Characterization of chemical composition of Agaricus brasiliensis polysaccharides and its effect on myocardial SOD activity, MDA and caspase-3 level in ischemia–reperfusion rats

Zhang, Song; He, Ben; Ge, Junbo; Zhai, Changlin; Liu, Xin; Liu, Pingang

doi:10.1016/j.ijbiomac.2010.01.008

Cited by 31 publications

(7 citation statements)

References 23 publications

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“…Thus, inhibition of lipid peroxidation may attenuate IR heart injury. [18] As shown in Table 1, in this study, a significant increase in serum SOD activity and a decrease in MDA level were evident in rabbits following IR.…”

Section: Discussionsupporting

confidence: 60%

Effect of trapidil in myocardial ischemia-reperfusion injury in rabbit

et al. 2014

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Objectives:To evaluate the cardioprotective effects of trapidil on myocardial ischemia-reperfusion injury (MIRI) in rabbits.Materials and Methods:Rabbits were subjected to 40 min of myocardial ischemia followed by 120 min of reperfusion. Blood for superoxide dismutase (SOD) and malondialdehyde (MDA) were estimated. At the end of reperfusion, the rabbits were sacrificed and the hearts were isolated for histological examination. An apoptotic index (AI) was determined using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end-labeling (TUNEL) method. The expression of apoptosis-related proteins Bax and Bcl-2 was analyzed using immunohistochemistry. Statistical analyses were performed by one-way analysis of variance (ANOVA), P < 0.05 considered statistically significantResults:Trapidil caused a significant (P < 0.05) increase in SOD activity, as decreased MDA levels and significantly (P < 0.05) reduced the expression of Bax as compared with the ischemia-reperfusion (IR) control group.Conclusion:Trapidil may attenuate the myocardial damage produced by IR injury and offer potential cardioprotective action.

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Section: Discussionsupporting

confidence: 60%

Effect of trapidil in myocardial ischemia-reperfusion injury in rabbit

et al. 2014

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“…As shown in Figure 1, we found that T waves of ECG in MI model rats were inversed after ligation. Enzymes and biochemical materials in plasma or tissue, such as CK, SOD, and MDA, are cited as important parameters in the assessment of MI [21–24]. On day 3, the level of CK in plasma revealed a significant elevation in the model group compared to the controls and the sham surgery group (Table 1), suggesting the success of the MI model after coronary artery ligation.…”

Section: Resultsmentioning

confidence: 99%

Metabonomic Strategy to the Evaluation of Chinese Medicine Compound Danshen Dripping Pills Interfering Myocardial Ischemia in Rats

Xue

Zou

Zheng

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Coronary heart disease (CHD) is one of the highest mortality diseases in the world. Traditional Chinese medicine compound Danshen dripping pills (CDDPs) have currently made a great achievement in treating CHD. However, the therapeutic mechanism of CDDP is often poorly interpreted. In this study, a GC-MS-based metabonomic study was conducted to assess the holistic efficacy of CDDP for myocardial infarction in male Sprague-Dawley rats, which were divided into the control group, the sham group, the model group, the control + CDDP group, and the model + CDDP, with CDDP at a dose of 107 mg/kg·d (equal to 1.8 mL/kg·d). The metabonomic findings demonstrated great differences of metabolic pattern among sham, model, and the model + CDDP in the orthogonal partial least squares discriminant analysis (OPLS-DA) models, which coordinated well with the assessment of plasma biochemistry and histopathological assay. Differentially expressed metabolites suggested that energy metabolism, glycolysis, and lipid metabolism might be disrupted by myocardial infarction. Both the potential metabolic biomarkers and the biochemical histopathological indices were regulated effectively by CDDP.

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“…Lu et al ( 19 ) revealed that the protective effect of aloe polysaccharide on cerebral ischemia may be due to the inhibition of neuronal cell apoptosis via downregulation of caspase-3 protein expression. Agaricus brasiliensis polysaccharides may affect malondialdehyde and superoxide dismutase activity, and caspase-3 level in IR rats, resulting in cardiovascular protection ( 20 ). The effect of polysaccharide peptide (PSP) on cerebral injury caused by IR remains unknown, even though substantial research and numerous studies of the neuroprotective effect of the polysaccharides have been performed.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of polysaccharide peptide on cerebral ischemia‑reperfusion injury in rats

Xing

et al. 2018

Mol Med Report

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In the present study, the protective effects and regulatory mechanism of polysaccharide peptide (PSP) were investigated in rats with cerebral ischemia-reperfusion (IR) injury. Neuroblastoma N2a cells were divided into five groups: Negative control; IR injury; PSP low dose treatment; PSP middle dose treatment; and PSP high dose treatment. In vitro, the cell viability was detected by an MTT assay. ELISA was performed to determine the activity of lactate dehydrogenase (LDH) and caspase-3. A cerebral IR injury model in vivo was established, and hematoxylin and eosin (H&E) staining, western blotting, neurological deficit score and cerebral infarction were assessed. The cell viability was markedly improved following treatment with PSP and the activity of LDH and caspase-3 was decreased following PSP administration (P<0.05). The in vivo studies determined that the neurological deficit score and cerebral infarction volume were reduced with the concentration of PSP increasing between 150 and 250 mg/kg. The H&E staining indicated that PSP was able to protect the nerve cells against the cerebral IR injury. In addition, PSP upregulated the decreased silent information regulator protein 1, peroxisome proliferator-activated receptor γ coactivator-1α and apoptosis regulator B-cell lymphoma 2 expression induced by cerebral IR injury. The protein expression level of caspase-3 and apoptosis regulator apoptosis regulator Bcl-2-like protein 4 was downregulated following PSP administration. These results suggested that PSP may improve nerve cell viability, enhance the neuroprotective role in cerebral IR injury and provide a novel approach for the treatment of cerebral IR injury.

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Characterization of chemical composition of Agaricus brasiliensis polysaccharides and its effect on myocardial SOD activity, MDA and caspase-3 level in ischemia–reperfusion rats

Cited by 31 publications

References 23 publications

Effect of trapidil in myocardial ischemia-reperfusion injury in rabbit

Effect of trapidil in myocardial ischemia-reperfusion injury in rabbit

Metabonomic Strategy to the Evaluation of Chinese Medicine Compound Danshen Dripping Pills Interfering Myocardial Ischemia in Rats

Protective effect of polysaccharide peptide on cerebral ischemia‑reperfusion injury in rats

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