Characterization of Chemical Composition of Bryophyllum pinnatum Leaf Ethyl Acetate Fraction

Abstract

Aims: To characterize the chemical composition of Bryophyllum pinnatum leaf ethyl acetate fraction.
 Methodology: Quantitative phytochemical composition was assessed using gas chromatography fitted with flame ionization detector (GC-FID), while chemical characterization was via gas chromatography-mass spectrophotometry (GC-MS) analysis. The mass spectra peaks were matched with those found in the National Institute of Standard and Technology (NIST) spectral database.
 Results: Results revealed a rich presence of proanthocyanin, rutin, quinine, flavan-3-ol, anthocyanin, lunamarin, sapogenin, phenol, flavonones, steroids, epicatechin, kaempferol, phytate, oxalate, resveratol, catechin, flavones, tannin, ribalinidine, naringin, and spartein in varying proportion in B. pinnatum leaves ethylacetate fractions. GC-MS analysis of the fractions revealed the presence of at least 50 constituents. The major constituents were Hexadecanoic acid, methyl ester (24.88%), 10,13-Octadecadienoic acid, methyl ester (29.69%), Tetracosanoic acid, methyl ester (7.84%), Methyl stearate (6.97%), cis-Methyl 11-eicosenoate (6.26%), Methyl 18-methylnonadecanoate (4.99%), Docosanoic acid, methyl ester (3.71%) and 4,7-Methano-1H-indene, octahydro- (2.43%).
 Conclusion: This rich array of chemical compounds presents B. pinnatum leaves as an important source of potential lead compounds with biological and pharmacological benefits and hence a potential candidate for drug discovery.

Chemical Characterization of Crude Phenolic Leaf Extract of Acalypha wilkesiana

“…They have been used as common agents for the treatment, management and prevention of many diseases in traditional setting, especially in Africa (Nwiloh et al,2016). About 80% of the rural population in the developing world relies on traditional medicines for their health care (Igwe et al,2021). This potential of plants have been ascribed to their rich content of a wide variety of secondary metabolites, such as tannins, saponins, alkaloids, flavonoids, etc (Nwiloh et al,2016).…”

“…GC-FID analysis revealed the presence of dihydrocytisine, ammodendrine, spartein, hydroxylupanine, ribalinidine, anthocyanin, flavone, flavonones, aphylidine, proanthocyanidin, isolupanine, narigenin, sapogenin, cardiac glycoside, tannin, and cyanogenic glycoside in varying proportions. GC-MS analysis identified 14 constituents with major ones being 2,6-Octadienal, 3,7-dimethyl-, (Z) Citral (14.88 %), citral 2,6-Octadienal, 3,7dimethyl-, (E) (16.9 %), cis-3-Hexenyl cis-3hexenoate (10.59 %), trans-2,7- 8,4).0(7,9)] undecane (11.69 %). This study has shown that crude phenolic leaf extract of A. wilkesiana contains pharmacologically active compounds which support its traditional use in the treatment of oxidative stress related diseases and hence a potential candidate for drug discovery.…”

Changes in Hepatic Function Indices in Ulcerated Rats Treated with Persea americana Seed and Bryophyllum pinnatum Leaf Ethyl Acetate Fraction

“…18,19 The chemical composition, hepatoprotective activity, anti-ulcer activity, in vitro and in vivo anti-inflammatory and free radical scavenging activities of the leaf, root has also been reported. 20,21,22,23…”

Ulcero-Protective Potential of Ethyl Acetate Fractions of Persea americana Seed and Bryophyllum pinnatum Leaf Binary Combinations in Indomethacin Induced Gastric Ulcer

“…"Chemical composition, hepatoprotective activity, antiinflammatory effects, anti-ulcer activity, and free radical scavenging activities have also been reported" [24][25][26][27].…”