Characterization of carbapenem-non-susceptible Escherichia coli isolates from a university hospital in Taiwan

Liu, Yi Fang; Yan, Jing Jou; Ko, Wen Chien; Tsai, Shu Huei; Wu, Jiunn Jong

doi:10.1093/jac/dkn049

Cited by 37 publications

(29 citation statements)

References 11 publications

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“…Unlike OmpA, the variation in OmpC may be much higher than we thought. Our previous data showed a higher mutation rate of the ompC gene (truncated forms or insertion) in multidrug-resistant E. coli (23,41). We also found that the OmpC mutant can be detected from natural selection, and these mutants not only had similar carbapenems and cefepime resistance, but also had similar serum bactericidal activities ( Fig.…”

Section: Discussionsupporting

confidence: 65%

“…E. coli 2837-2/05, producing the CMY-2 AmpC enzyme and TEM-1 narrow-spectrum ␤-lactamase, was isolated from an intra-abdominal drainage culture (23). This strain expressed only OmpA and OmpC, without OmpF, and was used for mutant construction and protein expression.…”

Section: Methodsmentioning

confidence: 99%

“…OMPs were prepared as described previously and separated by sodium dodecyl sulfate-10% polyacrylamide gel electrophoresis (SDS-PAGE) with 4 M urea (23). The gels were stained with 0.1% Coomassie blue.…”

Section: Methodsmentioning

confidence: 99%

“…The expression of OmpC and OmpF is regulated by osmolarity (14,42), and loss of OmpC or OmpF is related to antibiotic resistance, particularly when combined with production of extended-spectrum ␤-lactamases and AmpC (11,24,34). Previously, we have demonstrated that resistance to carbapenems in E. coli is related to the loss of OmpC expression (23). The loss of OmpC expression may be due to selection under antibiotic pressure.…”

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confidence: 98%

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Loss of Outer Membrane Protein C in Escherichia coli Contributes to Both Antibiotic Resistance and Escaping Antibody-Dependent Bactericidal Activity

et al. 2012

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ABSTRACTOuter membrane proteins (OMPs) serve as the permeability channels for nutrients, toxins, and antibiotics. InEscherichia coli, OmpA has been shown to be involved in bacterial virulence, and OmpC is related to multidrug resistance. However, it is unclear whether OmpC also has a role in the virulence ofE. coli. The aims of this study were to characterize the role of OmpC in antimicrobial resistance and bacterial virulence inE. coli. TheompCdeletion mutant showed significantly decreased susceptibility to carbapenems and cefepime. To investigate the survival ofE. coliexposed to the innate immune system, a human blood bactericidal assay showed that theompCmutant increased survival in blood and serum but not in complement-inactivated serum. These effects were also demonstrated in the natural selection of OmpC mutants. Also, C1q interacted withE. colithrough a complex of antibodies bound to OmpC as a major target. Bacterial survival was increased in the wild-type strain in a dose-dependent manner by adding free recombinant OmpC protein or anti-C1q antibody to human serum. These results demonstrated that the interaction of OmpC-specific antibody and C1q was the key step in initiating the antibody-dependent classical pathway for the clearance of OmpC-expressingE. coli. Anti-OmpC antibody was detected in human sera, indicating that OmpC is an immunogen. These data indicate that the loss of OmpC inE. coliis resistant to not only antibiotics, but also the serum bactericidal effect, which is mediated from the C1q and anti-OmpC antibody-dependent classical pathway.

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Section: Discussionsupporting

confidence: 65%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 98%

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Loss of Outer Membrane Protein C in Escherichia coli Contributes to Both Antibiotic Resistance and Escaping Antibody-Dependent Bactericidal Activity

et al. 2012

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“…Soon afterward, IMP-1 was prevalent in Serratia marcescens and spread into several members of the family Enterobacteriaceae in Japan (Walsh et al, 2005;Queenan and Bush, 2007). There has been an increase in the detection and spread of the acquired MBLs in Enterobacteriaceae from Australia and Taiwan region of China, and IMP-4 and IMP-8 were the predominant MBLs (Walsh et al, 2005;Queenan and Bush, 2007;Peleg et al, 2005;Herbert et al, 2007;Wu et al, 2007;2008;Liu et al, 2008). MBLs in Enterobacteriaceae have occurred sporadically in other countries.…”

