Characterization of Ca2+ transport in Euglena gracilis mitochondria

Uribe, Aída; Chávez, Edmundo; Jimenez, Moisés; Zazueta, Cecilia; Moreno‐Sánchez, Rafael

doi:10.1016/0005-2728(94)90141-4

Cited by 10 publications

(2 citation statements)

References 42 publications

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“…Isolated mitochondria of E. gracilis used NADH as an external substrate for oxygen consumption (Uribe et al 1994;Jasso-Chávez and Moreno-Sánchez 2003;Krnáčová et al 2015). This seems surprising at first sight, because NADH is generally thought not to be transported across the mitochondrial inner membrane, and the enzyme or enzymes responsible for the use of NADH as an exogenous substrate during oxygen consumption in E. gracilis mitochondria have not been identified (Krnáčová et al 2015).…”

Section: Aerobic Metabolic Pathwaymentioning

confidence: 93%

The Mitochondrion of Euglena gracilis

Zimorski

Rauch

Hellemond

et al. 2017

Advances in Experimental Medicine and Biology

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In the presence of oxygen, Euglena gracilis mitochondria function much like mammalian mitochondria. Under anaerobiosis, E. gracilis mitochondria perform a malonyl-CoA independent synthesis of fatty acids leading to accumulation of wax esters, which serve as the sink for electrons stemming from glycolytic ATP synthesis and pyruvate oxidation. Some components (enzymes and cofactors) of Euglena's anaerobic energy metabolism are found among the anaerobic mitochondria of invertebrates, others are found among hydrogenosomes, the H-producing anaerobic mitochondria of protists.

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Section: Aerobic Metabolic Pathwaymentioning

confidence: 93%

The Mitochondrion of Euglena gracilis

Zimorski

Rauch

Hellemond

et al. 2017

Advances in Experimental Medicine and Biology

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“…The cyanide-insensitive pathway is blocked by diphenylamine [37, 411, preferentially oxidizes lactate [4! ], is able to generate a small, uncoupler-sensitive membrane potential [22,411 and may drive an active Ca2+ transport [42]. Based on studies of antimycin and cyanide inhibition of respiration, a respiratory chain with two terminal oxidases and separate entries for oxidation of NADH, succinate and lactate has been proposed [4].…”

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confidence: 99%

The Content of Alternative Oxidase of Euglena Mitochondria is Increased by Growth in the Presence of Cyanide and is not Cytochrome o.

Devars¹,

Hernández²,

Covián³

et al. 1998

J Eukaryotic Microbiology

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A study of the effect of respiratory inhibitors on O2 uptake of Euglena gracilis mitochondria, isolated from cells grown in the presence of cyanide or with ethanol as carbon source, was undertaken. The contents of cytochrome c oxidase and alternative oxidase were also determined. Inhibition of respiration by antimycin and cyanide was only partial and it was dependent on the oxidizable substrate used. Succinate oxidation was the most sensitive to cyanide whereas lactate oxidation was the most resistant. Cell growth in the presence of cyanide or with ethanol as carbon source brought about an enhanced content of alternative oxidase without a concomitant increase in cytochrome aa3 content. However, a correlation between cyanide‐resistant respiration and alternative oxidase content was not found. Analysis of heme types in mitochondrial membranes revealed the absence of heme O. The data suggest the presence of an inducible alternative oxidase in Euglena mitochondria which has high resistance to cyanide and contains heme B. A close relationship between Euglena alternative oxidase and bacterial quinol oxidases containing B‐type heme is proposed.

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Lanthanide ions are agonists of transient gene expression in rice protoplasts and act in synergy with ABA to increase Em gene expression

Rock

Quatrano

1996

Plant Cell Reports

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Previous work has shown that in rice suspension cells, NaCl at 0.4 M can induce Em gene expression and act synergistically with ABA, possibly by potentiating the ABA response pathway through a rate-limiting intermediate (R.M. Bostock and R.S. Quatrano (1992) Plant Physiol., 98, 1356-1363). Since calcium is an intermediate in ABA regulation of stomatal closure, we tested the effect of calcium changes on ABA-inducible Em gene expression in transiently transformed rice protoplasts. We show that calcium is required for ABA-inducible Em-GUS expression and can act in synergy with ABA. The trivalent ions lanthanum, gadolinium, and aluminum, which are known to interact with calcium- and other signaling pathways, can act at sub-millimolar concentrations to increase GUS reporter gene expression driven by several promoters in transiently transformed rice protoplasts. This effect is not specific for the ABA-inducible Em promoter, but is synergistic with ABA. The lanthanum synergy with ABA does not require calcium. In rice suspension cells, lanthanum alone does not induce Em gene expression, in contrast to transiently transformed protoplasts, yet can act synergistically with ABA to effectively increase the sensitivity to ABA greater than tenfold. Trivalent ions may be a useful tool to study the regulation of gene expression. The possible effects of trivalent ions on ABA signal transduction and gene expression are discussed.

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Characterization of Ca2+ transport in Euglena gracilis mitochondria

Cited by 10 publications

References 42 publications

The Mitochondrion of Euglena gracilis

The Mitochondrion of Euglena gracilis

The Content of Alternative Oxidase of Euglena Mitochondria is Increased by Growth in the Presence of Cyanide and is not Cytochrome o.

Lanthanide ions are agonists of transient gene expression in rice protoplasts and act in synergy with ABA to increase Em gene expression

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