Characterization of caprine herpesvirus 1 glycoprotein D gene and its translation product

Keuser, Véronique; Detry, Bruno; Thiry, Julien; Fays, Katalin de; Schynts, Frédéric; Pastoret, Paul-Pierre; Vanderplasschen, Alain; Thiry, Étienne

doi:10.1016/j.virusres.2005.07.009

Cited by 4 publications

(4 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Being CpHV-1 gD an immunodominant antigen and responsible for mounting a serum neutralizing humoral immune response in naturally and experimentally infected goats [24], [25], it was chosen as an ideally antigen to be expressed by BoHV-4 based vector. The full length CpHV-1 gD ORF was amplified by PCR from CpHV-1 infected cells DNA and sub cloned in-frame with a tag peptide, gD 106 [26] ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In fact BoHV-1 gD has been extensively used for vaccine development and its ORF has been genetically manipulated and expressed by prokaryotic and eukaryotic systems with variable results [21], [31], [32]. CpHV-1 gD ORF is 1224 nucleotides long and encodes for a protein of 407 amino acids with a predicted molecular mass of 44.3 kDa, a signal peptide comprising the first 17 amino acids, an hydrophobic region (amino acids 360–376) corresponding to its transmembrane domain near the carboxy terminus and two potential N -linked glycosylation sites (positions 41 and 102) [24]. However beyond CpHV-1 gD molecular characteristics no attempt concerning the use of CpHV-1 gD as an immunogen against CpHV-1 induced genital infection has been performed so far.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Clinical Protection of Goats against CpHV-1 Induced Genital Disease with a BoHV-4-Based Vector Expressing CpHV-1 gD

et al. 2013

View full text Add to dashboard Cite

Caprine herpesvirus type 1 (CpHV-1) is an alphaherpesvirus causing genital disease leading to abortion in adult pregnant goats and a systemic disease with high morbility and mortality in kids. Further, Caprine herpesvirus 1 infection represents a valuable large animal model for human herpesvirus induced genital disease, exploitable for pathogenic studies, new vaccines and antiviral molecules testing. Here, the bovine herpesvirus 4 (BoHV-4) based vector derived from an apathogenic isolate of BoHV-4 and expressing the immunodominant CpHV-1 glycoprotein D (BoHV-4-A-gDcpgD106ΔTK) was constructed and its ability to protect goats against CpHV-1 induced genital disease evaluated. The subcutaneous route of recombinant BoHV-4 administration was first tested in vivo/ex vivo by in vivo image analysis and in vitro by goat skin primary cultures preparation and transduction. Next, an exploratory immunization and safety study in goats was performed with two recombinant BoHV4, BoHV-4-A-gDcpgD106ΔTK or BoHV-4-CMV-IgK-gE2gD-TM. In both cases no clinical signs were evident but a good titer of serum neutralizing antibodies was produced in all inoculated animals. When a challenge experiment was performed in a new group of animals using a highly pathogenic dose of CpHV-1, all the vaccinated goats with BoHV-4-A-gDcpgD106ΔTK were protected toward CpHV-1 induced genital disease respect to the unvaccinated control which showed typical vaginal lesions with a high grade of clinical score as well as a long lasting viral shedding. In summary, the data acquired in the present study validate BoHV-4-based vector as a safe and effective viral vector for goat vaccination against CpHV-1 induced genital disease and pave the way for further applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Clinical Protection of Goats against CpHV-1 Induced Genital Disease with a BoHV-4-Based Vector Expressing CpHV-1 gD

et al. 2013

View full text Add to dashboard Cite

show abstract

“…CpHV-1 gD contains complex N-linked oligosaccharides, but the presence of O-glycosylation has not been demonstrated. In contrast with BoHV-1 gD, CpHV-1 gD is seemingly expressed as a late protein [64].…”

Section: Viral Glycoproteinsmentioning

confidence: 98%

“…Based on the complete gB gene sequence, which is the most conserved between herpesviruses [48], the percentages of nucleotide sequence identity with BoHV-1 gB are 78.5% for CpHV-1, 84% for CvHV-2, 85.8% for CvHV-1, 87.4% for BoHV-5 and 87.7% for BuHV-1 [107]. The gC 2 and gD gene sequences are fully known for each ruminant alphaherpesviruses, except for BuHV-1 [26,29,64,112]. On this basis, the percentages of amino acid sequence identity between BoHV-1 and ElkHV-1 are 71.7% (gC) or 72.9% (gD) [29].…”

Section: Genomic Comparison Between Ruminant Alphaherpesvirusesmentioning

confidence: 99%

Ruminant alphaherpesviruses related to bovine herpesvirus 1

et al. 2006

Self Cite

View full text Add to dashboard Cite

-Herpesviruses have mainly co-evolved with their hosts for millions of years. Consequently, different related host species may have been infected by various genetically related herpesviruses. Illustrating this concept, several ruminant alphaherpesviruses have been shown to form a cluster of viruses closely related to bovine herpesvirus 1 (BoHV-1): namely bovine herpesvirus 5, bubaline herpesvirus 1, caprine herpesvirus 1, cervid herpesviruses 1 and 2 and elk herpesvirus 1. These viruses share common antigenic properties and the serological relationships between them can be considered as a threat to BoHV-1 eradication programmes. BoHV-1 is a herpesvirus responsible for infectious bovine rhinotracheitis, which is a disease of major economic concern. In this article, the genetic properties of these ruminant alphaherpesviruses are reviewed on a comparative basis and the issue of interspecific recombination is assessed. The pathogenesis of these infections is described with emphasis on the host range and crossing of the host species barrier. Indeed, the non bovine ruminant species susceptible to these ruminant alphaherpesviruses may be potential BoHV-1 reservoirs. The differential diagnosis of these related infections is also discussed. In addition, available epidemiological data are used to assess the potential of cross-infection in ruminant populations. A better knowledge of these ruminant alphaherpesvirus infections is essential to successfully control infectious bovine rhinotracheitis.

show abstract

Deletion of the thymidine kinase gene attenuates Caprine alphaherpesvirus 1 in goats

Noll

Joshi

Nascimento

et al. 2019

Veterinary Microbiology

View full text Add to dashboard Cite

Characterization of caprine herpesvirus 1 glycoprotein D gene and its translation product

Cited by 4 publications

References 57 publications

Clinical Protection of Goats against CpHV-1 Induced Genital Disease with a BoHV-4-Based Vector Expressing CpHV-1 gD

Clinical Protection of Goats against CpHV-1 Induced Genital Disease with a BoHV-4-Based Vector Expressing CpHV-1 gD

Ruminant alphaherpesviruses related to bovine herpesvirus 1

Deletion of the thymidine kinase gene attenuates Caprine alphaherpesvirus 1 in goats

Contact Info

Product

Resources

About