Characterization of Ciprofloxacin-Resistant and Ciprofloxacin-Susceptible Uropathogenic Escherichia coli Obtained from Patients with Gynecological Cancer

Abstract

The objective of this work was to assess the genetic characteristics of uropathogenic Escherichia coli, ciprofloxacin resistance or susceptibility, obtained from patients with gynecological cancer and urinary tract infection (UTI). Seventy-seven E. coli ciprofloxacin-resistant isolates and 38 ciprofloxacin-susceptible were analyzed by polymerase chain reaction (PCR) to determine the phylogenetic groups, virulence factors as iucC, fyuA, hlyC, cnf1 genes, and pks pathogenicity island. The presence of genes related to ciprofloxacin resistance such as qnrA, qnrB, qnrS, aac(6')-Ib-cr, and qepA, and the sequencing of DNA gyrase genes and topoisomerase IV were determined. The genetic profile of the isolates was determined by pulsed-field gel electrophoresis (PFGE). Statistical analysis was performed using Fisher's exact test and Chi-square test. Phylogenetic group B2 was the most prevalent although a great genetic diversity was observed by PFGE. Only genes associated to siderophores were found in ciprofloxacin-resistant isolates; however, in ciprofloxacin-susceptible isolates, genes related to siderophores and toxin, were detected. Additionally qnrB was detected in both populations, ciprofloxacin resistant and susceptible. DNA mutations in gyrA were Ser-83-Leu and Asp-87-Asn and in parC were Ser-80-Ile and Glu-84-Val, Glu-84-Lys. In conclusion, it was observed a high prevalence of qnrB in the population studied; in addition, it was the first time the pks island was observed only in ciprofloxacin-susceptible isolates.

Occurrence of Carriage of Multidrug Resistant Enterobacteriaceae among Pregnant Women in the Primary Health Center and Hospital Setting in Surabaya, Indonesia

“…40,41 These results suggest the consequence of horizontal transfer of antimicrobial-resistant genes between strains rather than the spread of specific bacterial clones. 42 In conclusion, we investigated the prevalence of multidrug resistant Enterobacteriaceae carriage in pregnant women in the PHC and hospital settings. Our results revealed a high carriage rate of ESBL-EC in pregnant women in Surabaya, Indonesia.…”

Urine flora imbalance and new biomarkers in prostate cancer and benign prostatic hyperplasia

“…Moreover, Escherichia coli induces persistent colonization, exacerbate inflammation and trigger carcinogenesis (27). Escherichia coli is a crucial factor for the development of colorectal cancer, breast cancer, gynecological cancer, and prostate cancer (28)(29)(30)(31). The enrichment in Escherichia coli contributes to host cell inflammatory responses and intestinal dysbiosis, promoting the progression of inflammatory bowel disease (IBD) and CRC (28).…”