Characterization of chicken natural resistance-associated macrophage protein encoding genes (Nramp1 and Nramp2) and association with salmonellosis resistance

Abstract

ABSTRACT. Natural resistance-associated macrophage protein 1 and 2 encoding genes (Nramp1 and Nramp2) are related to many diseases. We cloned the cDNA of chicken Nramp1 and Nramp2 genes, characterized their expression and polymorphisms, and investigated the association of some SNPs with resistance to salmonellosis. The Nramp1 cDNA was 1746 bp long and the Nramp2 cDNA was 1938 bp long. These cDNAs are similar to previously reported cDNAs, varying by two and one amino acids, respectively. The chicken Nramp1 gene expressed predominantly in liver, thymus and spleen in both females and males. The Nramp2 gene expressed in almost all tissues, but ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (1): 618-630 (2013) Chicken Nramp1 and Nramp2 gene characterization 619 predominantly in breast muscle, leg muscle, cerebrum, cerebellum, lung, kidney, and heart in both females and males. We identified 45 SNPs and 2 indels in the chicken Nramp1 gene; three of 13 SNPs in the exons were missense mutations (Arg223Gln, Ala273Glu and Arg497Gln). Association analysis indicated that A24101991G is significantly associated with chicken salmonellosis resistance. These results will be useful for functional investigation of chicken Nramp1 and Nramp2 genes.

Polymorphism, Expression of Natural Resistance-associated Macrophage Protein 1 Encoding Gene (<i>NRAMP1</i>) and Its Association with Immune Traits in Pigs

“…The levels of NRAMP1 expressed in liver, spleen and lymphoid were higher, comparatively, while the levels in brain and kidney were lower. NRAMP1 gene expression has been observed by RT-PCR in different tissues from chickens where higher expression levels were found in liver, spleen, thymus and much lower expression levels in brain, kidney, which is consistent with our results in pigs (He et al, 2013). …”

Characterisation of subpopulations of chicken mononuclear phagocytes that express TIM4 and the macrophage colony-stimulating factor receptor (CSF1R)

“…The chicken SCL11A1 gene was previously shown to be expressed in liver, thymus and spleen. As in mice, SCL11A1 polymorphism was associated with resistance to Salmonellosis (57). Consistent with that report, SLC11A1 was undetectable in bursal cell populations (Table S1).…”

Nramp1 gene expression in different tissues of Meishan piglets from newborn to weaning

“…It serves an apparent role in host defense against microbial infection (Blackwell, 1996;Govoni and Gros, 1998;He et al, 2013), and is considered an important immune response-related gene (Wu et al, 2008;Chen et al, 2012;Badawy et al, 2013). Previous studies have indicated that the Nramp1 protein serves a role in pathogenic infection resistance via a mechanism involving the transport of metal ions such as Mn 2+ and Fe…”

“…In addition, another study found that the Nramp1 gene could influence Bacillus CalmetteGuérin (BCG) tuberculosis vaccine efficacy, indicating that Nramp1 plays an important immune regulation function with regard to pathogenic response (Badawy et al, 2013). Similarly, He et al (2013) found that A24101991G of Nramp1 was significantly associated with salmonellosis resistance.…”