Characterization of cardiac fatty‐acid‐binding protein from human placenta

Das, Tanya; Sa, Gaurisankar; Mukherjea, Manju

doi:10.1111/j.1432-1033.1993.tb17602.x

Cited by 23 publications

(10 citation statements)

References 42 publications

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“…In human placenta, two types of fattyacid-binding proteins (14.2 kDa and 15.3 kDa) were purified and their presence could be related to fetal development [40]. Moreover, in guinea pig, lipoprotein lipase increases by 11-fold during last ten days of gestation and seems to be implicated in fatty acid transfer between mother and fetus [6].…”

Section: Membrane Markersmentioning

confidence: 99%

“…However, Thomas et al [6] and Das et al [40] suggested that free fatty acids may be transferred across the placenta in the absence of a concentration gradient due to the action of cytosolic fatty-acid-binding protein in the placenta. In our study, we demonstrated a latency phase in the transport of linoleic acid bound to albumin by brush border membranes suggesting that linoleic acid transport is a complicated process including more than one step.…”

Section: Membrane Markersmentioning

confidence: 99%

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Linoleic Acid Transport by Human Placental Syncytiotrophoblast Membranes

Lafond¹,

Simoneau²,

Savard³

et al. 1994

European Journal of Biochemistry

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The placenta syncytiotrophoblast is the site of exchange of nutrients, lipids and minerals between the mother and the fetus. In order to characterize the transport of fatty acids by the placenta, we purified bipolar syncytiotrophoblast brush border and basal plasma membranes from human placenta. These purified brush border and basal plasma membranes enriched 3-fold and 22-fold, respectively, in sodiudpotassium-ATPase and 27-fold and 6-fold in alkaline phosphatase activity, compared with the placental homogenates. Fatty acid transport was performed at different fatty acid albumin ratios to evaluate the optimal uptake conditions. The maximal transport efficiency, for linoleic acid bound to albumin by sonication, was obtained with a 6 : 1 fatty acidalbumin ratio in brush border and basal plasma membranes. The linoleic acid transport observed with brush border membranes followed Michaelis-Menten kinetics, with a Michaelis constant of 7.89 ? 0.01 pM and a maximal incorporation rate of 30.80 ? 6.39 pmol . mg-' . min-I. Linoleic acid transport was very low in basal plasma membranes and we obtained a Michaelis constant of 0.95 2 0.01 yM and a maximal incorporation rate of 1.62 +-5.06 pmol . mg-' . mine '. In order to show that linoleic acid accumulated within brush border and plasma membrane vesicles, and to eliminate the possibility of a non-specific binding of fatty acid to these membranes, we demonstrated by an osmolarity experiment, the decrease of the linoleic acid transport in brush border and basal plasma membranes obtained in the presence of 455 pM essential fatty acid at 23°C for 180 min. The results presented in this study suggest that linoleic acid is transported significantly by syncytiotrophoblast brush border membranes and basal plasma membranes. Thus, it may represent a unidirectional transport from mother to fetus through the brush border membranes facing the mother, followed by transport at a slower rate through basal plasma membranes facing the fetus.Fatty acids are required for adequate fetal development and growth. These fatty acids can be synthesized by the placenta [I, 21 or can be provided by external sources including the maternal circulation. The amount of fatty acids transported by the placenta to the fetus varies between species [3]. In the ruminant and feline fetus, with epitheliochorial and hemoendothelial placentation, respectively, the amount of fatty acids transported by the placenta is less important than in species with hemochorial placentation (e.g. humans) [4-61. In a review article, Coleman has reported that fatty acid transport seems to be affected by maternal nutrition and stages of gestation [7]. Essential fatty acids are provided only by the maternal diet because the metabolic enzymes required for their synthesis are not present in fetuses of mammals [7, 81. In the last trimester of pregnancy, these essential fatty acids are very important for the growth and the development of the nervous system [9, lo], for the elaboration of cellular Correspondence to J. Lafond, Universite du...

