 5 , 6 , 7 ]. SMV, which is seed-borne and aphid-transmitted, usually causes soybean mosaic on leaves, local and systemic necrosis and plant dwarfing [ 8 , 9 , 10 ]. Highly diverse symptoms in soybean plants infected with SMV resulted in difficulties in the accurate recognition and early removal of the viral pathogen.…”

“…The double antibody sandwich–enzyme-linked immunosorbent assay (DAS-ELISA) is well known as a common immunological technique for virus detection and has now been widely used to detect many plant viruses, such as Potato virus S [ 30 ], Citrus yellow vein clearing virus (CYVCV) [ 31 ] and Zucchini yellow mosaic virus [ 32 ]. Commercial SMV-specific DAS-ELISA kits are available for laboratory use [ 9 , 23 , 33 ]. However, the coating antibody and the detecting conjugate of the imported DAS-ELISA kits were developed specific to the abroad SMV strains (G1-G7).…”

Characterization and allelism of soybean mosaic virus strain SC3 resistance in ‘Qihuang‐1’ derived soybean cultivars

“…Soybean is an important subsistence and economic crop. China is the fourth leading producer of soybean in the world and one of the vital diseases that affects flourishing soybean production in China is soybean mosaic disease caused by soybean mosaic virus (SMV) (Ren et al, 2018). SMV is a severe threat to major soybean‐producing regions.…”