Characterization of Chitinase Produced by the Alkaliphilic Bacillus mannanilyticus IB-OR17 B1 Strain

Production and Partial Purification of Thermostable Chitinase from Bacillus cereus BSH-4 and its Antifungal Activity

“…In contrast, Fe and Mg in (Ghorbel-Bellaaj et al, 2012) and Mg, Ca, and Cu in (Bouacem et al, 2018) have increased the relative chitinase activity. In (Aktuganov et al, 2018), Ca and Mg neither showed any significant inhibitory or activating effects on the enzyme. Enzyme inhibition by Zn may be associated with residues of acids like aspartic and glutamic acids left in chitinase.…”

“…Hence, changes in the pH value of the medium affect enzyme stability and activity as well. This could be due to the characteristic poly-extremophiles of bacteria present in particular ecosystems (Aktuganov et al, 2018). Metal ions function as links to both the substrate and the enzyme and the substrate, binding to them both thus stimulating enzyme activity by keeping the substrate in the active site of the enzyme (Shivakumar et al, 2014).…”

“…Chitinases are produced by bacteria, fungi, actinomycetes, higher plants, lower animals, and birds (Ghorbel-Bellaaj et al, 2012;Lestari et al, 2017;Cheba et al, 2018). Carbon and nitrogen are sourced from chitin by microorganisms (Lestari et al, 2017;Aktuganov et al, 2018). Microorganisms produce chitinase because they use chitin as an energy source, while fungi and insects produce chitinase because they are involved in morphogenesis (Korany et al, 2019).…”

“…The main genus of this group is Streptomyces; some chitinases are produced by several species such as S. aureofaciens, and S. olivaceoviridis for resourceful degradation of chitin. Other actinomycetes that produce chitinase include Actinoplanes, Micromonospora, and Nocardia (Aktuganov et al, 2018;Cheba et al, 2018;Ray et al, 2019;Akeed et al, 2020).…”