Characterization of cochlear nucleus principal cells of Meriones unguiculatus and Monodelphis domestica by use of calcium-binding protein immunolabeling

Bazwinsky, Ivonne; Härtig, Wolfgang; Rübsamen, Rudolf

doi:10.1016/j.jchemneu.2007.10.003

Cited by 38 publications

(35 citation statements)

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“…This pattern suggests that CR may define an identifiable subpopulation, as appears to be the case in the mammalian cochlear nucleus (see "DISCUSSION") (Bazwinsky et al 2008;Fredrich et al 2009;Lohmann and Friauf 1996). To better define the CR subpopulation in NA, we investigated the expression of Kv1.1 in NA using double immunofluorescent labeling of anti-Kv1.1 along with anti-MAP2 (Fig.…”

Section: Inverse Relationship Between Cr and Neun Expressionmentioning

confidence: 86%

“…While calretinin was the only CaBP found in the chick auditory brain stem nuclei (Parks et al 1997;Rogers 1989a), three members of the CaBPs family, calretinin, parvalbumin, and calbindin, have been found in the mammalian auditory system (Bazwinsky et al 2005(Bazwinsky et al , 2008Braun et al 1991b;Braun and Piepenstock 1993;Caicedo et al 1996Caicedo et al , 1997Celio 1990;Förster and Illing 2000;Fredrich et al 2009;Friauf 1993Friauf , 1994Idrizbegovic et al 2001Idrizbegovic et al , 2004Idrizbegovic et al , 2006Kelley et al 1992;Kulesza 2014;Logerot et al 2011;Lohmann and Friauf 1996;Matsubara 1990;Vater and Braun 1994). These CaBPs formed complementary patterns of expression in the cochlear nuclei (Bazwinsky et al 2008;Fredrich et al 2009;Lohmann and Friauf 1996).…”

Section: Comparison With Mammalian Auditory Brain Stem Expressionmentioning

confidence: 99%

“…These CaBPs formed complementary patterns of expression in the cochlear nuclei (Bazwinsky et al 2008;Fredrich et al 2009;Lohmann and Friauf 1996).…”

Section: Comparison With Mammalian Auditory Brain Stem Expressionmentioning

confidence: 99%

“…Heterogeneity in CaBP expression in the auditory brain stem has been studied in detail in several mammals. Using double immunofluorescence labeling Bazwinsky et al (2008) found that calbindin and calretinin were expressed in distinct subpopulations of globular bushy cells and octopus cells in the ventral cochlear nucleus of the gerbils and possum. Similarly, a study in rat brain stem showed that multipolar type I neurons in ventral cochlear nucleus expressed either PV or CR (Fredrich et al 2009).…”

Section: Comparison With Mammalian Auditory Brain Stem Expressionmentioning

confidence: 99%

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Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

Bloom

Williams

MacLeod

2014

JARO

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Multiple calcium-binding proteins (CaBPs) are expressed at high levels and in complementary patterns in the auditory pathways of birds, mammals, and other vertebrates, but whether specific members of the CaBP family can be used to identify neuronal subpopulations is unclear. We used double immunofluorescence labeling of calretinin (CR) in combination with neuronal markers to investigate the distribution of CR-expressing neurons in brainstem sections of the cochlear nucleus in the chicken (Gallus gallus domesticus). While CR was homogeneously expressed in cochlear nucleus magnocellularis, CR expression was highly heterogeneous in cochlear nucleus angularis (NA), a nucleus with diverse cell types analogous in function to neurons in the mammalian ventral cochlear nucleus. To quantify the distribution of CR in the total NA cell population, we used antibodies against neuronal nuclear protein (NeuN), a postmitotic neuronspecific nuclear marker. In NA neurons, NeuN label was variably localized to the cell nucleus and the cytoplasm, and the intensity of NeuN immunoreactivity was inversely correlated with the intensity of CR immunoreactivity. The percentage of CR+neurons in NA increased from 31 % in embryonic (E)17/18 chicks, to 44 % around hatching (E21), to 51 % in postnatal day (P) 8 chicks. By P8, the distribution of CR + neurons was uniform, both rostrocaudal and in the tonotopic (dorsoventral) axis.Immunoreactivity for the voltage-gated potassium ion channel Kv1.1, used as a marker for physiological type, showed broad and heterogeneous postsynaptic expression in NA, but did not correlate with CR expression. These results suggest that CR may define a subpopulation of neurons within nucleus angularis.

