Characterization of buffers for electrokinetic separations

Model for temperature profiles in large diameter electrochromatography columns

“…w T he m obile phase w as a 3.9-m M tris hydroxyx Ž . amino methane tris -47-mM glycine, pH 8.2 solution and was Ž selected due to its relatively low conductivity Basak et al, 1994;Rudge et al, 1993 . The buffer was cooled prior to entering the column using a series of cooling coils that were immersed in an ice bath.…”

“…For example, reducing the conductivity of the mobile and stationary phase re-Ž . duces joule heating Basak et al, 1994 . Research on electrochromatography column design and operation requires quantitative metrics by which column temperature as a function of position and time can be modeled and ultimately predicted. We developed a means of measuring the temperature inside the column without disrupting the Ž .…”

Mechanistic description and experimental studies of electrochromatography of proteins

“…Heat causes loss of zone resolution because of heat-induced convection, and possible denaturation of heat sensitive, biologically active compounds (Raj and Hunter, 1991). In order to obtain good separation of protein mixtures, it is also necessary to consider separation characteristics such as pH, ionic strength, type of stationary phase, buffer composition, and electrode design (Basak et al, 1994).…”

Protein adsorption‐dependent electro‐kinetic pore flow: Modeling of ion‐exchange electrochromatography with an oscillatory transverse electric field

“…On the other hand, the CP effect becomes weak when EDL is compressed by increasing ionic strength and EDL does not overlap in sufficiently large pores [23]. The ionic strength of the buffer in this work was 6.5 mmol/L (because the 3.9 mmol/L Tris-47 mmol/L Gly was reported to be 1.5 mmol/L [24]), so the EDL could be calculated to be 3.78 nm by the following equation…”