Characterization of carbapenem resistant acinetobacter baumannii isolated from intensive care units in two teaching hospitals from Algeria and Tunisia

Amiri, Sabrina; Hammami, Samia; Amoura, Kamel; Dekhil, Mazouz; Boubaker, Ilhem Boutiba-Ben

doi:10.11604/pamj.2017.28.19.9713

Cited by 2 publications

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“…Of these, OXA‐51 is intrinsic and the acquisition of other CHDL genes occurs through plasmids, integrons, or transposons. In addition to β‐lactamases, increased activity of multidrug efflux pumps, decreased permeability of cell membranes, or modified penicillin‐binding proteins can give rise to the emergence of CRAB strains (Franolić‐Kukina et al ; Amiri et al ; Ning et al ). Meanwhile, insertion sequences (ISs) are mobile genetic elements that can serve as a strong promoter for resistance genes.…”

Section: Introductionmentioning

confidence: 99%

Clonal relatedness and resistance characteristics of OXA‐24 and ‐58 producing carbapenem‐resistantAcinetobacter baumanniiisolates in Tehran, Iran

et al. 2019

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Aims: The present study was conducted to investigate the mechanism of carbapenem resistance and the molecular epidemiology of carbapenem-resistant Acinetobacter baumannii (CRAB) isolates collected from two nearby hospitals in Tehran, Iran. Methods and Results: A total of 180 CRAB isolates were studied. Antimicrobial susceptibility testing was performed using disk diffusion and Epsilometer tests. The detection of OXA-23, -24 and -58 was implemented for all isolates using polymerase chain reaction. Subsequently, isolates harbouring OXA-24 and -58 were investigated for the presence of resistance determinants of Ambler class A, metallo-b-lactamases (MBLs), and carbapenem-hydrolysing class D b-lactamases, ISAba1, and the genetic relatedness between them was analysed using pulsed-field gel electrophoresis (PFGE). All isolates were found to be resistant to imipenem with a MIC of ≥8 µg ml À1 and were susceptible to colistin with a MIC of ≤1Á5 µg ml À1 . Sixty percent of the isolates had OXA-23. OXA-24 and -58 were detected in 31 of 180 CRAB isolates. These chosen isolates were devoid of MBLs and bla SHV , bla CTX-M , bla VEB ESBL genes. The PER determinant was detected in 38% of isolates as the most common extended spectrum b-lactamases (ESBLs). Of these isolates, 51Á6% had OXA-23, and ISAba1 was found to be upstream of OXA-23 and OXA-51 in 16 and 8 isolates, respectively. The band patterns produced by PFGE showed nine clonal pulsotypes distributed between the two hospitals. Conclusion: The findings showed that the refractory CRAB isolates were transmitted intra-and inter-hospital, particularly in the ICU due to shortcomings in infection control surveillance. Significance and Impact of the Study: Carbapenem resistance is a substantial threat in the treatment of infections caused by A. baumannii due to limitations in the therapeutic options.

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Section: Introductionmentioning

confidence: 99%

Clonal relatedness and resistance characteristics of OXA‐24 and ‐58 producing carbapenem‐resistantAcinetobacter baumanniiisolates in Tehran, Iran

et al. 2019

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“…In Algeria, the first descriptions of NDM-1 in Algerian patient were reported by Boulanger and Bogaerts in 2011 [33,34]. After, this MBL was increasingly reported by other authors in Western: Oran [18], Eastern: Annaba [35,36] and Central Algeria: Tizi-Ouzou (Azzam et al34th RICAI 2014, France), Algiers, Bejaia and Setif [19,[37][38][39].…”

Section: Molecular Detection Of Resistance Genesmentioning

confidence: 95%

Phenotypic and genotypic characterization of multidrug-resistant Acinetobacter baumannii isolated in Algerian hospitals

Benamrouche

Lafer

Benmahdi

et al. 2020

J Infect Dev Ctries

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Introduction: The aim of this study was to investigate the drug-resistance and the molecular characterization of carbapenemases, ESBL, and aminoglycoside-modifying enzymes among Acinetobacter baumannii clinical isolates in Algerian hospitals. Methodology: A total of 92 A. baumannii isolates were collected between 2012 and 2016. Antimicrobial susceptibility testings were performed for β-lactams, aminoglycosides, fluoroquinolones, trimethoprim-sulfamethoxazole, rifampicin and colistin. The phenotypic characterization of β-lactamases was investigated. For 30 randomly targeted strains, the carriage of the carbapenemases, ESBL and aminoglycoside-modifying enzymes -encoding genes was determined by PCR. Sequencing was carried out for carbapenemases and ESBL genes. Results: Most of the 92 isolates studied were recovered from hospitalized patients (93.5%) and were mainly from intensive care units (51.1%) and orthopedics (19.6%). The strains were collected primarily from low respiratory tract (33.7%), wounds (23.9%) and urine (16.3%). Multidrug-resistant A. baumannii strains were prevalent (96.7%). High rates of resistance were observed for almost all antibiotics tested (>70%) excluding rifampicin (7.6%) and colistin (5.4%). For the five colistin-resistant strains, MICs ranged between 4 and 128 µg/mL. Positive MBL (83.7%) and ESBL (23.9%) strains were identified. Regarding β-lactams, the blaNDM and both blaSHV and blaCTX-M1 genes were detected in five and two strains respectively. Sequencing of the genes revealed the presence of blaNDM-1, blaCTX-M-15, and blaSHV-33. For aminoglycosides, aac(6’)-Ib, ant(2’’)-I and aph(3’)-VI genes were detected in three, seven and six strains respectively. Conclusions: Here, we report the first co-occurrence of extended-spectrum β-lactamases SHV-33 and CTX-M-15, the carbapenemase NDM-1 and the emergence of colistin-resistant A. baumannii in Algerian hospitals.

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Characterization of carbapenem resistant acinetobacter baumannii isolated from intensive care units in two teaching hospitals from Algeria and Tunisia

Cited by 2 publications

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Clonal relatedness and resistance characteristics of OXA‐24 and ‐58 producing carbapenem‐resistantAcinetobacter baumanniiisolates in Tehran, Iran

Clonal relatedness and resistance characteristics of OXA‐24 and ‐58 producing carbapenem‐resistantAcinetobacter baumanniiisolates in Tehran, Iran

Phenotypic and genotypic characterization of multidrug-resistant Acinetobacter baumannii isolated in Algerian hospitals

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