Characterization of Chlorella sorokiniana and Chlorella vulgaris fatty acid components under a wide range of light intensity and growth temperature for their use as biological resources

Abstract

This study aims to characterize the potential of three strains of microalgal species ( Chlorella sorokiniana KNUA114 and KNUA122; C. vulgaris KNUA104) for use as feedstock, based on their fatty acid compositions. Each strain was molecularly identified using four marker genes ( ITS , SSU , rbcL , and tufA ) and phylogenetically characterized. C. sorokiniana and C. vulgaris collected from Ulleung Island, South Korea, were homologous with other known species groups. Samples' fatty acid components were measured using GC/MS analysis in growth temperatures of 10 °C, 25 °C, and 35 °C. The growth rate of C. sorokiniana strains was higher than that of C. vulgaris under high-temperature conditions, confirming the potential industrial applicability of the former as feedstock material. Additionally, saturated fatty acid contents and productivities increased as biological resources of the C. sorokiniana strains were higher than those of C. vulgaris under high light intensity and temperature conditions. These results suggest that the fatty acid components of C. sorokiniana strains may potentially be used as biological resources (e.g., feedstock).

Effect of Different Cultivation Modes (Photoautotrophic, Mixotrophic, and Heterotrophic) on the Growth of Chlorella sp. and Biocompositions

“…Previous studies have determined the optimal growth temperature conditions for Chlorella vulgaris and Chlorella sorokiniana (Yun et al, 2020). Furthermore, it was found that an organic carbon source in a heterotrophic condition could be utilized by Chlorella (Kim et al, 2020).…”

Bioprospecting wild South African microalgae as a potential third-generation biofuel feedstock, biological carbon-capture agent and for nutraceutical applications

“…1a and b with some key observed features of UJEAS4 (Fig. 1a) as having spherical shaped cells with no flagella and the average sizes varied between 3 and 4 and 5 and 6 µm, which is a common feature of the genus Chlorella [4,39,40].…”

“…Saturated fatty acids (SFAs) have been described in numerous studies as biological tools for biodiesel production and microalgae are rich sources of these compounds [4]. The percentage of SFA content for C. sorokiniana and T. reginae corresponds to 35.73 ± 1.13% and 29.79 ± 1.06%, respectively.…”

“…Studies have shown that when compared to higher plants, algae have one of the most efficient photosynthetic and biomass productivity rates [1,2], and this results in an elevated biomass build-up. Other studies also reported that most microalgal species have great potential and value as bio-resources and climate mitigation agents [3,4]. To determine optimal use for more than 1,000,000 microalgal species, on-going investigations are conducted globally to analyse their primary metabolites (proteins, carbohydrates, lipids, fatty acids, amino acids, vitamins and sterols) and useful secondary metabolites (including allelochemicals, toxins and hormones) [5][6][7].…”

Effects of Co-culture on Improved Productivity and Bioresource for Microalgal Biomass Using the Floc-Forming Bacteria Melaminivora Jejuensis

“…Three flocculation bacterial strains (M. jejuensis KCTC 32230, C. flocculans KCTC 62943, and E. coli, dh5α) and the microalgal strain C. sorokiniana KNUA114 were prepared. C. sorokiniana KNUA114 was isolated from Okcheon Stream in Ulleung, Korea (Okcheon Stream, Ulleungeup, Ulleung-gun, Gyeongsangbuk-do; 37 Yun et al, 2020). The bacterial and algal strains were maintained at 30 • C on R2A agar plates composed of the following: proteose peptone, 0.5 g/L; casamino acid, 0.5 g/L; yeast extract, 0.5 g/L; dextrose, 0.5 g/L; soluble starch, 0.5 g/L; sodium pyruvate, 0.3 g/L; K 2 HPO 4 , 0.3 g/L; and MgSO 4 •7H 2 O, 0.05 g/L.…”