Characterization of chemotaxis and motility response towards fructose in Escherichia coli

Liu, Fanghai; Wang, Fangbin; Liu, Jian

doi:10.1016/j.bbrc.2020.04.087

Cited by 3 publications

(3 citation statements)

References 31 publications

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“…To verify this conjecture, we carried out swimming motility assays, and found that the diameter of the swimming zone of the degS mutant on motility plates was significantly smaller than that of the WT strain ( Figure 1B ; Supplementary Figure 1 ). Subsequently, we performed a chemotaxis assay using three widely utilized attractants, namely 50 mM fructose ( Liu et al, 2020b ), 100 mM serine ( l -ser; Roggo et al, 2018 ), and 100 μM aspartic acid ( l -Asp; Long et al, 2017 ). Either with or without attractant, the motility of the ΔdegS strain was found to be lower than that of the WT strain.…”

Section: Resultsmentioning

confidence: 99%

DegS protease regulates the motility, chemotaxis, and colonization of Vibrio cholerae

et al. 2023

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In bacteria, DegS protease functions as an activating factor of the σE envelope stress response system, which ultimately activates the transcription of stress response genes in the cytoplasm. On the basis of high-throughput RNA sequencing, we have previously found that degS knockout inhibits the expression of flagellum synthesis- and chemotaxis-related genes, thereby indicating that DegS may be involved in the regulation of V. cholerae motility. In this study, we examined the relationships between DegS and motility in V. cholerae. Swimming motility and chemotaxis assays revealed that degS or rpoE deletion promotes a substantial reduction in the motility and chemotaxis of V. cholerae, whereas these activities were restored in ΔdegS::degS and ΔdegSΔrseA strains, indicating that DegS is partially dependent on σE to positively regulate V. cholerae activity. Gene-act network analysis revealed that the cAMP–CRP–RpoS signaling pathway, which plays an important role in flagellar synthesis, is significantly inhibited in ΔdegS mutants, whereas in response to the overexpression of cyaA/crp and rpoS in the ΔdegS strain, the motility and chemotaxis of the ΔdegS + cyaA/crp and ΔdegS + rpoS strains were partially restored compared with the ΔdegS strain. We further demonstrated that transcription levels of the flagellar regulatory gene flhF are regulated by DegS via the cAMP–CRP–RpoS signaling pathway. Overexpression of the flhF gene in the ΔdegS strain partially restored motility and chemotaxis. In addition, suckling mouse intestinal colonization experiments indicated that the ΔdegS and ΔrpoE strains were characterized by the poor colonization of mouse intestines, whereas colonization efficacy was restored in the ΔdegSΔrseA, ΔdegS + cyaA/crp, ΔdegS + rpoS, and ΔdegS + flhF strains. Collectively, our findings indicate that DegS regulates the motility and chemotaxis of V. cholerae via the cAMP–CRP–RpoS–FlhF pathway, thereby influencing the colonization of suckling mouse intestines.

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Section: Resultsmentioning

confidence: 99%

DegS protease regulates the motility, chemotaxis, and colonization of Vibrio cholerae

et al. 2023

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“…For cells cultured with different concentration of l ‐fucose (0.1, 1, 10, and 50 mM), the probability density of cells near the surface gradually increased with the concentration of l ‐fucose (Figure 4), suggesting that l ‐fucose promotes bacterial surface aggregation. Recent studies showed that the decrease of motor rotation speed would further promote the accumulation of bacteria near the surface [28,29]. Combined with the result above that the flagellar motor rotation speed of E. coli cultured with l ‐fucose reduced, the results indicate the l ‐fucose promotes bacterial surface aggregation by decreasing the motor rotation speed of bacteria.…”

Section: Resultsmentioning

confidence: 83%

“…the '-' in the box represents the median value, and the whiskers represents the data range of the 25th and 75th percentile probability density of cells near the surface gradually increased with the concentration of L-fucose (Figure 4), suggesting that L-fucose promotes bacterial surface aggregation. Recent studies showed that the decrease of motor rotation speed would further promote the accumulation of bacteria near the surface [28,29].…”

Section: L-fucose Promotes Bacterial Surface Aggregation and Adhesionmentioning

confidence: 99%

Characterization of the response of Escherichia coli tol‐fucose in bacterial swimming motility

Chen

Wang

et al. 2022

J Basic Microbiol

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L-Fucose, as a monosaccharide in nature, plays a crucial role in bacteria colonization. Escherichia coli (E. coli), as a common microorganism in environment, utilize bacterial flagellar motor to drive the rotation of flagella, which is regulated by chemotactic signal transduction signals. Yet the effect of L-fucose to bacterial motility remains unclear. The effect of L-fucose on the swimming motility of bacteria was investigated from the level of single flagellar motor to individual cell and cell population by employing a bead assay, a high-throughput 2D tracking assay and a high-throughput dark-field flicker microscopy. The results showed that the swimming motility of the bacteria cultured with L-fucose was decreased, while the tumble frequency increased. Furthermore, the behavioral alterations of bacteria affected by L-fucose were directly reveled by measuring the cell distribution of bacteria swimming near surfaces and bacterial surface adhesion, suggesting that L-fucose promotes bacterial surface aggregation and surface adhesion. The effect of L-fucose on bacterial swimming motility characterized in this study are consistent with the key role that L-fucose plays in bacterial colonization.

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The sports performance improving effects of konjac glucomannan with varying molecular weights in overtrained mice

Mao,

Wang,

Yuan

et al. 2024

International Journal of Biological Macromolecules

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Characterization of chemotaxis and motility response towards fructose in Escherichia coli

Cited by 3 publications

References 31 publications

DegS protease regulates the motility, chemotaxis, and colonization of Vibrio cholerae

DegS protease regulates the motility, chemotaxis, and colonization of Vibrio cholerae

Characterization of the response of Escherichia coli tol‐fucose in bacterial swimming motility

The sports performance improving effects of konjac glucomannan with varying molecular weights in overtrained mice

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