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Section: Inverse Relationship Between Cr and Neun Expressionmentioning

confidence: 86%

Section: Comparison With Mammalian Auditory Brain Stem Expressionmentioning

confidence: 99%

“…These CaBPs formed complementary patterns of expression in the cochlear nuclei (Bazwinsky et al 2008;Fredrich et al 2009;Lohmann and Friauf 1996).…”

Section: Comparison With Mammalian Auditory Brain Stem Expressionmentioning

confidence: 99%

Section: Comparison With Mammalian Auditory Brain Stem Expressionmentioning

confidence: 99%

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Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

Bloom

Williams

MacLeod

2014

JARO

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“…Both turtle and gecko NM and NA neurons express both CR and CB, although the two are differently distributed within the cell body in gecko. Similarly, both avian NM (Hack et al, 2000) and mammalian globular bushy cells are characterized by high levels of CR, whereas globular bushy cells express CR and PV in their cytoplasm, and octopus cells express CR and CB (Bazwinsky et al, 2008;Pór et al, 2005). These abundant and often complementary calcium-binding protein distributions suggest a requirement for calcium regulation in these auditory brainstem neurons, all of which are characterized by high rates of activity, phaselocked responses to auditory stimuli and calcium-permeable AMPA receptors (ChristensenDalsgaard et al, 2007;Eatock et al, 1981;Otis et al, 1995;Raman and Trussell, 1995;Trussell, 1999Trussell, , 2008.…”

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confidence: 99%

Calcium‐binding protein immunoreactivity characterizes the auditory system of Gekko gecko

Yang

Tang

Carr

2010

J of Comparative Neurology

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Geckos use vocalizations for intraspecific communication, but little is known about the organization of their central auditory system. We therefore used antibodies against the calciumbinding proteins calretinin (CR), parvalbumin (PV), and calbindin-D28k (CB) to characterize the gecko auditory system. We also examined expression of both glutamic acid decarboxlase (GAD) and synaptic vesicle protein (SV2). Western blots showed that these antibodies are specific to gecko brain. All three calcium-binding proteins were expressed in the auditory nerve, and CR immunoreactivity labeled the first-order nuclei and delineated the terminal fields associated with the ascending projections from the first-order auditory nuclei. PV expression characterized the superior olivary nuclei, whereas GAD immunoreactivity characterized many neurons in the nucleus of the lateral lemniscus and some neurons in the torus semicircularis. In the auditory midbrain, the distribution of CR, PV, and CB characterized divisions within the central nucleus of the torus semicircularis. All three calcium-binding proteins were expressed in nucleus medialis of the thalamus. These expression patterns are similar to those described for other vertebrates.Keywords cochlear nucleus; magnocellularis; laminaris; angularis; torus Over 30 years have passed since Foster and Hall (1978) reported that the ascending auditory pathways of the iguana resemble both the mammalian and the avian auditory pathways, from the level of the first-order neurons in the VIIIth nerve to the telencephalon. They recognized a basic plan for the organization of the auditory system in terrestrial vertebrates but also pointed out that the auditory capacities of the lizards were largely unknown. We now know more about the peripheral (Manley, 1990;Wever, 1978) and central (for reviews see Carr, 1992;Grothe et al., 2005;ten Donkelaar et al., 1987) auditory systems of lizards, but still know little about central auditory processing. We have therefore used Gekko gecko for further analysis of the lizard central auditory system.Geckos are auditory specialists that use vocalizations for intraspecific communication (Marcellini, 1977;Tang et al., 2001). Their ears differ from those of other reptiles such as archosaurs and turtles. Lepidosaur ears are highly directional, with middle ears connected through the mouth cavity (Christensen-Dalsgaard and Manley, 2008). This patent connection enhances the directionality of the ear by allowing sound access to both sides of NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript each tympanic membrane. The acoustically coupled ear creates directional responses from the tympanum Manley, 2005, 2008). Additionally, lizards have independently evolved micromechanical hair cell tuning, permitting emergence of sensitive high-frequency hearing in a specialized region of the papilla (Manley, 2002). Thus lizard auditory systems might reveal specializations for hearing high frequencies and sound localization.We have begun our analysis of liza...

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Laminar and Neurochemical Organization of the Dorsal Cochlear Nucleus of the Human, Monkey, Cat, and Rodents

Baizer

Wong

Paolone

et al. 2014

The Anatomical Record

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The dorsal cochlear nucleus (DCN) is a brainstem structure that receives input from the auditory nerve. Many studies in a diversity of species have shown that the DCN has a laminar organization and identifiable neuron types with predictable synaptic relations to each other. In contrast, studies on the human DCN have found a less distinct laminar organization and fewer cell types, although there has been disagreement among studies in how to characterize laminar organization and which of the cell types identified in other animals are also present in humans. We have reexamined DCN organization in the human using immunohistochemistry to analyze the expression of several proteins that have been useful in delineating the neurochemical organization of other brainstem structures in humans: nonphosphorylated neurofilament protein (NPNFP), nitric oxide synthase (nNOS), and three calcium-binding proteins. The results for humans suggest a laminar organization with only two layers, and the presence of large projection neurons that are enriched in NPNFP. We did not observe evidence in humans of the inhibitory interneurons that have been described in the cat and rodent DCN. To compare humans and other animals directly we used immunohistochemistry to examine the DCN in the macaque monkey, the cat, and three rodents. We found similarities between macaque monkey and human in the expression of NPNFP and nNOS, and unexpected differences among species in the patterns of expression of the calcium-binding proteins.

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Characterization of cochlear nucleus principal cells of Meriones unguiculatus and Monodelphis domestica by use of calcium-binding protein immunolabeling

Cited by 38 publications

References 98 publications

Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

Calcium‐binding protein immunoreactivity characterizes the auditory system of Gekko gecko

Laminar and Neurochemical Organization of the Dorsal Cochlear Nucleus of the Human, Monkey, Cat, and Rodents

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