Characterization of carbapenemases, ESBLs, and plasmid-mediated quinolone determinants in carbapenem-insensitive Escherichia coli and Klebsiella pneumoniae in Riyadh hospitals

Al-Agamy, Mohamed H.; Aljallal, Abduelah; Radwan, Hesham H.; Shibl, Atef M.

doi:10.1016/j.jiph.2017.03.010

Cited by 61 publications

(63 citation statements)

References 20 publications

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“…Previously, the rates of bla SHV containing Enterobacterales with a focus on uropathogenic isolates were reported 12.2% to 54.8% by Iranian authors. [24][25][26][27][28][29] Meanwhile, several authors have shown the global spared of SHV type β-lactamases in uro- pathogenic Enterobacterales from China, 30 India, 31 Saudi Arabia, 32 Turkey, 33 Ukraine, 34 and Morocco. 35 In our results, 4.1% of uropathogenic Enterobacterales contained bla NDM-1, which to the best of our knowledge, is the first prevalence of this gene in Guilan province (North of Iran).…”

Section: Discussionmentioning

confidence: 99%

“…Previously, Alizadeh et al, in a study conducted in the southwest of Iran (Kerman), showed that 17.2% of uropathogenic isolates were OXA-1 producers. 20 Reviewing global reports such as Brazil (60%), 38 Portugal (55%), 39 Saudi Arabia (54.8%, and 48.3%), 32,40 and Bangladesh (47.5%), 41 indicate that the occurrence of OXA-1 β-lactamases can be varied based on type and source of isolates and the prevalence of ESBLs in each region.…”

Section: Discussionmentioning

confidence: 99%

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The Phylogenetic Relatedness of blaNDM-1 Harboring Extended-Spectrum β-Lactamase Producing Uropathogenic Escherichia coli and Klebsiella pneumoniae in the North of Iran

Jamali¹,

Tavakoly²,

Mojtahedi³

et al. 2020

IDR

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Background: Escherichia coli and Klebsiella pneumoniae as an important part of Enterobacterales family are important causes of both community-and hospital-acquired infections. The present study aimed to investigate the prevalence of antibiotics resistance and molecular characteristics of uropathogenic isolates of E. coli and K. pneumoniae in Iranian patients. Methods: This cross-sectional study performed on 223 Escherichia coli and 68 Klebsiella pneumoniae isolates obtained from hospitalized patients in the north of Iran. The isolates were identified by standard microbiologic tests and confirmed by API 20E strip. Disk diffusion method was applied to determine antibiotic susceptibility pattern. The presence of β-lactamases encoding genes was evaluated by PCR method. Analysis of the mutations and homology among sequences was done by the CLC sequence viewer (Qiagen, Denmark), and phylogenetic trees were constructed by the neighbor-joining method (Bootstrap: 1000 times). Results: The overall rates of extended-spectrum β-lactamases (ESBLs)-producing E. coli and K. pneumoniae isolates were 37.7% and 32.4%, respectively. The overall presence of bla SHV , bla NDM-1 , and bla OXA-1 genes was detected in 16 (5.5%), 12 (4.1%), and 48 (16.4%) of isolates, respectively. The neighbor-joining analysis for E. coli KU985246.1 strain showed that the most related bla NDM-1 sequences were from China, Singapore, UK, Thailand, and Bangladesh. While K. pneumoniae KU985245.1 strains were mostly related to bla NDM-1 sequences form Myanmar, and China. Conclusion: In summary, the remarkable rate of ESBL-producing uropathogenic Enterobacterales along with the first prevalence of NDM-1 β-lactamases can be a serious concern in our region.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Phylogenetic Relatedness of blaNDM-1 Harboring Extended-Spectrum β-Lactamase Producing Uropathogenic Escherichia coli and Klebsiella pneumoniae in the North of Iran

Jamali¹,

Tavakoly²,

Mojtahedi³

et al. 2020

IDR

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“…In a different way, bla IMP was detected in single isolate only. Reports of OXA-48 carbapenemases have been published worldwide, including Middle East and North Africa countries, for example, Saudi Arabia, Sultanate of Oman, Qatar, United Arab Emirates, Turkey, Egypt, Tunisia, Morocco, Libya and Lebanon [6,7,10,11,[20][21][22]. The results presented here provide the first insight into the coproduction of the OXA-48 like carbapenemase encoding gene and NDM-type representing MBL production in K. pneumoniae as well as the association of OXA-48 like and IMP-1 like among hospitalized cancer patients in Cairo, Egypt.…”

Section: Discussionmentioning

confidence: 99%

“…In the Middle East Northern African countries, such as Morocco, Algeria, Tunisia, Libya, Egypt, or Saudi Arabia, OXA-48-producing K. pneumoniae have been predominantly described [5,6]. On the other hand, in some regions of the Arabian Peninsula, NDM-1 was the experienced carbapenemases to the greatest extent followed by OXA-48-like carbapenemases [7].…”

Section: Introductionmentioning

confidence: 99%

Dissemination of ST101 blaOXA-48 producing Klebsiella pneumoniae at tertiary care setting