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Section: Membrane Markersmentioning

confidence: 99%

Section: Membrane Markersmentioning

confidence: 99%

Linoleic Acid Transport by Human Placental Syncytiotrophoblast Membranes

Lafond¹,

Simoneau²,

Savard³

et al. 1994

European Journal of Biochemistry

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“…At least two FABP isoforms have been detected in the syncytial cytoplasm: liver fatty acid binding protein (L-FABP) and cardiac fatty acid binding protein (C-FABP) (25). To become available for the fetus, the fatty acids are thought to cross the basal membrane either through diffusion or with the assistance of FABPs (26).…”

mentioning

confidence: 99%

Gestational and hormonal regulation of human placental lipoprotein lipase

Magnusson-Olsson

Hamark

Ericsson

et al. 2006

Journal of Lipid Research

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The fetal demand for FFA increases as gestation proceeds, and LPL represents one potential mechanism for increasing placental lipid transport. We examined LPL activity and protein expression in first trimester and term human placenta. The LPL activity was 3-fold higher in term (n 5 7; P , 0.05) compared with first trimester (n 5 6) placentas. The LPL expression appeared lower in microvillous membrane from first trimester (n 5 2) compared with term (n 5 2) placentas. We incubated isolated placental villous fragments with a variety of effectors [GW 1929, estradiol, insulin, cortisol, epinephrine, insulin-like growth factor-1 (IGF-1), and tumor necrosis factor-a] for 1, 3, and 24 h to investigate potential regulatory mechanisms. Decreased LPL activity was observed after 24 h of incubation with estradiol (1 mg/ml), insulin, cortisol, and IGF-1 (n 5 12; P , 0.05). We observed an increase in LPL activity after 3 h of incubation with estradiol (20 ng/ml) or hyperglycemic medium plus insulin (n 5 7; P , 0.05).

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“…Other authors using self-made antibodies against heart-and liver-type FABP have revealed expression of those proteins in placentas within 5 to 30 weeks of gestation (18). The reason for this discrepancy in our specimens has yet to be elucidated.…”

mentioning

confidence: 58%

Effects of leptin on the expression of fatty acid-binding proteins in human placental cell cultures

et al. 2011

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Abstract. The present study aimed to evaluate the protein expression of the transmembrane translocase FAT/CD36 and cytoplasmic H-FABP and L-FABP in human trophoblast tissue and evaluate the effects of exogenous leptin upon differential expression of each biomolecule; consequently, it aimed to derive information regarding the effects of leptin upon the expression of proteins implicated in fatty acid metabolism. Protein and total RNA were isolated from 72 samples of trophoblast tissue obtained from chorionic villous sampling. Of these, 36 samples were evaluated for protein (supernatant and pellet fraction separated) and the other 36 for total RNA expression. For each subgroup of samples, 12 were treated immediately and 24 were cultured. Half of the cultured samples were treated with 10 ng/ ml exogenously added leptin and the other half were untreated. Western blotting and PCR techniques were used for the evaluation of biomolecule expression. Our results were obtained from samples at a mean gestational week of 12 +5 (n=72; min, 11 +0 ; max, 14 +1 gw; SD,0.89). In promptly treated samples we observed the presence of FAT/ CD36 protein and absence of cytoplasmic FABPs. In the latter, only mRNA transcription of H-FABP was noted. A cytoplasmic pool of FAT/CD36 was also noted in the supernatant fraction of proteins. For cultured samples, when leptin was added, a statistically significant increase in FAT/CD36 protein expression was observed (n=24, p<0.001; mean difference, 0.219; SD, 0.0315; CI, -0.284 to -0.154). In our study we demonstrated the protein and mRNA expression of biomolecules implicated in fatty acid metabolism in human placenta. A cytoplasmic pool of the transmembrane protein FAT/CD36 was noted. Leptin caused the increase in FAT/ CD36 protein expression in the cultured samples. Therefore, we conclude that leptin has an immediate effect and plays a role in lipid metabolism in human placenta.

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Characterization of cardiac fatty‐acid‐binding protein from human placenta

Cited by 23 publications

References 42 publications

Linoleic Acid Transport by Human Placental Syncytiotrophoblast Membranes

Linoleic Acid Transport by Human Placental Syncytiotrophoblast Membranes

Gestational and hormonal regulation of human placental lipoprotein lipase

Effects of leptin on the expression of fatty acid-binding proteins in human placental cell cultures

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