Characterization of chemical constituents and rats metabolites of an alkaloidal extract of Alstonia scholaris leaves by liquid chromatography coupled with mass spectrometry

Cao, Jing; Shen, Hongmei; Wang, Qi; Qian, Yi; Guo, Hong-Cheng; Lou, Kai; Qiao, Xue; Guo, De‐An; Luo, Xiao‐Dong; Ye, Min

doi:10.1016/j.jchromb.2015.07.044

Cited by 24 publications

(13 citation statements)

References 16 publications

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“…The authors of this paper have investigated intensively the phytochemical constituents of the different parts of the plant [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26]. The chemical profile and metabolites of alkaloidal extract of leaves of A. scholaris indicate that scholaricine, 19-epischolaricine, vallesamine, and picrinine are the major indole alkaloids [27,28].…”

Section: Introductionmentioning

confidence: 99%

Acute and Chronic Toxicity of Indole Alkaloids from Leaves of Alstonia scholaris (L.) R. Br. in Mice and Rats

Zhao

Su²,

Shang

et al. 2020

Nat. Prod. Bioprospect.

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Alstonia scholaris (L.) R. Br. (Apocynaceae) is an evergreen tree that has been used to treat lung diseases. In this study, the toxicity profile of indole alkaloids from leaves of A. scholaris was investigated. In acute toxicity tests, mice were administered total alkaloids (TA) and five indole alkaloids. In a chronic toxicity test, rats were continuously administered TA (50, 100, and 300 mg/kg bw) for 13 weeks, followed by a 4-week recovery. A single administration of TA affected the behavior of mice, and at 12.8 g/kg bw, prone position, shortness of breath, wheezing, and convulsion were observed. The half-lethal dose (LD 50) in mice was 5.48 g/kg bw, almost 2740 times the clinical dose in humans. Among the five indole alkaloids, the maximum tolerance dose in mice ranged from 0.75 to 4 g/kg bw. The TA-treated rats did not die and showed no adverse effects or dose-dependent changes in weight or food and water consumption, despite fluctuations in hematological and biochemical parameters compared with historical data. Furthermore, both gross and histopathological observations revealed no abnormalities in any organ. With daily oral administration to rats, the non-observed-adverse-effect-level of TA was 100 mg/ kg bw. The results indicate that TA is safe for clinical use.

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Section: Introductionmentioning

confidence: 99%

Acute and Chronic Toxicity of Indole Alkaloids from Leaves of Alstonia scholaris (L.) R. Br. in Mice and Rats

Zhao

Su²,

Shang

et al. 2020

Nat. Prod. Bioprospect.

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“…Phytochemical constituents of different parts of the plant were investigated intensively by our group (Cai et al, 2010(Cai et al, , 2008a(Cai et al, , 2008b(Cai et al, , 2007Chen et al, 2016;Du et al, 2007aDu et al, , 2007bFeng et al, 2009Feng et al, , 2008Liu et al, 2015;Pan et al, 2016;Qin et al, 2015aQin et al, , 2015bXu et al, 2009;Yang et al, 2015aYang et al, , 2015bYang et al, , 2014aYang et al, , 2014bZhang et al, 2014;Zhou et al, 2005). Meanwhile, the chemical profiling and metabolites of alkaloidal extract of A. scholaris were reported (Cao et al, 2015), in which scholaricine, 19-epischolaricine, vallesamine, and picrinine were the major indole alkaloids of its leaf ( Fig. 1.…”

Section: Introductionmentioning

confidence: 99%

Effects of indole alkaloids from leaf of Alstonia scholaris on post-infectious cough in mice

Zhao

Yang

Shang

et al. 2018

Journal of Ethnopharmacology

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“…During recent years, metabolomic studies have been carried out worldwide by the scientific community to explore the metabolite profiles and metabolic pathways of different biofluids/herbal extracts (Liang et al 2010;Prasad and Singh 2010;Zhang, Saif et al 2010;Bai et al 2011;Giorgi et al 2013;Bony et al 2014;Han et al 2011;Kim et al 2011;Ye et al 2011;Shobha et al 2012;Zhang et al 2012;Li et al 2013;Liu et al 2013;Wang, Bai et al 2013;Bertol et al 2015;Cao et al 2015;Hagel et al 2015;Jia et al 2015;Li, Cai et al 2016;Meyer & Maurer 2015;Qiao et al 2015;Wu et al 2015;Zhang, Qia et al 2015).…”

Section: Metabolomics Application In Herbal Drug Metabolism and Discomentioning

confidence: 99%

“…In another study, chemical constituents of alkaloidal extract (from the leaves of Alstonia scholaris (AAS)) and their metabolism were evaluated in rats after oral administration (Cao et al 2015). Thirty-five alkaloids were characterized from AAS extract.…”

Section: Metabolomics Application In Herbal Drug Metabolism and Discomentioning

confidence: 99%

An overview of recent developments in metabolomics and proteomics – phytotherapic research perspectives

Mumtaz

Hamid

Akhtar

et al. 2017

Frontiers in Life Science

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In recent years, medicinal plants have gained much attention as potential source of bioactives for the development of novel herbal drugs for primary healthcare. However, phytotherapeutic mechanisms of action of phytomedicines need to be explored comprehensively. Out of the available strategies for the said purpose, metabolomics is one, which results in the production of inclusive metabolite profiles providing a clear understanding of diagnostic changes in the levels of metabolites, leading to therapeutic monitoring of drug targets through the elucidation of metabolomic pathways. On the other hand, proteomics is also a powerful strategy to deal with systematic protein expression analysis and analyze different biomarkers compared to metabolomics during drug treatment. Currently, 1 H nuclear magnetic resonance spectroscopy, mass spectrometry (MS), Fourier transform infrared spectroscopy, gas chromatography equipped with mass spectrometry, liquid chromatography coupled with MS and ultra-performance liquid chromatography-MS coupled with multivariate statistical techniques, such as principle component analysis, partial least square and orthogonalpartial least square are among the widely applicable analytical tools for metabolite profiling, whereas two-dimensional electrophoresis and matrix-assisted laser desorption ionization time-of-flight-MS are the most accepted analytical methods for proteomic biomarker investigation. The present review summarizes the recent developments and perspectives of the biomarker investigation strategies so as to elaborate their imperative role for sustainable herbal drug developments.

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Characterization of chemical constituents and rats metabolites of an alkaloidal extract of Alstonia scholaris leaves by liquid chromatography coupled with mass spectrometry

Cited by 24 publications

References 16 publications

Acute and Chronic Toxicity of Indole Alkaloids from Leaves of Alstonia scholaris (L.) R. Br. in Mice and Rats

Acute and Chronic Toxicity of Indole Alkaloids from Leaves of Alstonia scholaris (L.) R. Br. in Mice and Rats

Effects of indole alkaloids from leaf of Alstonia scholaris on post-infectious cough in mice

An overview of recent developments in metabolomics and proteomics – phytotherapic research perspectives

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