Characterization of CD45RO+ memory T lymphocytes in keloid disease

Chen, Zhenqi; Zhou, Lin; Won, Taeho; Gao, Zhen; Wu, Xiaoli; Lu, Liming

doi:10.1111/bjd.16173

Cited by 34 publications

(41 citation statements)

References 48 publications

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“…Among them, nonsuppressive Tregs express a lower level of CD25 and CTLA‐4 than suppressive Tregs . In the study by Chen et al ., decreased expression of both CD25 and CTLA‐4 on Foxp3 + CD8 – T cells was observed in keloids . This population might represent nonsuppressive Tregs, although additional studies will be needed to verify this as FoxP3 is induced following T‐cell activation …”

mentioning

confidence: 99%

“…Moreover, the pathogenic role of the IFN‐γ‐secreting CD8 − CD45RO + cells in keloid tissues will need to be investigated in more detail . Regardless, the study by Chen et al . implies that increased CD8 + T RM cells and decreased Tregs contribute to the inflammation in keloid tissues.…”

mentioning

confidence: 99%

“…In this issue, Chen et al . took advantage of flow cytometric analysis to investigate the subpopulations and functions of effector memory T cells in keloids and found that effector memory CD8 + T cells and CD103 + CD8 + resident memory T cells (T RM ) are increased in keloid tissues.…”

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confidence: 99%

“…Future studies will be required to determine exactly which CD8 – CD45RO + population is the major producer of interferon (IFN)‐γ in keloid tissues . Of the CD3 + CD8 − T‐cell populations, T‐helper 1 cells, natural killer T cells and γδ T cells all have the potential to produce IFN‐γ.…”

mentioning

confidence: 99%

“…Of the CD3 + CD8 − T‐cell populations, T‐helper 1 cells, natural killer T cells and γδ T cells all have the potential to produce IFN‐γ. Moreover, the pathogenic role of the IFN‐γ‐secreting CD8 − CD45RO + cells in keloid tissues will need to be investigated in more detail . Regardless, the study by Chen et al .…”

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confidence: 99%

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Further evidence for T-cell involvement in the pathogenesis of keloids

Kim

2018

British Journal of Dermatology

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Further evidence for T-cell involvement in the pathogenesis of keloids

Kim

2018

British Journal of Dermatology

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CTHRC1+ fibroblasts are stimulated by macrophage‐secreted SPP1 to induce excessive collagen deposition in keloids

Liu

Huang

Gong

et al. 2022

Clinical & Translational Med

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Editor, Fibrosis is characterized by fibroblast dysfunction and excessive deposition of cell-matrix that affect the normal functioning of the original tissue or organ. 1 The pathogenesis of keloids is complex and remains elusive. Multiple studies have suggested that skin color and tension, 2 immunity, 3 hormones, inflammatory stimulation, 4 genes 5 and other factors play critical roles in the onset and development of keloids. In addition to fibroblasts, immune cells that include macrophages, 6 mast cells 7 and lymphocytes 8 are also important to keloid pathogenesis via cytokine secretion and the triggering of abnormal signaling pathways. 9,10 Therefore, performing single-cell RNA-sEquation (scRNA-seq) that can identify heterogenous cell populations and cellular developmental pathways is thus valuable, as it will provide insight into the key pathogenic cell-types and the molecular patterns.In this study, we performed scRNA-seq on six pairs of keloid samples to elucidate the pathogenesis of skin fibrosis. Detailed methods and single-cell sequencing analysis are described in the Supplemental materials.We obtained lesion and non-lesion skin biopsies from six keloid patients (Figure 1A, Figure S1A,B, Table S1) and performed hematoxylin and eosin (H&E) and Masson staining (Figure 1B). Then we applied single-cell sequencing (scRNA-seq) to 12 skin samples that included both lesion and non-lesion areas (Figure 1C). After stringent quality-control procedures, 60,732 high-quality cells were obtained for further analysis. We first visualized all subgroups by uniform manifold approximation and projection (UMAP) and then determined the different cell types that included fibroblasts, keratinocytes, endothelial cells, T cells, pericytes, myeloid cells, mast cells, B cells, adipocytes, melanocytes and salivary gland cells (Figure 1C,D). All cell types were identified in each sample, and we observed minimal batch effects in our study (Figure S1C--F). Top-marked signatures for each cell typeThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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