El-Mahallawy

Zafer

Al-Agamy

et al. 2018

J Infect Dev Ctries

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Introduction: The worldwide dissemination of the acquired carbapenemases in Gram-negative bacteria is a strongly expressed demand for the emergence of post antibiotic era. The aim of this study was to test the production of carbapenemase by Klebsiella pneumoniae strains isolated from hospitalized cancer patients and to investigate the genetic relationship of carbapenemase producing carbapenem resistant K. pneumoniae using multilocus sequence typing (MLST). Methodology: Antibiotic susceptibility testing and phenotypic testing for extended spectrum b-lactamases (ESBL) and carbapenemases production were performed. PCR amplification of ESBL and carbapenemase genes was performed. MLST was done to detect the genetic relatedness of the isolates. Results: Our data showed all strains were sensitive to colistin. Carba NP test was positive in thirty-one carbapenem resistant K. pneumoniae isolates and 26 out of 34 K. pneumoniae isolates were metallo-beta-lactamases (MBL) positive. All carbapenemase-positive isolates were ESBL CTX-M-1-like positive. blaOXA-48 gene was detected in 25 isolates (80.65%) and 21 isolates (67.75%) produced blaNDM-1 like enzyme. VIM and KPC genes were not identified in this study. Association of blaOXA-48 like and blaNDM-1 like was found in 15 (48.39%) isolates, while the coproduction of OXA-48-like and IMP-1 was revealed in only one K. pneumoniae isolate. MLST revealed ten distinct sequence types (STs). Conclusion: Here we have documented the coexistence of NDM-type and OXA-48-like, and the coproduction of OXA-48-like and IMP in carbapenem resistant K. pneumoniae in patients with cancer. The dominant clone of the OXA-48-like-producing K. pneumoniae isolates from Egypt was ST101 epidemic clone belonging to clonal complex 101, an association that has been reported worldwide. The second most frequent ST was ST383.ST11 was assigned to OXA-48-producing K. pneumoniae.

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“…The colistin was the most promising antibiotic with the 84% sensitivity [40]. 60% of the isolates were resistant to imipenem (IMI), and ertapenem (ERT) in a study reported from Riyadh, Saudi Arabia [41].…”

Section: Leseva Et Al Reported a Positive Correlation Between The Mmentioning

confidence: 99%

Risk factors analysis: screening of extended-spectrum-β-lactamase producing Gram-negative isolates of burn infections from tertiary care hospital Lahore, Pakistan

Haider

Ain

Hussain

et al. 2019

Preprint

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Background Burnt patients are highly susceptible to invasion of multidrug resistant strains after the skin damage. The main objective of this study was to estimate the frequency of ESBLs producing Gram-negative in post-burn infections and its correlation with different risk factors (age, gender, socio-economic status, burns etiology etc.) Methods The descriptive case-series study was conducted at Jinnah Hospital Lahore’s Burn and Reconstructive Surgery Centre (JB & RSC) and the Department of Microbiology and Molecular Genetics (MMG), University of the Punjab, Lahore. The clinical specimens of wound swabs, biopsy, and blood were collected from 300 patients during 12th August, 2017 to 12th August 2018. The cephalosporins resistant Gram-negative isolates were further analyzed. The clinical history of burnt patients was recorded which included the age, gender, socio-economic status, residence, occupation, hospital stay, wards, burn degree, total burnt surface area (TBSA%), etiology of burn and other factors. CLSI guidelines 2017 were followed for the antimicrobial susceptibility testing (AST) and ESBLs screening. Results Pseudomonas spp. were the most-frequently isolated 49.33% (n=74) followed by 22.67% (n=34) Klebsiella spp., and 20.00% (n=30) Acinetobacter spp., strains. Pseudomonas spp., were the most frequently isolated from burnt specimens 70 (46.67%) having a significant correlation (x2=24.11, p < 0.001). Community acquired infections were observed in 50.70% (n=76) and nosocomial infections in 49.30% (n=74) patients. Burnt people having the age of ≤ 29 years were found to be significantly associated with the MDR infections (x2=24.96, p = 0.003). Low socio-economic status, longer hospitalization and all other risk factors had non-significant correlation (p > 0.05). A large fraction of the isolates 86.00% (n=129) were not confirmed as ESBLs producers by phenotypic screening. Conclusion It has been concluded that Gram-negative MDR strains are rapidly causing infections in burnt patients and need to be properly alleviated. The high frequency of multidrug resistant Pseudomonas spp., was associated with the burn infections. The patients belonging to young age were significantly found to be infected by MDR strain after burns.

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Characterization of carbapenemases, ESBLs, and plasmid-mediated quinolone determinants in carbapenem-insensitive Escherichia coli and Klebsiella pneumoniae in Riyadh hospitals

Cited by 61 publications

References 20 publications

<p>The Phylogenetic Relatedness of <em>bla</em>N<sub>DM-1</sub> Harboring Extended-Spectrum β-Lactamase Producing Uropathogenic <em>Escherichia coli</em> and <em>Klebsiella pneumoniae</em> in the North of Iran</p>

<p>The Phylogenetic Relatedness of <em>bla</em>N<sub>DM-1</sub> Harboring Extended-Spectrum β-Lactamase Producing Uropathogenic <em>Escherichia coli</em> and <em>Klebsiella pneumoniae</em> in the North of Iran</p>

Dissemination of ST101 blaOXA-48 producing Klebsiella pneumoniae at tertiary care setting

Risk factors analysis: screening of extended-spectrum-β-lactamase producing Gram-negative isolates of burn infections from tertiary care hospital Lahore, Pakistan

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