Section: Introductionmentioning

confidence: 99%

Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate

Chen

Zhou

Cai

et al. 2009

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To investigate the mechanism of carbapenem resistance and the occurrence of plasmid-mediated quinolone resistance determinants qnr and aac(6′)-Ib-cr in a clinical isolate of Enterobacter cloacae. Methods: An ertapenem-resistant E. cloacae ZY106, which was isolated from liquor puris of a female gastric cancer patient in a Chinese hospital, was investigated. Antibiotic susceptibilities were determined by agar dilution method. Conjugation experiments, isoelectric focusing, polymerase chain reaction (PCR), and DNA sequence analyses of plasmid-mediated carbapenemases and quinolone resistance determinants were preformed to confirm the genotype. Outer membrane proteins (OMPs) were examined by urea-sodium dodecyl sulfatepolyacrylamide gel electrophoresis (Urea-SDS-PAGE). Results: Minimum inhibitory concentrations (MICs) of imipenem, meropenem, and ertapenem for ZY106 were 2, 4, and 16 μg/ml, respectively. Conjugation studies with Escherichia coli resulted in the transfer of significantly reduced carbapenem susceptibility. ZY106 produced IMP-1 metallo-β-lactamase and CTX-M-3 extended-spectrum β-lactamase, and E. coli transconjugant produced IMP-1. Plasmid-mediated quinolone resistance determinant qnrS1 was detected in ZY106. Transfer of the qnrS1-encoding-plasmid into E. coli by conjugation resulted in intermediate resistance to ciprofloxacin in E. coli transconjugant. Urea-SDS-PAGE analysis of OMPs showed that ZY106 lacked an OMP of approximately 38 kDa. Conclusion: It is the first IMP-1-producing Enterobacteriaceae in China and the first report of a clinical isolate that harbors both blaIMP and qnrS genes as well. The blaIMP-1, blaCTX-M-3, and qnrS1 are encoded at three different plasmids. IMP-1 combined with the loss of an OMP possibly resulted in ertapenem resistance and reduced imipenem and meropenem susceptibility in E. cloacae.

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Prevalence and characteristics of ertapenem-nonsusceptible Escherichia coli in a Taiwanese university hospital, 1999 to 2007

Yan

Lee

et al. 2010

Eur J Clin Microbiol Infect Dis

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The present study was conducted to investigate the prevalence and characteristics of ertapenem-nonsusceptible (ETP-NS) Escherichia coli in a Taiwanese university. A total of 9,722 isolates collected in 1999, 2003, 2005, and 2007 were examined. Overall, 1.0% of all isolates from 66 patients were interpreted as ETP-NS on the basis of the disk diffusion test result. Most of these isolates were clonally unrelated and showed low-level ertapenem resistance, the production of CMY-2 cephalosporinase (86.4%), and decreased expression of the OmpF (97.0%) and/or OmpC (56.1%) porins. No carbapenemase was detected. The decreased porin expression was associated with disruptions of ompF or ompC in only about one-third of the ETP-NS isolates. During the study period, the prevalence of ETP-NS strains increased from 0.1 to 1.7%, accompanying an increase (0.8 to 17.6%) in the prevalence of CMY-2 producers. Coexistent or pre-existing clonally related ertapenem-susceptible (ETP-S) E. coli isolates were identified in 47.0% of all case patients, and almost all of the ETP-S isolates had the same β-lactamases as the ETP-NS isolates. Our study results suggest the restricted use of extended-spectrum cephalosporins to hinder the emergence and prevalence of carbapenem resistance in E. coli, which may arise by the accumulation of multiple resistance determinants.

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Characterization of carbapenem-non-susceptible Escherichia coli isolates from a university hospital in Taiwan

Abstract: Our study suggests that the decreased susceptibility to carbapenems in E. coli in the hospital might arise by the stepwise accumulations of multiple drug-resistance determinants in different clones.

Cited by 37 publications

References 11 publications

Loss of Outer Membrane Protein C in Escherichia coli Contributes to Both Antibiotic Resistance and Escaping Antibody-Dependent Bactericidal Activity

Loss of Outer Membrane Protein C in Escherichia coli Contributes to Both Antibiotic Resistance and Escaping Antibody-Dependent Bactericidal Activity

Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate

Prevalence and characteristics of ertapenem-nonsusceptible Escherichia coli in a Taiwanese university hospital, 1999 to 2007

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