Characterization of carbohydrate metabolism and demonstration of glycosomes in a Phytomonas sp. isolated from Euphorbia characias

Sánchez‐Moreno, Manuel; Lásztity, Demeter; Coppens, Isabelle; Opperdoes, Frederik

doi:10.1016/0166-6851(92)90111-v

Cited by 97 publications

(75 citation statements)

References 27 publications

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“…Indeed, AK1 can physically interact with phosphofructokinase and participate in the formation of a larger glycolytic enzyme complex or cluster (30,31,48). Whether this complex only serves to provide localized glycolytic ADP:ATP phosphotransfer capacity, presumably important for sustaining actomyosin sliding and force production, or also has any other structuralorganizational function is currently unknown.…”

Section: Discussionmentioning

confidence: 99%

Adenylate Kinase 1 Deficiency Induces Molecular and Structural Adaptations to Support Muscle Energy Metabolism

Janssen¹,

Groof²,

Wijers³

et al. 2003

Journal of Biological Chemistry

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Genetic ablation of adenylate kinase 1 (AK1), a member of the AK family of phosphotransfer enzymes, disturbs muscle energetic economy and decreases tolerance to metabolic stress, despite rearrangements in alternative high energy phosphoryl transfer pathways. To define the mechanisms of this adaptive response, soleus and gastrocnemius muscles from AK1 ؊/؊ mice were characterized by cDNA array profiling, Western blot and ultrastructural analysis. We demonstrate that AK1 deficiency induces fiber-type specific variation in groups of transcripts involved in glycolysis and mitochondrial metabolism and in gene products defining structural and myogenic events. This was associated with increased phosphotransfer capacities of the glycolytic enzymes pyruvate kinase and 3-phosphoglycerate kinase. Moreover, in AK1 ؊/؊ mice, fast-twitch gastrocnemius, but not slow-twitch soleus, had an increase in adenine nucleotide translocator (ANT) and mitochondrial creatine kinase protein, along with a doubling of the intermyofibrillar mitochondrial volume. These results provide molecular evidence for wide-scale remodeling in AK1-deficient muscles aimed at preservation of efficient energetic communication between ATP producing and utilizing cellular sites.

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Section: Discussionmentioning

confidence: 99%

Adenylate Kinase 1 Deficiency Induces Molecular and Structural Adaptations to Support Muscle Energy Metabolism

Janssen¹,

Groof²,

Wijers³

et al. 2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…The chemical displacements, expressed in parts per million (ppm) using sodium 2,2dimethyl-2-silapentane-5-sulfonate as the reference signal, allowed the identification of the metabolites, which were consistent with those described by Sánchez-Moreno et al (1992). In all cases, triplicates were made of each experiment.…”

Section: Methodsmentioning

confidence: 73%

“…Metabolite excretion -After the same treatment as described above, the supernatants were collected to determine the excreted metabolites by nuclear magnetic resonance spectroscopy ( 1 H-NMR) (Sánchez-Moreno et al 1992). The chemical displacements, expressed in parts per million (ppm) using sodium 2,2dimethyl-2-silapentane-5-sulfonate as the reference signal, allowed the identification of the metabolites, which were consistent with those described by Sánchez-Moreno et al (1992).…”

Section: Methodsmentioning

confidence: 80%

“…The reduction of glycine and succinate may signal an effect on the glyoxalate cycle operating in Phytomonas sp. (Sánchez-Moreno et al 1992), which may explain pyruvate as a new excreted metabolite, as occurs with SPV. The decline in acetate may also be due to direct impact on acetate synthetase, particularly SPVI, which interferes with protein synthesis.…”

Section: Discussionmentioning

confidence: 99%

“…Change in the excretion of different metabolites is of great use in elucidating the toxic activity of these compounds, since the catabolism of Phytomonas in general follows a pattern common among the species or isolates studied (Sánchez-Moreno et al 1992, Fernández-Becerra et al 1997. In the present work, the main metabolites excreted by P. characias were acetate, ethanol, succinate, and glycine, in accordance with previous results.…”

Section: Discussionmentioning

confidence: 99%

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