Characterization of CH₄-oxidizing and N₂O-reducing Bacterial Consortia Enriched from the Rhizospheres of Maize and Tall Fescue

Abstract

대표적인 non-CO 2 온실가스인 메탄(CH 4 )과 아산화질소이들 온실가스의 배출량을 최소화할 수 있 는 다양한 방법들에 대한 연구가 필요하다. CH 4 와 N 2 O 배 출을 저감할 수 있는 다양한 기술 중, 미생물을 이용한 생물 학적 저감 기술은 물리•화학적인 기술에 비해 경제적이고 환 경친화적 방법으로 주목을 받고 있다[3]. 대부분의 생물학적 CH 4 와 N 2 O 저감기술은 CH 4 를 CO 2 로 산화하거나, N 2 O를 N 2 로 환원할 수 있는 세균을 활용하고 있다[4, 5]. 세균에 의 한 CH 4 산화의 최종산물인 CO 2 도 온실가스이지만, CH 4 의 GWP가 CO 2 의 28배이므로, CH 4 을 1 mole을 제거하면 CO 2 를 28 mole 제거하는 효과가 있다. 비록 CH 4 산화 혹은 N 2 O 환원 세균에 관한 연구가 보고되고 있지만[5, 6], 혁신적인 신기술 개발을 위해서는 새로운 미생물자원 확보를 위한 연 구는 지속될 필요가 있다[7, 8].

Inoculation effect of Pseudomonas sp. TF716 on N 2 O emissions during rhizoremediation of diesel-contaminated soil

“…Greater N 2 O reduction with the addition of root exudate has also been reported in previous studies(Lee et al, 2021a, Henry et al, 2008. For example, when root exudate was added to N 2 O-reducing consortia, the N 2 O reduction rate was 1.3-2.7 times higher than without the exudate(Lee et al, 2021a). The root exudate of maize and tall fescue contains various organic compounds such as sugars, amino acids, organic acids, fatty acids, alcohols, and phosphate(Naveed et al, 2017, Valé et al, 2005, Liu et al, 2015.…”

“…has 2 O reduction rate for strain TF716 increased 1.5-fold in the presence of the root exudate, though there was no signi cant difference between the exudates for tall fescue (14.7±0.3 mmol N 2 O•g dry cells −1 •h −1 ) and maize (14.4±0.8 mmol N 2 O•g dry cells −1 •h −1 ). Greater N 2 O reduction with the addition of root exudate has also been reported in previous studies(Lee et al, 2021a, Henry et al, 2008. For example, when root exudate was added to N 2 O-reducing consortia, the N 2 O reduction rate was 1.3-2.7 times higher than without the exudate(Lee et al, 2021a).…”

“…The root exudate of tall fescue (F. arundinacea) and maize (Z. mays) was prepared in a similar manner to that described in a previous paper (Lee et al, 2021a). The COD of the root exudate for tall fescue and maize was 750 and 780 mg•L -1 , respectively.…”

“…The root exudate of maize and tall fescue contains various organic compounds such as sugars, amino acids, organic acids, fatty acids, alcohols, and phosphate(Naveed et al, 2017, Valé et al, 2005, Liu et al, 2015. It is assumed that these organic compounds were utilized as nutrients or growth-promotion factors for strain TF716, thus leading to a higher N 2 O reduction capacity(Lee et al, 2021a, Yan et al, 1996. reduction increased after the planting of the tall fescue.…”

Inoculation effect of Pseudomonas sp. TF716 on N2O emissions during rhizoremediation of diesel-contaminated soil

“…The root exudate of tall fescue ( F. arundinacea ) and maize ( Z. mays ) was prepared in a similar manner to that described in a previous paper 12 . Tall fescue and maize, grown in non-contaminated soil, was carefully sampled, and the soil adhering loosely to the root removed by shaking the plant.…”