2021
DOI: 10.3389/fimmu.2021.641664
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Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach

Abstract: Coelomocytes is the generic name for a collection of cellular morphotypes, present in many coelomate animals, and highly variable among echinoderm classes. The roles attributed to the major types of these free circulating cells present in the coelomic fluid of echinoderms include immune response, phagocytic digestion and clotting. Our main aim in this study was to characterize coelomocytes found in the coelomic fluid of Marthasterias glacialis (class Asteroidea) by using a combination of flow cytometry (FC), i… Show more

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Cited by 15 publications
(17 citation statements)
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“…The P3 cells differ from P1 cells in terms of DRAQ5 signal intensity and BF size features and differ from P2 cells mostly in BF size features. The two P1 morphotypes previously described by fluorescence microscopy (Andrade et al 2021) were also discriminated by the IA algorithm. Here, the P1S morphotype presents a smaller nucleus-to-cytoplasm ratio and a heavily granulated cytoplasm, surrounded by an uninterrupted bright red membrane.…”
Section: Characterization Of Coelomocytes Populations and Of Their Mo...mentioning
confidence: 99%
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“…The P3 cells differ from P1 cells in terms of DRAQ5 signal intensity and BF size features and differ from P2 cells mostly in BF size features. The two P1 morphotypes previously described by fluorescence microscopy (Andrade et al 2021) were also discriminated by the IA algorithm. Here, the P1S morphotype presents a smaller nucleus-to-cytoplasm ratio and a heavily granulated cytoplasm, surrounded by an uninterrupted bright red membrane.…”
Section: Characterization Of Coelomocytes Populations and Of Their Mo...mentioning
confidence: 99%
“…These populations are present in similar proportions in all experimental groups, both in the control condition and in the nerve regenerating samples (at all time-points). When analyzed previously (Andrade et al 2021), two morphotypes of the haploid and low mitotic active P1 cell population were detected. One with a less heterogeneous nucleus and a higher nucleus-to-cytoplasm ratio, was hypothesized to be a non-differentiated form of the more mature morphotype.…”
Section: A New Coelomocyte Population Was Detected Trough Fc During R...mentioning
confidence: 99%
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“…Echinoderm immune cells are the so-called coelomocytes, a heterogeneous population of cells freely circulating in all coelomic spaces, including the perivisceral coelomic cavities, the open water-vascular system, the perihemal system, but also in the main tissues and organs (95)(96)(97). A general consensus on echinoderm coelomocyte types is lacking, possibly due to the actual diversity of morphotypes among the different classes ( 93) but mostly to the diversity of techniques and protocols used to analyze them (98). For this reason, from two to up to twelve cytotypes are reported in the literature (97,(99)(100)(101)(102)(103)(104)(105).…”
Section: Echinodermsmentioning
confidence: 99%