2022
DOI: 10.3389/fmicb.2022.883674
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Characterization of Calcium-Dependent Protein Kinase 2A, a Potential Drug Target Against Cryptosporidiosis

Abstract: Calcium-dependent protein kinases (CDPKs) are important in calcium influx, triggering several biological processes in Cryptosporidium spp. As they are not present in mammals, CDPKs are considered promising drug targets. Recent studies have characterized CpCDPK1, CpCDPK3, CpCDPK4, CpCDPK5, CpCDPK6, and CpCDPK9, but the role of CpCPK2A remains unclear. In this work, we expressed recombinant CpCDPK2A encoded by the cgd2_1060 gene in Escherichia coli and characterized the biologic functions of CpCDPK2A using qRT-P… Show more

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Cited by 3 publications
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“…A total of 48 compounds were selected from the ChemDiv database through the molecular docking of INS-16 as described previously [ 30 ] ( Table 1 ). The anti-cryptosporidial effect of these compounds at the concentration of 10 µM were assessed by using qRT-PCR in 44 h infection assays as described [ 31 ].…”
Section: Methodsmentioning
confidence: 99%
“…A total of 48 compounds were selected from the ChemDiv database through the molecular docking of INS-16 as described previously [ 30 ] ( Table 1 ). The anti-cryptosporidial effect of these compounds at the concentration of 10 µM were assessed by using qRT-PCR in 44 h infection assays as described [ 31 ].…”
Section: Methodsmentioning
confidence: 99%