Characterization of codfish gelatin: A comparative study of fresh and salted skins and different extraction methods

Alves, Ana L.; Fraguas, Francisco Javier; Carvalho, Ana C.; Valcárcel, Jesús; Pérez‐Martín, Ricardo I.; Reis, Rui L.; Vázquez, José Antonio; Silva, Tiago H.

doi:10.1016/j.foodhyd.2021.107238

Cited by 19 publications

(11 citation statements)

References 59 publications

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“…The gelatin chain structure obtained by the extraction method from tuna collagen was characterized by GPC and TGA. The GPC eluogram of tuna gelatin (Dry GE) exhibits several overlapping polymeric peaks, starting at 38 min with the elution of high Mw species, up to around 60 min when light scattering signals return to baseline (Figure 2) which is in agreement with skin gelatin from other fish species obtained using an identical extraction process [23][24][25][26]. DOX is a multiring planar molecule, which can provide appropriate binding sites through π-π and hydrogen bonding interactions on the transport of other guest molecules.…”

Section: Resultssupporting

confidence: 78%

Characterization of Tuna Gelatin-Based Hydrogels as a Matrix for Drug Delivery

Hermida-Merino

Cabaleiro

Lugo

et al. 2022

Gels

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The skin of yellowfin tuna is one of the fishery industry solid residues with the greatest potential to add extra value to its circular economy that remains yet unexploited. Particularly, the high collagen content of fish skin allows generating gelatin by hydrolysis, which is ideal for forming hydrogels due to its biocompatibility and gelling capability. Hydrogels have been used as drug carriers for local administration due to their mechanical properties and drug loading capacity. Herein, novel tuna gelatin hydrogels were designed as drug vehicles with two structurally different antitumoral model compounds such as Doxorubicin and Crocin to be administrated locally in tissues with complex human anatomies after surgical resection. The characterization by gel permeation chromatography (GPC) of purified gelatin confirmed their heterogeneity composition, exhibiting three major bands that correspond to the β and α chains along with high molecular weight species. In addition, the Fourier Transform Infrared (FT-IR) spectra of gelatin probed the secondary structure of the gelatin showing the simultaneous existence of α helix, β sheet, and random coil structures. Morphological studies at different length scales were performed by a multi-technique approach using SAXS/WAXS, AFM and cryo-SEM that revealed the porous network formed by the interaction of gelatin planar aggregates. In addition, the sol-gel transition, as well as the gelation point and the hydrogel strength, were studied using dynamic rheology and differential scanning calorimetry. Likewise, the loading and release profiles followed by UV-visible spectroscopy indicated that the novel gelatin hydrogels improve the drug release of Doxorubicin and Crocin in a sustained fashion, indicating the structure-function importance in the material composition.

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Section: Resultssupporting

confidence: 78%

Characterization of Tuna Gelatin-Based Hydrogels as a Matrix for Drug Delivery

Hermida-Merino

Cabaleiro

Lugo

et al. 2022

Gels

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“…Table 4 shows the yields obtained from the different tench residues. The chemical composition of fish gelatin prepared from the skin had a yield that reached 11.82%, which was lower than the results obtained by other researchers [ 12 ], who reported an 18.52% yield from skin waste in the best extractive conditions. The 40% decrease in yield may be due to a less careful separation of the skin from the muscle compared to the abovementioned studies and a worse thermal treatment, which, as the study itself explains through response surface models, is one of the factors that most alters the extraction process.…”

Section: Resultscontrasting

confidence: 74%

“…Gelatin extraction from the skin was completed with some modifications [ 12 ]. The clean skins were soaked in NaOH (0.2%) (4:1 w / v ) for thirty minutes, with mild stirring, at 22 °C, followed by washing in distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…Gelatin is a colloidal protein with unique characteristics. Much of its value lies in its inherent ability to form thermo-reversible gels when combined with water, a property that offers it a functionality that makes it a very versatile polymer for multiple industries, such as pharmaceuticals, cosmetics, and foods [ 12 ]. Gelatin properties depend on several factors, such as animal species, age, source tissues, and extraction conditions [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Gelatin is divided into two types: type A (obtained under acidic conditions) and type B (obtained under alkaline conditions). A higher yield is obtained when the extraction process is carried out with acid solutions, since the acid converts the triple helix of collagen into single chains, while the alkaline solutions transform it into double chains [ 12 ]. In addition, the method used to remove moisture is fundamental to obtaining gels with good mechanical properties, which is also key in industrial scale-up due to the high economic costs it generates [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Optimization of the Extraction of Chitosan and Fish Gelatin from Fishery Waste and Their Antimicrobial Potential as Active Biopolymers

Rocha-Pimienta

Navajas-Preciado

Barraso-Gil

et al. 2023

Gels

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Fishery residues are abundant raw materials that also provide numerous metabolites with high added value. Their classic valorization includes energy recovery, composting, animal feed, and direct deposits in landfills or oceans along with the environmental impacts that this entails. However, through extraction processes, they can be transformed into new compounds with high added value, offering a more sustainable solution. The aim of this study was to optimize the extraction process of chitosan and fish gelatin from fishery waste and their revalorization as active biopolymers. We successfully optimized the chitosan extraction process, achieving a yield of 20.45% and a deacetylation degree of 69.25%. For the fish gelatin extraction process, yields of 11.82% for the skin and 2.31% for the bone residues were achieved. In addition, it was demonstrated that simple purification steps using activated carbon improve the gelatin’s quality significantly. Finally, biopolymers based on fish gelatin and chitosan showed excellent bactericidal capabilities against Escherichia coli and Listeria innocua. For this reason, these active biopolymers can stop or decrease bacterial growth in their potential food packaging applications. In view of the low technological transfer and the lack of information about the revalorization of fishery waste, this work offers extraction conditions with good yields that can be easily implemented in the existing industrial fabric, reducing costs and supporting the economic development of the fish processing sector and the creation of value from its waste.

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