Characterization of Cellulase from Geotrichum candidum Strain Gad1 Approaching Bioethanol Production

Gad, A. M.; Suleiman, Waleed B.; El-Sheikh, Hussein H.; El-mezayen, Hatem A.; Beltagy, Ehab A.

doi:10.1007/s13369-021-06391-z

Cited by 22 publications

(13 citation statements)

References 49 publications

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“…LPMOs produced by G. candidum are promising candidates for enzymatic cocktails applied in biorefineries enrichment. Moreover, a cellulase isolated from G. candidum GAD1 was found to be promising for the degradation of carboxymethylcellulose salt from agricultural waste (rice straw), the fermentation of which resulted in bioethanol production [ 80 ].…”

Section: Production Of Enzymes With Industrial Interestmentioning

confidence: 99%

Uncovering the Biotechnological Importance of Geotrichum candidum

Kamilari

Stanton

Reen

et al. 2023

Foods

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Fungi make a fundamental contribution to several biotechnological processes, including brewing, winemaking, and the production of enzymes, organic acids, alcohols, antibiotics, and pharmaceuticals. The present review explores the biotechnological importance of the filamentous yeast-like fungus Geotrichum candidum, a ubiquitous species known for its use as a starter in the dairy industry. To uncover G. candidum’s biotechnological role, we performed a search for related work through the scientific indexing internet services, Web of Science and Google Scholar. The following query was used: Geotrichum candidum, producing about 6500 scientific papers from 2017 to 2022. From these, approximately 150 that were associated with industrial applications of G. candidum were selected. Our analysis revealed that apart from its role as a starter in the dairy and brewing industries, this species has been administered as a probiotic nutritional supplement in fish, indicating improvements in developmental and immunological parameters. Strains of this species produce a plethora of biotechnologically important enzymes, including cellulases, β-glucanases, xylanases, lipases, proteases, and α-amylases. Moreover, strains that produce antimicrobial compounds and that are capable of bioremediation were identified. The findings of the present review demonstrate the importance of G. candidum for agrifood- and bio-industries and provide further insights into its potential future biotechnological roles.

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Section: Production Of Enzymes With Industrial Interestmentioning

confidence: 99%

Uncovering the Biotechnological Importance of Geotrichum candidum

Kamilari

Stanton

Reen

et al. 2023

Foods

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“…Sampling was done using sterile polythene bags. Serial dilution method [32,33] was implemented on yeast extract malt extract medium [34], caseinstarch agar medium [35], and starch nitrate agar medium by 0.1 mL of serially diluted samples as an inoculum, followed by incubation at 30 °C for 7 days, and then submitted to further identi cation based on their morphological characteristics.…”

Section: Isolation Of Actinomycetes From Compost Samplesmentioning

confidence: 99%

Ecofriendly biosynthesis of Ag-NPs by Streptomyces griseus eradicating Candida albicans associated with different types of dysspermatism

El-Enain

Abed

Helal

et al. 2022

Preprint

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Silver nanoparticles Ag-NPs have potent biological properties such as antibacterial, antifungal, anti-viral and anti-inflammatory activities. A simple, eco-friendly, inexpensive method was conducted in order to synthesize silver nanoparticles by actinomycetes. A total of 17 actinomycetes isolates were recovered from serially diluted compost sample and subsequently examined for their capabilities to produce Ag-NPs. Alongside, Candida albicans was isolated from human semen that affect male infertility, and subsequently submitted to susceptibility test using the well diffusion method. It was found that the Ag-NPs biosynthesized by Streptomyces griseus Azhar.S2 have high zone of inhibition against Candida albicans which was recorded by measuring the diameter of inhibition zone that was 20.5 ± .05 mm. The biosynthesized Ag-NPs had been characterized using UV-vis spectroscopy, FTIR, EDX and TEM. Conclusively, Candida albicans isolated from different types of dysspermatism human semen was highly eradicated by microbially synthesized Ag-NPs from Streptomyces griseus Azhar.S2.

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“…Recently, there has been an increased interest in exploration of the Fungi kingdom for natural products due to the huge variety of species within this kingdom and the diverse habitats in which they are found [ 4 , 5 , 6 ]. The ability of fungi to colonize a wide range of habitats—from terrestrial to marine environments—grants them unique adaptive capabilities which underscore their potential to produce a vast repertoire of natural products with potential pharmaceutical and biotechnological applications [ 7 , 8 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Diversity and Biosynthetic Potential of Fungi Isolated from St. John’s Island, Singapore

Munusamy

Tan

Nge

et al. 2023

IJMS

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Adaptation to a wide variety of habitats allows fungi to develop unique abilities to produce diverse secondary metabolites with diverse bioactivities. In this study, 30 Ascomycetes fungi isolated from St. John’s Island, Singapore were investigated for their general biosynthetic potential and their ability to produce antimicrobial secondary metabolites (SMs). All the 30 fungal isolates belong to the Phylum Ascomycota and are distributed into 6 orders and 18 genera with Order Hypocreales having the highest number of representative (37%). Screening for polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) genes using degenerate PCR led to the identification of 23 polyketide synthases (PKSs) and 5 nonribosomal peptide synthetases (NRPSs) grouped into nine distinct clades based on their reduction capabilities. Some of the identified PKSs genes share high similarities between species and known reference genes, suggesting the possibility of conserved biosynthesis of closely related compounds from different fungi. Fungal extracts were tested for their antimicrobial activity against S. aureus, Methicillin-resistant S. aureus (MRSA), and Candida albicans. Bioassay-guided fractionation of the active constituents from two promising isolates resulted in the isolation of seven compounds: Penilumamides A, D, and E from strain F4335 and xanthomegnin, viomellein, pretrichodermamide C and vioxanthin from strain F7180. Vioxanthin exhibited the best antibacterial activity with IC50 values of 3.0 μM and 1.6 μM against S. aureus and MRSA respectively. Viomellein revealed weak antiproliferative activity against A549 cells with an IC50 of 42 μM. The results from this study give valuable insights into the diversity and biosynthetic potential of fungi from this unique habitat and forms a background for an in-depth analysis of the biosynthetic capability of selected strains of interest with the aim of discovering novel fungal natural products.

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Characterization of Cellulase from Geotrichum candidum Strain Gad1 Approaching Bioethanol Production

Cited by 22 publications

References 49 publications

Uncovering the Biotechnological Importance of Geotrichum candidum

Uncovering the Biotechnological Importance of Geotrichum candidum

Ecofriendly biosynthesis of Ag-NPs by Streptomyces griseus eradicating Candida albicans associated with different types of dysspermatism

Diversity and Biosynthetic Potential of Fungi Isolated from St. John’s Island, Singapore

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