Characterization of cell selectivity, physiological stability and endotoxin neutralization capabilities of α-helix-based peptide amphiphiles

Ma, Zhi; Wei, Dandan; Yan, Ping; Zhu, Xin; Shan, Anshan; Bi, Zhongpeng

doi:10.1016/j.biomaterials.2015.02.063

Cited by 100 publications

(104 citation statements)

References 53 publications

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“…2, Fow-3 and Fow-3(1-19) showed significant hemolytic activity in a dose-dependent manner. Fow-3 displayed stronger hemolytic activity than its derivatives, and its hemolysis reached 88.1% at the concentration of 256 M. Fow-3(1-19) also displayed higher hemolytic activity than the other three peptide derivatives, and 38.9% hemolysis was observed at the concentration of 256 M. Fow-3(1-15), Fow-3 (19)(20)(21)(22)(23)(24)(25)(26)(27), and Fow-3(1-15-20-27) showed no obvious hemolytic activity even at 256 M (less than 5% hemolysis), suggesting that the 4-amino-acid segment from position 16 to position 19 in the central link region is of vital importance in maintaining the hemolytic activity of Fow-3. Melittin, which is known to have strong antimicrobial activities against all bacteria, caused complete hemolysis at the concentrations of more than 32 m⌴.…”

Section: Resultsmentioning

confidence: 98%

“…The antimicrobial activity of the designed peptides was tested against three Gram-positive and five Gram-negative bacterial strains. As shown in Table 2, Fow-3 was potent against all strains, with MIC values in the range of 2 to 4 M. Fow-3(1-15), the analog containing only the N-terminal segment of Fow-3, exhibited poor efficacy against all the tested bacterial strains (MICs of Ն64 M), whereas no antimicrobial activity for tested microorganisms (Ͼ128 M) was observed for Fow-3(1-19) or Fow-3 (19)(20)(21)(22)(23)(24)(25)(26)(27). The MIC values of Fow-3 (1-15-20-27) for the tested bacteria are approximated to those of the parent peptide.…”

Section: Resultsmentioning

confidence: 98%

“…Fow-3(1-19) displayed the highest cytotoxic activity in the concentration range of 8 to 128 M. At the highest concentration of 256 M, the cell survival rates seen with Fow-3 and Fow-3(1-19) were less than 30%. Fow-3(1-15), Fow-3 (19)(20)(21)(22)(23)(24)(25)(26)(27), and Fow-3(1-15-20-27) displayed significantly lower cytotoxic activity on cells than Fow-3 and Fow-3(1-19). At the con- Stability of peptides.…”

Section: Resultsmentioning

confidence: 99%

“…Fow-3 is composed of 27 amino acid residues, with broad-spectrum antimicrobial activity but with significant hemolysis and cytotoxicity. To further reduce the cell toxicity of Fow-3 and explore its antibacterial mechanism, the derived peptides of Fow-3, Fow-3(1-15), Fow-3(1-19), Fow-3 (20)(21)(22)(23)(24)(25)(26)(27), and Fow-3(1-15-20-27) ( Table 1) were designed. The structure and molecular weight of the peptides were verified by ESI-MS.…”

Section: Resultsmentioning

confidence: 99%

“…As previously described (22), E. coli ATCC 25922 and S. aureus ATCC 29213 in exponential phase were diluted to an OD 600 of 0.2 with 10 mM PBS and incubated at 37°C for 1 h with 1ϫ MIC peptides. After incubation, the cells were centrifuged at 5,000 ϫ g for 5 min and washed 3 times with PBS.…”

Section: ) As Previously Described (19)mentioning

confidence: 99%

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The Central Hinge Link Truncation of the Antimicrobial Peptide Fowlicidin-3 Enhances Its Cell Selectivity without Antibacterial Activity Loss

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: ) As Previously Described (19)mentioning

confidence: 99%

See 3 more Smart Citations

The Central Hinge Link Truncation of the Antimicrobial Peptide Fowlicidin-3 Enhances Its Cell Selectivity without Antibacterial Activity Loss

Chen

et al. 2016

Antimicrob Agents Chemother

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Green Fabrication of Amphiphilic Quaternized β‐Chitin Derivatives with Excellent Biocompatibility and Antibacterial Activities for Wound Healing

et al. 2018

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Bacterial infection has always been a great threat to public health, and new antimicrobials to combat it are urgently needed. Here, a series of quaternized β-chitin derivatives is prepared simply and homogeneously in an aqueous KOH/urea solution, which is a high-efficiency, energy-saving, and "green" route for the modification of chitin. The mild reaction conditions keep the acetamido groups of β-chitin intact and introduce quaternary ammonium groups on the primary hydroxyl at the C-6 position of the chitin backbone, allowing the quaternized β-chitin derivatives (QCs) to easily form micelles. These QCs are found to exhibit excellent antimicrobial activities against Escherichia coli, Staphylococcus aureus, Candida albicans, and Rhizopus oryzae with minimum inhibitory concentrations (MICs) of 8, 12, 60, and 40 µg mL , respectively. As a specific highlight, their inherent outstanding biocompatibility and significant accelerating effects on the healing of uninfected, E. coli-infected, and S. aureus-infected wounds imply that these novel polysaccharide-based materials can be used as dressings for clinical skin regeneration, particularly for infected wounds.

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Specific Clearance of Lipopolysaccharide from Blood Based on Peptide Bottlebrush Polymer for Sepsis Therapy

Shi,

Zhang,

Yang

et al. 2023

Advanced Materials

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Lipopolysaccharide (LPS) is the primary bacterial toxin that is vital to the pathogenesis and progression of sepsis associated with extremely high morbidity and mortality worldwide. However, specific clearance of LPS from circulating blood is highly challenging because of the structural complexity and its variation between/within bacterial species. Herein, a robust strategy based on phage display screening and hemocompatible peptide bottlebrush polymer design for specific clearance of targeted LPS from circulating blood is proposed. Using LPS extracted from Escherichia coli as an example, a novel peptide (HWKAVNWLKPWT) with high affinity (KD < 1.0 nм), specificity, and neutralization activity (95.9 ± 0.1%) against the targeted LPS is discovered via iterative affinity selection coupled with endotoxin detoxification screening. A hemocompatible bottlebrush polymer bearing the short peptide [poly(PEGMEA‐co‐PEP‐1)] exhibits high LPS selectivity to reduce circulating LPS level from 2.63 ± 0.01 to 0.78 ± 0.05 EU mL−1 in sepsis rabbits via extracorporeal hemoperfusion (LPS clearance ratio > 70%), reversing the LPS‐induced leukocytopenia and multiple organ damages significantly. This work provides a universal paradigm for developing a highly selective hemoadsorbent library fully covering the LPS family, which is promising to create a new era of precision medicine in sepsis therapy.

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Characterization of cell selectivity, physiological stability and endotoxin neutralization capabilities of α-helix-based peptide amphiphiles

Cited by 100 publications

References 53 publications

The Central Hinge Link Truncation of the Antimicrobial Peptide Fowlicidin-3 Enhances Its Cell Selectivity without Antibacterial Activity Loss

The Central Hinge Link Truncation of the Antimicrobial Peptide Fowlicidin-3 Enhances Its Cell Selectivity without Antibacterial Activity Loss

Green Fabrication of Amphiphilic Quaternized β‐Chitin Derivatives with Excellent Biocompatibility and Antibacterial Activities for Wound Healing

Specific Clearance of Lipopolysaccharide from Blood Based on Peptide Bottlebrush Polymer for Sepsis Therapy

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