Characterization of Certain Gram-Negative Bacteria from Surface Waters1

Abstract

HOADLEY, A. W. (University of Wisconsin, Madison), AND ELIZABETH MCCOY. Characterization of certain gram-negative bacteria from surface waters. Appl. Microbiol. 13:575-578. 1965.-Cultures of gram-negative bacteria with oxidative glucose metabolism were isolated from surface waters by a highly selective technique, and were classified into 11 types. The predominant type, making up about 50% of the isolated cultures, was cytochrome oxidase-positive, produced fluorescent pigment, and failed to grow at 37 C. A similar type, which differed in being cytochrome oxidase-negative, constituited about 10% of the isolates. Both types of bacteria probably were composed of more than one species. A third type, composed of purple-pigmented pseudomonads, made up approximately 30% of the isolated cultures, and probably represented the predominant species. Other bacterial types isolated constituted less than 10% of the cultures examined.

Pseudomonas Diversity Within Urban Freshwaters

“…As a whole, pseudomonads are often not abundant within freshwater environments (Newton et al, 2011). When pseudomonads are abundant within freshwaters, their source is typically associated with storm waters (Hoadley and McCoy, 1966; Oliveri et al, 1977; Selvakumar and Borst, 2006; Fisher et al, 2015). Culture-independent, 16S rRNA gene amplification surveys of freshwaters, rarely detect pseudomonads indicative of their absence/low abundance.…”

Comparison of Methods for Enumerating Fluorescent Bacteria1

“…The commonly accepted bacterial indicators of pollution, the total coliforms, fecal coliforms, fecal streptococci, and the standard plate count at 35 C, are more concerned with public health aspects of pollution than they are with the addition of nutrients to water. The fluorescent pseudomonads have been proposed as a group of bacteria that may respond to surface runoff from agricultural lands by increasing in numbers (2). Additionally, one component organism of the group, Pseudomonas aeruginosa, might also be an indication of fecal pollution (1, 2).…”