Characterization of Carbonic Anhydrase In Vivo Using Magnetic Resonance Spectroscopy

Tomar, Jyoti; Shen, Jun

doi:10.3390/ijms21072442

Cited by 5 publications

(5 citation statements)

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“…Equation ( 1) was justified by analyzing the full Bloch-McConnell equation for the carbon dioxidebicarbonate exchange system. 22 In vivo bicarbonate T 1 was found to be 9.6 s in the occipital lobe using the inversion-recovery null method without saturating carbon dioxide. 16 The in vivo spectrum sum was fitted using linear combination, a cubic spline baseline in the range of 177-184 ppm and an in-house-developed Lac1.…”

Section: Data Processing and Analysismentioning

confidence: 96%

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Carbonic anhydrase activity in the frontal lobe of human brain

Liu

Araneta

et al. 2021

NMR in Biomedicine

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Carbonic anhydrase (CA) plays an important role in many biological processes. Recent technological advances have demonstrated the feasibility of measuring CA activity in the occipital lobe of human subjects in vivo. In this work we report, for the first time, in vivo measurement of CA activity in the frontal lobe of human brain, where structural and function abnormalities are strongly associated with symptoms of major psychiatric disorders. Despite the much larger magnetic field distortion in the frontal lobe, the pseudo first-order bicarbonate dehydration rate constant was determined with high precision using in vivo 13 C magnetic resonance magnetization transfer spectroscopy following oral administration of [U-13 C 6 ]glucose. Nuclear Overhauser effect pulses were used to increase the signal-to-noise ratio; no proton decoupling was applied. The unidirectional dehydration rate constant of bicarbonate was found to be 0.26 ± 0.07 s −1 , which is not statistically different from the dehydration rate constant in the occipital lobe determined in our previous study, indicating that CA activity in the two brain regions is essentially indistinguishable. These results demonstrate the feasibility of characterizing CA activity in the frontal lobe for future psychiatric studies.

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Section: Data Processing and Analysismentioning

confidence: 96%

“…The pseudo first-order rate constant for the unidirectional dehydration reaction (H + + HCO 3 − ! CO 2 + H 2 O) was calculated using the following equation 22 :…”

Section: Data Processing and Analysismentioning

confidence: 99%

Carbonic anhydrase activity in the frontal lobe of human brain

Liu

Araneta

et al. 2021

NMR in Biomedicine

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“…The carbonic anhydrase (CA) family of metalloenzymes includes numerous isoforms and is responsible for catalyzing the conversion of carbon dioxide to bicarbonate anion [ 71 ]. Under hypoxic conditions, HIF-1 translocates to the nucleus and activates the expression of carbonic anhydrase IX (CAIX) [ 72 ].…”

Section: Hypoxic Secretomementioning

confidence: 99%

Role of the Hypoxic-Secretome in Seed and Soil Metastatic Preparation

Clemente-González

Carnero

2022

Cancers

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During tumor growth, the delivery of oxygen to cells is impaired due to aberrant or absent vasculature. This causes an adaptative response that activates the expression of genes that control several essential processes, such as glycolysis, neovascularization, immune suppression, and the cancer stemness phenotype, leading to increased metastasis and resistance to therapy. Hypoxic tumor cells also respond to an altered hypoxic microenvironment by secreting vesicles, factors, cytokines and nucleic acids that modify not only the immediate microenvironment but also organs at distant sites, allowing or facilitating the attachment and growth of tumor cells and contributing to metastasis. Hypoxia induces the release of molecules of different biochemical natures, either secreted or inside extracellular vesicles, and both tumor cells and stromal cells are involved in this process. The mechanisms by which these signals that can modify the premetastatic niche are sent from the primary tumor site include changes in the extracellular matrix, recruitment and activation of different stromal cells and immune or nonimmune cells, metabolic reprogramming, and molecular signaling network rewiring. In this review, we will discuss how hypoxia might alter the premetastatic niche through different signaling molecules.

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“…Карбоновые ангидразы (CA 1 , EC 4.2.1.1) -это повсеместно распространенные металлоферменты, встречающиеся как у прокариотов, так и эукариотов, кодируются семью эволюционно не связанными семействами генов. У млекопитающих было описано 16 изоферментов a-СА или белков, связанных с CA с различной каталитической активностью, субклеточной локализацией и распределением в тканях [3]. Существует пять цитозольных форм (CA I, CA II, CA III, CA VII и CA XIII), пять мембраносвязанных изозимов (CA IV, CA IX, CA XII, CA XIV и CA XV), две митохондриальные формы (CA VA и CA VB) и секретируемый фермент CA (CA VI).…”

Section: карбоангидразы и их ингибиторыunclassified

Carbonic Anhydrase Inhibitors in the Treatment of Glaucoma. Review. Part I

Курышева

2020

Oftalʹmologiâ

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Characterization of Carbonic Anhydrase In Vivo Using Magnetic Resonance Spectroscopy

Cited by 5 publications

References 100 publications

Carbonic anhydrase activity in the frontal lobe of human brain

Carbonic anhydrase activity in the frontal lobe of human brain

Role of the Hypoxic-Secretome in Seed and Soil Metastatic Preparation

Carbonic Anhydrase Inhibitors in the Treatment of Glaucoma. Review. Part I

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